Seed germination of Pinus koraiensis in response to light regimes caused by shading and seed positions

M. Zhang, J. Zhu, Q. Yan

Abstract


Pinus koraiensis Siebold & Zucc. (Korean pine), the dominant tree species in the mixed broadleaved Korean pine forests (regional climax), is severely restricted by its regeneration failure. To determine the effects of light regimes on P. koraiensis regeneration, the seed germination process was examined in shade houses and forest stands (before and after leaf expansion) with various light levels created by shading and seed positions. Despite the large size of P. koraiensis seeds (500-600 mg), both light intensity and quality significantly affected the germination percentage in both shade houses and forests. Substantial changes in light intensity and quality led the majority of seeds (80%) to germinate in leafless forests and shade houses, while only a minority (≤ 20%) germinated after leaf expansion in the forests. Moreover, seed germination in shade houses and leafless forests exhibited similar patterns; they consistently reached a 70% shading degree, which was optimal for the seed germination of P. koraiensis on topsoil. Seed positioning significantly affected germination for each shading degree, especially when litter and soil coverings drastically inhibited germination. In conclusion, (1) when seeds were not stressed by temperature and moisture, light irradiance played a critical role in the seed germination of P. koraiensis; (2) seed positioning, in relation to alterations in light intensity and quality, affected the germination of P. koraiensis; (3) a shade house experiment using neutral cloth provided an applicable and controllable way to monitor the P. koraiensis seed germination in early spring before leaf expansion. The light requirement for the germination of P. koraiensis played a key role in the regeneration of P. koraiensis throughout the temperate secondary forests.


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References


Argyris J, Dahal P, Hayashi E, Still DW, Bradford KJ. 2008. Genetic variation for lettuce seed thermoinhibition is associated with temperature-sensitive expression of abscisic acid, gibberellin and ethylene biosynthesis, metabolism and response genes. Plant Physio 148, 926-947. http://dx.doi.org/10.1104/pp.108.125807 PMid:18753282 PMCid:2556833

Burgin MJ, Casal JJ, Whitelam GC, Sanchez RA. 1999. A light-regulated pool of phytochrome and rudimentary high-irradiance responses under far-red light in Pinus elliottii and Pseudotsuga menziessii. J Exp Bot 50, 831-836.

Castro J, Zamora R, Hódar JA. 2002. Mechanisms Blocking Pinus Sylvestris Colonization of Mediterranean Mountain Meadows. J Veg Sci 13, 725-731.

Du XJ, Guo QF, Gao XM, Ma KP. 2007. Seed rain, soil loss and regeneration of Castanopsis fargesii (Fagaceae) in a subtropical evergreen broad-leaved forest. For Ecol Manag 238, 212-219.

Facelli JM, Pickett STA. 1991. Plant litter: light interception and effects on an old-field plant community. Ecology 72, 1024-1031.

http://dx.doi.org/10.2307/1940602

Farrell C, Szota C, Hobbs RJ, Colmer TD. 2011. Microsite and litter cover effects on soil conditions and seedling recruitment in a saline agricultural system. Plant Soil 348, 397-409. http://dx.doi.org/10.1007/s11104-011-0850-7

Griffith TM, Sultan SE. 2005. Shade tolerance plasticity in response to neutral vs green shade cues in Polygonum species of contrasting ecological breadth. New Phytol 166, 141-148. http://dx.doi.org/10.1111/j.1469-8137.2004.01277.x PMid:15760358

Grill R, Spruit CJP. 1972. Properties of phytochrome in gymnosperms. Planta 108, 203-213. http://dx.doi.org/10.1007/BF00384109

Hao ZQ, Zhang J, Song B, Ye J, Li BH. 2007. Vertical structure and spatial associations of dominant tree species in an old-growth temperate forest. For Ecol Manag 252, 1-11.

Holmes MG, Smith H. 1977. The function of phytochrome in the natural environment–II. The influence of vegetation canopies on the spectral energy distribution of natural daylight. Photochem Photobio 25, 539-545.

http://dx.doi.org/10.1111/j.1751-1097.1977.tb09125.x

Hutchins HE, Hutchins SA, Liu BW. 1996. The role of birds and mammals in Korean pine (Pinus koraiensis) regeneration. Oecologia 107, 120-130. http://dx.doi.org/10.1007/BF00582242

Kyereh B, Swaine MD, Thompson J. 1999. Effect of light on the germination of forest trees in Ghana. J Ecol 87, 772-783. http://dx.doi.org/10.1046/j.1365-2745.1999.00386.x

Li X, Xu ZB, Tao DL. 1989. Natural Regeneration of Korean Pine in Broadleaved-Korean Pine Stands on Fenglin Natural Reserve of Xiaoxing' Anling. Journal of Northeast Forestry University 17(6), 1-7.

Ma JL, Zhuang LW. 1992. The geographic distribution of Pinus koraiensis. Journal of Northeast Forestry University 20, 40-48.

Makana JR, Thomas SC. 2005. Effects of light gaps and litter removal on the seedling performance of six African timber species. Biotropica 37, 227-237. http://dx.doi.org/10.1111/j.1744-7429.2005.00030.x

Mamo N, Mihretu M, Fekadu M, Tigabu M, Teketay D. 2006. Variation in seed and germination characteristics among Juniperus procera populations in Ethiopia. For Ecol Manag 225: 320-327.

