Effect of giberellic acid (GA4/7) and girdling on induction of reproductive structures in Pinus patula
Abstract
Aim of study: Seed production in forest tree species commonly takes a long time due to the length of the juvenile stage. Even though several treatments have been used to induce early flowering in conifer species, experience on their use in subtropical Pinus species is limited. This study aimed to evaluate the effect of GA4/7 dose (0, 1.27 and 2.54 mg cm-2) and application time (July-October), alone or in combination with partial stem girdling, on male and female strobili production in young Pinus patula Schiede ex Schltdl. et Cham. clones.
Area of study: Nine clones with different flowering background of high-elevation Pinus patula growing in a six-year old seed orchard established in Central México (Aquixtla, Puebla) at 2,800 m elevation.
Material and methods: Two independent flowering trials (FT1 and FT2) were carried out in the seed orchard during the 2009 and 2010 flowering cycles; similar factors were evaluated at both trials but time of application, clones tested, and experimental design used varied for each of them. Partial stem girdling was done at the base of the trunk and the GA4/7 solution was injected into the xylem above the point of girdling. The following spring, the percentage of trees with strobili and the number of strobili per tree were determined for both male and female structures.
Main results: Significant differences (p ≤ 0.05) among clones in flowering capacity were found at both trials. None of the treatments applied in FT1 resulted in an increase of strobili formation, most probably because they were applied too late in the growing season. In FT2, however, application of GA4/7 combined with partial stem girdling increased the percentage of trees with strobili and the number of strobili of both sexes, particularly when applied in early July. Partial stem girdling was more effective on promoting male strobili than female ones in gibberellin-treated grafts.
Research highlights: Timing of GA4/7 application and stem girdling was important, with a significant interaction with clones tested in FT2 on production of male strobili but not on female strobili. Application of 1.27 mg cm-2 GA4/7 and stem girdling in July promoted the highest percentage of trees with strobili, and increased 25-fold the number of female strobili and 5-fold the number of male strobili per tree as compared to the control treatment. Thus, operational use of this induction treatment would be valuable to increase and accelerate seed production in Pinus patula seed orchards in the region.
Keywords: early flowering; gibberellins; juvenile stage; seed orchard; seed production.
Abbreviations: GA: Gibberellic acid; GA4: Gibberellic acid 4; GA7: Gibberellic acid 7; GA4/7: Mix of gibberellic acids 4 and 7; FT1: Flowering trial carried out in the 2009 season; FT2: Flowering trial carried out in the 2010 season.Downloads
References
References
Almqvist C, 2003. Timing of GA4/7 application and the flowering of Pinus sylvestris grafts in the greenhouse. Tree Physiol 23: 413-418. http://dx.doi.org/10.1093/treephys/23.6.413
Chalupka W, 1980. Regulation of flowering in Scots pine (Pinus sylvestris L.) grafts by gibberellins. Silvae Genet 29: 118-121.
Cherry ML, Anekonda TS, Albrecht MJ, Howe GT, 2007. Flower stimulation in young miniaturized seed orchards of Douglas-fir (Pseudotsuga menziesii). Can J For Res 37: 1-10. http://dx.doi.org/10.1139/x06-199
Codesido V, Merlo E, 2007. Inducción floral mediante aplicación de GA4/7 y fertilización mineral en el huerto semillero de Pinus radiata D. Don de Sergude (Galicia). Inv Agraria: Sist Rec For 16: 262-266.
Daoust G, Plourde A, Beaulieu J, 1995. Influences of crown size and maturation on flower production and sex expression in Picea glauca treated with gibberellin A4/7. Tree Physiol 15: 471-475. http://dx.doi.org/10.1093/treephys/15.7-8.471
Dick JM, 1995. Flower induction in tropical and subtropical forest trees. Com For Rev 74(2): 115-120.
Dvorak WS, Hodge GR, Kietzka JE, Malan F, Osorio LF, Stanger TK, 2000. Pinus patula. In: Conservation and Testing of Tropical and Subtropical Forest Tree Species by the CAMCORE Cooperative, pp: 149-173. College of Natural Resources, NCSU. Raleigh, NC. USA.
Gómez-Cárdenas M, Vargas-Hernández JJ, Jasso-Mata J, Velázquez-Martínez A, Rodríguez-Franco C, 1998. Annual growth pattern of the terminal shoot in young trees of Pinus patula. Agrociencia 32: 357-364.
Greenwood MS, 1982. Rate, timing, and mode of gibberellin application for female strobilus production by grafted loblolly pine. Can J For Res 12: 998-1002. http://dx.doi.org/10.1139/x82-142
Harrison DL, Slee MU, 1991. Gibberellin A4/7 enhanced flowering in Pinus caribea var. hondurensis. Can J For Res 21: 788-793. http://dx.doi.org/10.1139/x91-111
Ho RH, Schnekenburger F, 1992. Gibberellin A4/7 promotes cone production on potted grafts of eastern white pine. Tree Physiol 11: 197-203. http://dx.doi.org/10.1093/treephys/11.2.197
Ho RH, Eng K, 1995. Promotion of cone production on field-grown eastern white pine grafts by gibberellin A4/7 application. For Ecol Manage75: 11-16.
Infante GS, Zárate LGP, 2000. Métodos Estadísticos; un Enfoque Interdisciplinario. Ed. Trillas, México. 643 pp.
