The Canadian Ophiostoma genome project

  • L. Bernier Université Laval
  • C. Breuil University of British Columbia
  • W. E. Hintz University of Victoria
  • P. A. Horgen University of Toronto
  • V. Jacobi Université Laval
  • J. Dufour Université Laval
  • M. Aoun Université Laval
  • G. Bouvet Université Laval
  • S. H. Kim University of British Columbia
  • S. Diguistini University of British Columbia
  • P. Tanguay University of British Columbia
  • J. Eades University of Victoria
  • S. Burgess University of Victoria
  • P. de la Bastide University of Victoria
  • M. Pinchback University of Victoria
  • Y. Tadesse University of Toronto
DOI: https://doi.org/10.5424/818
Keywords: Ophiostoma, Dutch elm disease, sapstain, functional genomics, expressed sequenced tag, genetic mapping, bioinformatics

Abstract

The Canadian Ophiostoma Genome Project, which was initiated in 2001, is a collaborative effort between research teams in four different universities. Its general objective is to conduct a large-scale identification and analysis of genes controlling important aspects of the life cycle of Ophiostomatoid fungi. To this end, several expressed sequence tag (EST) libraries were obtained for the Dutch elm disease pathogen Ophiostoma novo-ulmi and the sapstainer O. piceae, following partial, single-pass automated sequencing of complementary DNA clones. The largest EST library, prepared from yeast like cells of O. novo-ulmi grown at 24 °C, contains over 3,400 readable sequences and serves as a general reference library for Ophiostomatoid fungi. Smaller, specific EST libraries were constructed from mycelia of O. novo-ulmi grown at suboptimal temperatures, from perithecia formed in laboratory crosses, as well as from O. piceae grown on different carbon sources. Ongoing bioinformatic searches in public databases have so far identified over 750 Ophiostoma unique ESTs which show significant homology with other fungal genes of known function, although a high proportion of Ophiostoma ESTs are either orphans (no match to any known gene) or show homology to genes of unknown function. In addition to EST analysis, differential expression of selected genes and structural genomics are also being studied.

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Published
2004-04-01
How to Cite
Bernier, L., Breuil, C., Hintz, W. E., Horgen, P. A., Jacobi, V., Dufour, J., Aoun, M., Bouvet, G., Kim, S. H., Diguistini, S., Tanguay, P., Eades, J., Burgess, S., de la Bastide, P., Pinchback, M., & Tadesse, Y. (2004). The Canadian Ophiostoma genome project. Forest Systems, 13(1), 105-117. https://doi.org/10.5424/818
Issue
Vol. 13 No. 1 (2004)
Section
Research Articles

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