Effect of seed mass and number of cotyledons on seed germination after heat treatment in Pinus sylvestris L. var. iberica Svob.

  • Elena Castoldi Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid.
  • Jose A. Molina Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid.

Abstract

Aim of study: We investigated the combined effect of seed mass and number of cotyledons on the seed germination of Pinus sylvestris var. iberica (Iberian Scots pine) in simulated fire conditions.

Material and Methods: We used 3,600 fresh seeds extracted from 158 cones obtained from 10 pine trees located at the Biological Station of the Complutense University (Guadarrama mountains, Madrid region). All the seeds were individually weighed and assigned to one of the two following seed mass classes: class I (1.6-12.5 mg) and class II (12.6-145.0 mg). Germination capacity (GC) and mean germination time (MGT) were studied in combined experiments of four different temperatures (100°, 125°, 150° and 175°C) and two exposure times (1 and 5 minutes) together with a control (no treatment). Four replicates of 50 seeds each were used for each treatment and hydrated daily for 14 days to germinate under constant illumination. The number of cotyledons was counted in seedlings.

Main results: Germination was depressed at above 125°C for 5 min. GC and MGT were negatively related, and were influenced by temperature and exposure time. Seed mass was found to have a significant effect on GC at some moderate heat treatment but not on MGT. The number of cotyledons was positively correlated to seed mass but there was not found correlated with germination after seed heat treatments.

Research highlights: In the case of the Iberian Scots pine, higher seed mass mitigate the negative effects of temperature on seed germination after moderate heat treatment simulating fire.

Keywords: Fire disturbance; germination; Mediterranean mountains; Pinus sylvestris L. var. Iberica; temperature.

 

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Author Biographies

Elena Castoldi, Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid.
PhD student
Jose A. Molina, Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid.
Professor

References

Amaral Franco J, 1986. Pinus L. In: Flora Iberica (Castroviejo S et al., eds). CSIC, Madrid, Spain. Vol.1: 168-174.

Bewley JD, Black M, 1994. Seeds: Physiology of Development and Germination. Plenum press, New York, USA. http://dx.doi.org/10.1007/978-1-4899-1002-8

Bladé C, Vallejo VR, 2008. Seed mass effects on performance of Pinus halepensis Mill. seedlings sown after fire. For Ecol Manage 255: 2362-2371.

Buchholz JT, 1946. Volumetric studies of seeds, endosperms and embryos in Pinus ponderosa during embryonic differentiation. Bot Gaz 108: 232-244. http://dx.doi.org/10.1086/335409

Cañellas I, Martinez-Garcia F, Montero G, 2000. Silviculture and dynamics of Pinus sylvestris L. stands in Spain. Invest Agr Sist Recur For 1: 1-21.

Castoldi E, Molina JA, 2012. Vegetation composition of the UCM Biological Field Station "Finca de Ontalba". Obs Medioamb 15: 115-123.

Castoldi E., Quintana J.R., García M.R. and Molina J.A, 2013. Early post-fire plant succession in slash-pile prescribed burns of a sub-Mediterranean managed forest. Plant Ecol Evol 146: 272-278. http://dx.doi.org/10.5091/plecevo.2013.848

Castro J, 1999. Seed mass versus seedling performance in Scots pine: a maternally dependent trait. New Phytol 144: 153-161. http://dx.doi.org/10.1046/j.1469-8137.1999.00495.x

Come D, 1970. Les obstacles à la germination. Masson et Cie, Paris, France.

Debain S, Curt T, Lepart J, 2003. Seed mass, seed dispersal capacity and seedling performance en a Pinus sylvestris population. Ecoscience 10: 168-175.

Elias Castillo F, Ruiz Beltrán L, 1977. Agroclimatología de Espa-a. INIA. Cuaderno nº 7, Ministerio de Agricultura, Spain.

Escudero A, Barrero S, Pita JM, 1997. Effects of high temperatures and ash on seed germination of two Iberian pines (Pinus nigra ssp salzmannii, P. sylvestris var iberica). Ann For Sci 54: 553-562. http://dx.doi.org/10.1051/forest:19970605

Guerra A, Guitián F, Paneque G, García A, Sánchez JA, Monturiol F, Mudarra JL, 1966. Mapa de suelos de España, escala 1: 1.000.000. CISC, Madrid, Spain.

Habrouk A, Retana J, Espelta JM, 1999. Role of heat tolerance and cone protection of seeds in the response of three pine species to wildfires. Plant Ecol 145: 91-99. http://dx.doi.org/10.1023/A:1009851614885

Hille M, den Ouden J, 2004. Improved recruitment and early growth of Scots pine (Pinus sylvestris L.) seedlings after fire and soil scarification. Eur J For Res 123: 213-218. http://dx.doi.org/10.1007/s10342-004-0036-4

Isik K, 1985. Altitudinal variation in Pinus brutia Ten.: Seed and seedling characteristics. Silvae Genet 35: 58-67.

