Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

María F. Pomponio, Cintia Acuña, Vivien Petreath, Diego L. Lauenstein, Susana M. Poltri, Susana Torales


Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus.

Area of the study: samples were obtained from natural populations of Argentina.

Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids.

Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci.

Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.

Keywords: genetic variation; functional markers; microsatellites; prosopis.

Abbreviations: PIC: Polymorphic Information Content; PCR: Polymerase Chain Reaction; SSR: Simple Sequence Repeat.

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DOI: 10.5424/fs/2015242-07188