Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

María F. Pomponio, Cintia Acuña, Vivien Petreath, Diego L. Lauenstein, Susana M. Poltri, Susana Torales

Abstract


Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus.

Area of the study: samples were obtained from natural populations of Argentina.

Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids.

Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci.

Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.

Keywords: genetic variation; functional markers; microsatellites; prosopis.

Abbreviations: PIC: Polymorphic Information Content; PCR: Polymerase Chain Reaction; SSR: Simple Sequence Repeat.


Full Text:

PDF HTML XML

References


References

Alves FM, Zucci MI, Azevedo-Tozzi AM, Sartori ALB, SOUZA AP, 2014. Characterization of microsatellite markers developed from Prosopis rubriflora and Prosopis ruscifolia (Leguminosae-Mimosoideae), legume species that are used as models for genetic diversity studies in Chaquenian areas under anthropization in South America. BMC Research Notes 7: 375. http://dx.doi.org/10.1186/1756-0500-7-375

Bessega CF, Pometti CL, Miller JT, Watts R, Saidman BO, Vilardi JC, 2013. New Microsatellite loci for Prosopis alba and P. chilensis (Fabaceae). Applications in Plant Sciences 5: 1200324. http://dx.doi.org/10.3732/apps.1200324

Burkart A, 1976. A monograph of the genus Prosopis (Leguminosae subfam. Mimosoideae). J Arnold Arbor. 57: 219-249.

Cabrera AL, 1976. Regiones Fitogeográficas Argentinas. In Enciclopedia Argentina de Agricultura y Jardinería. Ed. W. F. Kugler. Editorial ACME, Buenos Aires. 85 pp.

Mottura M, Finkeldey R, Verga A, Gailing O, 2005. Development and characterization of microsatellite markers for Prosopis chilensis and Prosopis flexuosa and cross-species amplification. Mol Ecol Notes 5: 487–489. http://dx.doi.org/10.1111/j.1471-8286.2005.00965.x

Park SDE, 2001. Trypanotolerance in West African Cattle and the Population Genetic Effects of Selection. Ph.D. thesis. University of Dublin, Ireland. Exel Microsatellite Toolkit.

Pasiecznik N M, Felker P, Harris P, Harsh LN, Cruz G, Tewari JC, Cadoret K, Maldonado L, 2001. The Prosopis juliflora–Prosopis pallida complex: a monograph. HDRA, Coventry, UK. 172 pp.

Peakall R, Smouse PE, 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28: 2537-2539. http://dx.doi.org/10.1093/bioinformatics/bts460

Rousset F, 2008. Genepop'007: a complete reimplementation of the Genepop software for Windows and Linux. Mol. Ecol. Resources 8: 103-106. http://dx.doi.org/10.1111/j.1471-8286.2007.01931.x

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol 28: 2731-2739. http://dx.doi.org/10.1093/molbev/msr121

Torales S, Rivarola ML, Pomponio MF, González S, Acuña CV, Fernández P, Lauenstein D L, Verga A, Hopp EH, Paniego NB, Marcucci Poltri SN, 2013. De novo assembly and characterization of leaf transcriptome for the development of functional molecular markers of the extremophile multipurpose tree species Prosopis alba. BMC Genomics 14: 705. http://dx.doi.org/10.1186/1471-2164-14-705

Varshney RK, Gramer A, Sorrells ME, 2005. Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23: 48-55. http://dx.doi.org/10.1016/j.tibtech.2004.11.005




DOI: 10.5424/fs/2015242-07188

Webpage: www.inia.es/Forestsystems