Short communication: The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis

Catia Pereira, Itziar A. Montalbán, Tomás Goicoa, M. Dolores Ugarte, Sandra Correia, Jorge M. Canhoto, Paloma Moncaleán


Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis.

Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain).

Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage.

Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages.

Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. 


embryogenic cell lines; Pinus halepensis; somatic embryos; water availability

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DOI: 10.5424/fs/2017263-11436