Milberg P, Andersson L, Thompson K. 2000. Large-seeded species are less dependent on light for germination than small-seeded ones. Seed Sci Res 10, 99-104.

http://dx.doi.org/10.1017/S0960258500000118

Molofsky J, Augspurger CK. 1992. The effect of leaf litter on early seedling establishment in a tropical forest. Ecology 73, 68-77. http://dx.doi.org/10.2307/1938721

Muscolo A, Sidari M. 2006. Seasonal fluctuations in soil phenolics of a coniferous forest: effects on seed germination of different coniferous species. Plant Soil 284, 305- 318. http://dx.doi.org/10.1007/s11104-006-0040-1

Parker WC, Noland TL, Momeault AE. 2006. The effects of seed mass on germination, seedling emergence, and early seedling growth of eastern white pine (Pinus strobus L.). New Forest 32, 33-49. http://dx.doi.org/10.1007/s11056-005-3391-1

Pearson TRH, Burslem DFRP, Mullins CE, Dalling JW. 2002. Germination ecology of neotropical pioneers: interacting effects of environmental conditions and seed size. Ecology 83, 2798-2807. http://dx.doi.org/10.1890/0012-9658(2002)083[2798:GEONPI]2.0.CO;2

Pons TL. 1983. Significance of inhibition of seed germination under the leaf canopy in ash coppice. Plant Cell Environ 6, 385-392. http://dx.doi.org/10.1111/j.1365-3040.1983.tb01271.x

Pons TL. 1992. Seed responses to light. In: Seeds: the ecology of regeneration in plant communities (Fenner M, ed). CAB international, Wallingford, UK. pp. 259-284.

Rincón E, Huante P. 1993. Growth responses of tropical deciduous tree seedlings to contrasting light conditions. Tree 7, 202-207.

Ruano I, Pando V, Bravo F. 2009. How do light and water influence Pinus pinaster Ait. germination and early seedling development? For Ecol Manag 258, 2647-2653.

Seiwa K, Ando M, Imaji A, Tomita M, Kanou K. 2009. Spatio-temporal variation of environmental signals inducing seed germination in temperate conifer plantations and natural hardwood forests in Northern Japan. For Ecol Manag 257, 361-369.

Smith H. 1982. Light quality, photoperception, and plant strategy. Annu Rev Plant Physiol 33, 481-518. http://dx.doi.org/10.1146/annurev.pp.33.060182.002405

Smith M, Cappelle J. 1992. Effects of soil surface microtopography and litter cover on germination, growth and biomass production of chicory (Cichorium intybus L.). Am Midl Nat 128, 246-253. http://dx.doi.org/10.2307/2426458

Tanaka-Oda A, Kenzo T, Fukuda K. 2009. Optimal germination condition by sulfuric acid pretreatment to improve seed germination of Sabina vulgaris Ant. J For Res 14, 251-256. http://dx.doi.org/10.1007/s10310-009-0129-5

Thanos CA, Skordilis A. 1987. The effects of light, temperature and osmotic stress on the germination of Pinus halepensis and P. brutia seeds. Seed Science and Technology- Netherlands 15, 163-174.

Tillman-Sutela E, Kauppi A, Karppinen K, Tomback DF. 2008. Variant maturity in seed structures of Pinus albicaulis (Engelm.) and Pinus sibirica (Du Tour): key to a soil seed bank, unusual among conifers? Tree 22, 225-236.

Toole VK, Toole EH, Borthwick HA, Snow AG. 1962. Responses of seeds of Pinus taeda & P. strobus to light. Plant Physiol 37, 228-233. http://dx.doi.org/10.1104/pp.37.2.228 PMid:16655635 PMCid:549766

Vázquez-Yanes C, Orozco-Segovia A. 1992. Effects of litter from a tropical rainforest on tree seed germination and establishment under controlled conditions. Tree Physiol 11, 391-400. Williams CE, Lipscomb MV, Johnson WC, Nilsen ET. 1990. Influence of leaf litter and soil moisture regime on early establishment of Pinus pungens. Am Midl Nat 124, 142-152.

Wu XP, Zhu B, Zhao SQ, Pu SL, Fang JY. 2004. Comparison of community structure and species diversity of mixed forests of deciduous broad-leaved tree and Korean pine in northeast China. Biodiversity Science 12, 174-181. [In Chinese with English abstract].

Xiong S, Nilsson C. 1999. The effect of plant litter on vegetation: a meta-analysis. J Ecol 87, 984-994. http://dx.doi.org/10.1046/j.1365-2745.1999.00414.x

Yirdaw E, Leinonen K. 2002. Seed germination responses of four afromontane tree species to red/far-red ratio and temperature. For Ecol Manag 168, 53-61.

Yu LL, Zhu JJ, Kong XW, Hu WL, Tan XR. 2006. The effects of anthropogenic disturbance (thinning) on plant Ecologica Sinica 26, 3757-3564.

Zhu JJ, Kang HZ, Tan H, Xu ML. 2006. Effects of drought stresses induced by polyethylene glycol on germination of Pinus sylvestris var. mongolica seeds from natural and plantation forests on sandy land. J For Res 11, 319- 328. http://dx.doi.org/10.1007/s10310-006-0214-y

Zhu JJ, Liu ZG, Wang HX, Yan QL, Fang HY, Hu LL, et al. 2008. Effects of site preparation on emergence and early establishment of Larix olgensis in montane regions of northeastern China. New forest 36, 247-260.

Zhu JJ, Matsuzaki T, Lee FQ, Gonda Y. 2003. Effects of gap size created by thinning on seedling emergency, survival and establishment in a coastal pine forest. For Ecol Manag 182, 339-354.




DOI: 10.5424/fs/2012213-02721

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