Rooting of Mugo pine (Pinus mugo) cuttings as affected by IBA, NAA and planting substrate

Shahram Sedaghathoor, Saghi Kayghobadi, Yahya Tajvar


Aim of study: The effect of planting substrate and concentrations of indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) hormones was studied on the rooting of mugo pine cuttings.

Area of study: The research was carried out in Rasht city, Guilan province, Iran.

Material and Methods: Both hormones (IBA and NAA) were applied at four concentrations of 0, 1000, 2000 and 4000 mg/l. Planting substrates included sand, perlite, cocopeat, sand + perlite, and sand + cocopeat (1:1).

Main results: The highest rooting percentage (55%) was obtained under the trilateral treatment a2b4c1 (sand × 4000 mg/l NAA × 1000 mg/l IBA). Sand + cocopeat was found to be the best rooting substrate.

Research highlights: It is recommended to apply sand with 4000 mg/l and 1000mg/l concentration of experimental hormones (NAA and IBA, respectively).

Keywords: auxin; rooting; Pinus mugo; vegetative propagation.


Auxin, Rooting, Pinus mugo, Propagation

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Atkinson D, 1991. Plant root growth: an ecological perspective. Blackwell Scientific Publications, Oxford, UK. 478 pp.

Cole CJ, Dunn ED, 2002. Expanded polystyrene as substitute for perlite in rooting substrate. J Environ Hortic 20(1): 7–10.

Edward FG, Dennis GW, 1994. Pinus mugo (Mugo Pine). Southern Trees Fact sheet 467: 1-4.

Griffin JJ, Blazich FA, Ranney TG, 1999. Propagation of Magnolia virginiana 'Santa Rosa' by semi-hardwood cuttings. J Environ Hortic 17(1): 47–48.

Hammo YH, Abdul Kareem BZ, Salih MI, 2013. Effect of planting date and IBA concentration on rooting ability of stem cutting Of Fraser's Photinia (Photinia X Fraseri). IOSR J Agri Vet Sci 5(4): 51-54.

Hartmann HT, Kester DE, Davies FTJ, Geneve RL, 2011. Hartmann and Kester's Plant propagation, Principles and Practices, (eighth edition). Prentice Hall, Upper Saddle River, NJ, USA. 915 pp.

Haugh M, Moeller F, Roberts AN, 1974. Mugo pine cuttings rooted in June or January. Oregon Ornamental and Nursery Digest 18 (1): 4-6.

Hemati A, Khanjani Shiraz B, 2009. The study of the effects of soil and environment condition on Cupreessus macrocarpa rhizogenesis. Plant and Ecosys 5(20): 17-28.

Henrique A, Nogueira Campinhos E,Orika Ono E, Zambellode Pinho S, 2006. Effect of plant Growth Regulators in the Rooting of Pinus cutting. Braz Arch Biol Technol 49 (2): 189- 196.

Jorgensen H, 2010. NOBANIS - Invasive Alien species fact sheet - Pinus mugo. From: Online Database for the European Network on Invasive Alien Species. NOBANIS

MacDonald B, 2000. Practical woody plant propagation for nursery Growers. Timber Press, Portland, OR, USA. 660 pp.

Ofori DA, Newton AC, Leakey RRB, Grace J, 1996. Vegetative propagation of Milicia excelsa by leafy stem cuttings: Effects of auxin concentration, leaf area and rooting medium. For Ecol Manage 84(1-3): 39-48.

Reezi S, Naderi R, Khalighi A, Zamani Z, Etemad V, 2006. Asexual Propagation of Picea pungens 'Koster' through Cuttings, and grafting under Various Hormonal Treatments. J Iranian Nat Res 59 (3): 589 – 601.

Sadhu MK, 1998. Plant propagation. Wiley Eastern Limited, New Delhi, India. 287 pp.

Saffari M, Saffari VR, 2012. Effects of media and indole butyric acid (IBA) concentrations on hopbush (Dodoneae viscosa L.) cuttings in green house. Ann For Res 55(1): 61-68.

Štefančič M, Vodnik D, Štampar F, Osterc G, 2007. The effects of a fogging system on the physiological status and rooting capacity of leafy cuttings of woody species. Trees 21:491-496.

Wiesman Z, Lavee S, 1995. Enhancement of IBA stimulatory effect on rooting of olive cultivar stem cuttings. Sci Hortic 62 (3): 189-198.

Wiesman Z, Riov J, Epstein E, 1989. Paclobutrazol and urea-phosphate increase rooting and survival of peach Maravilhä softwood cuttings. Hort Sci 24(6): 908-909.

Wright RD, Rain WH, Virginia JRS, 1992. Propagation medium moisture level and rooting of woody stem cuttings. SNA Research Conference 37: 270- 273.

DOI: 10.5424/fs/2016252-09087