Martínez J, Vega-García C, Chuvieco E, 2009. Human-caused wildfire risk rating for prevention planning en Spain. J Environ Manage 90: 1241-1252. http://dx.doi.org/10.1016/j.jenvman.2008.07.005

Mikola J, 1985. Relationship between height growth differences of Scots pine full-sib families and variation in seed size, annual growth rhythm, and some foliage characteristics. (Tiegerstedt PMA, Puttonen P, Koski V, eds). Helsinki University Press, Helsinki, Finland, pp: 233-243.

Mirov NT, 1967. The Genus Pinus. Ronald Press, New York, USA. 602 pp.

Montero G, Rojo A, Álvarez MF, Del Río M, 2001a. Aspectos selvícolas y económicos de los pinares de Pinus sylvestris L. en el Sistema Central. Estudios Agrosociales y Pesqueros 193: 27-56.

Montero G, Cañellas I, Ortega C, Del río M, 2001b. Results from a thinning experiment in a Scots pine (Pinus sylvestris L.) natural regeneration stand in the Sistema Ibérico Mountain Range (Spain). For Ecol Manage 145: 151-161.

Nygren M, 1987. Germination characteristics of autumn collected Pinus sylvestris seeds. Acta forestalia fennica 201, Helsinki, Finland.

Núñez MR, Calvo L, 2000. Effect of high temperatures on seed germination of Pinus sylvestris and Pinus halepensis. For Ecol Manage 13: 183-190.

Palahí M, Miina J, Tomé M, Montero G, 2002. Stand-level yield model for Scots pine (Pinus sylvestris L.) in north-east Spain. Invest Agr Sist Recur For 11: 409-424.

Parker WC, Noland TL, Morneault AE, 2006. The effects of seed mass on germination, seedling emergence, and early seedling growth of eastern white pine (Pinus strobus L.) New Forest 32, 33-49. http://dx.doi.org/10.1007/s11056-005-3391-1

Peinado M, Rivas-Martínez S, 1987. La vegetación de España. Serv. Publ. Univ. Alcalá, Madrid, Spain. 544 pp.

Reich PB, Oleksyn J, Tjoelker MG, 1994. Seed mass effects on germination and growth of diverse European Scots pine populations. Can J For Res 24: 306-320. http://dx.doi.org/10.1139/x94-044

Reyes O, Casal M, 1995. Germination behaviour of 3 species of the genus Pinus in relation to high temperatures suffered during forest fires. Ann For Sci 52: 385-392. http://dx.doi.org/10.1051/forest:19950408

Roach DA, Wulff RD, 1987. Maternal effects in plants. Ann Rev Ecol Systematics 18: 209-235. http://dx.doi.org/10.1146/annurev.es.18.110187.001233

Seifert K, Morgan-Jones G, Gams W, Kendrick B, 2011. The Genera of Hyphomycetes. CBS Biodiversity Series 9. CBS-KNAW Fungal Biodiversity Centre, Utrecht, Holand.

Simak M, Gustafsson, Å, 1954. Seed properties in mother trees and grafts of Scots pine. Statens Skogsfor 44: 1-80.

Squillace A.E. 1964. Geographic variation in Slash pine (Pinus elliottii Engelm.). PhD Dissertation. University of Florida, USA.

Tapias R, Climent J, Pardos JA, Gil L, 2004. Life histories of Mediterranean pines. Plant Ecol 171: 53-68. http://dx.doi.org/10.1023/B:VEGE.0000029383.72609.f0

Tíscas OP, Lucas BM, 2010. Seed mass variation, germination time and seedling performance in a population of Pinus nigra subsp. salzamannii. For Syst 3: 344-353.

Wennström U, Bergsten U, Nilsson JE, 2002. Effects of seed weight and seed type on early seedling growth of Pinus sylvestris under harsh and optimal conditions. Scand J For Res 17: 118-130. http://dx.doi.org/10.1080/028275802753626764

Yeatman CW, 1966. Germinant size of jack pine in relation to seed size and geographic origin. Joint proceeding of the second genetics workshop of the Society of American Foresters and the seventh lake states forest tree improvement conference; Res. Pap. NC-6. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station. 28-36 pp.

Zaborovskii EP, 1996. Analysis of cones and seeds of a single Scots pine tree. Lesn. Zh. (Archangel, USSR) 9: 162-163 [In Russian].

Published
2014-12-01
How to Cite
Castoldi, E., & Molina, J. A. (2014). Effect of seed mass and number of cotyledons on seed germination after heat treatment in Pinus sylvestris L. var. iberica Svob. Forest Systems, 23(3), 483-489. https://doi.org/10.5424/fs/2014233-05480
Section
Research Articles