Isaza N, 2008. Flower promotion in Pinus maximinoi and Pinus tecunumanii in a tropical environment and artificial screening of high- elevation P. tecunumanii for resistance to Fusarium circinatum. MSc Thesis. North Carolina State University, Raleigh, NC USA. 103 pp.
Isaza N, 2009. Promoción de floración en huertos semilleros de Pinus maximinoi y Pinus tecunumanii en Colombia. Boletín de Noticias CAMCORE para México y Centroamérica 3 (2): 1-4.
Kende H, Zeevaart JAD, 1997. The five "classical" plant hormones. Plant Cell 9: 1197–1210. http://dx.doi.org/10.1105/tpc.9.7.1197
Kong L, von Aderkas P, 2004. Plant growth regulators and cone induction in Pinaceae. University of Victoria, Victoria, BC. Canada. 27 pp.
Kong L, Abrams SR, Owen SJ, Graham H, von Aderkas P, 2008. Phytohormones and their metabolites during long shoot development in Douglas-fir following cone induction by gibberellin injection. Tree Physiol 28: 1357-1364. http://dx.doi.org/10.1093/treephys/28.9.1357
Kong L, von Aderkas P, Owen SJ, Jaquish B, Woods J, Abrams SR, 2012. Effects of stem girdling on cone yield and endogenous phytohormones and metabolites in developing long shoots of Douglas-fir (Pseudotsuga menziesii). New For 43: 491-503. http://dx.doi.org/10.1007/s11056-011-9294-4
Kong L, von Aderkas P, Zaharia LI, 2015. Effects of exogenously applied gibberellins and thidiazuron on phytohormone profiles of long-shoot buds and cone gender determination in lodgepole pine. J Plant Growth Regul, June 2015.
Longman KA, 1982. Effects of gibberellin, clone and environment on cone initiation, shoot growth and branching in Pinus contorta. Ann Bot 50: 247–257.
Luukkanen O, Johansson S, 1980. Effect of exogenous gibberellin on flowering in Pinus sylvestris grafts. Physiol Plant 50: 365-370. http://dx.doi.org/10.1111/j.1399-3054.1980.tb04114.x
Malabadi RB, van Staden J, 2005. Somatic embryogenesis from vegetative shoot apices of mature trees of Pinus patula. Tree Physiol 25: 11-16. http://dx.doi.org/10.1093/treephys/25.1.11
Meilan R, 1997. Floral induction in woody angiosperms. New For 14: 179-202. http://dx.doi.org/10.1023/A:1006560603966
Morales-González E, López-Upton J, Vargas-Hernández JJ, Ramírez-Herrera C, Gil-Muñoz A, 2013. Pinus patula genetic parameters in a progeny test at two altitudes. Rev Fitotec Mex 36(2): 155-162.
Muñoz-Gutiérrez L, Vargas-Hernández JJ, López-Upton J, Gutiérrez-Rangel N, 2010. Induction of reproductive structures in Pseu¬dotsuga menziesii. Agrociencia 44: 835-847.
Owens JN, 1985. Interaction between gibberellin A4/7 and root-pruning on the reproductive and vegetative processes in Douglas-fir. III. Effects on anatomy of shoot elongation and terminal bud development. Can J For Res 15: 354–364. http://dx.doi.org/10.1139/x85-057
Owens JN, 1995. Constraints to seed production: temperate and tropical forest trees. Tree Physiol 15: 477-484. http://dx.doi.org/10.1093/treephys/15.7-8.477
Owens JN, Chandler LM, Bennett JS, Crowder TJ, 2001. Cone enhancement in Abies amabilis using GA4/7, fertilizer, girdling and tenting. For Ecol Manage 154: 227-236.
Peng Z, Jun-feng F, Shuo-xin Z, Zhong-lian H, Pei-hua Y, Zhen-hua M, Woeste KE, 2011. Effects of gibberellin A4/7, 6-benzylaminopurine and chlormequat chloride on the number of male and female strobili and immature cones in Chinese pine (Pinus tabuliformis) with foliar sprays. J For Res 22: 353-359. http://dx.doi.org/10.1007/s11676-011-0179-3
Perry JS, 1991. The Pines of Mexico and Central America. Timber Press, Inc. USA. 231 pp.
Pharis RP, Weber JE, Ross SD. 1987. The promotion of flowering in forest trees by gibberellin A4+7 and cultural treatments: a review of the possible mechanisms. For Ecol Manage 19: 65-84.
Philipson JJ, 1990. Prospects for enhancing flowering of conifers and broadleaves of potential silvicultural importance in Britain. Forestry 63: 223-240. http://dx.doi.org/10.1093/forestry/63.3.223
Philipson JJ, 1992. Optimal conditions for inducing coning of container-grown Picea sitchensis grafts: effects of applying different quantities of GA4/7, timing and duration of heat and drought treatment, and girdling. For Ecol Manage 53: 39-52.
Philipson JJ, 1996. Effects of girdling and gibberellins A4/7 on flowering of European and Japanese larch grafts in an outdoor clone bank. Can J For Res 26: 355-359. http://dx.doi.org/10.1139/x26-039
Ross SD, 1991. Promotion of flowering in western larch by girdling and gibberellins A4/7 and recommendations for selection and treatment of seed trees. British Columbia. Res note no. 105. 13 pp.
Ross SD, 1992. Promotion of flowering in Engelmann-white spruce seed orchards by GA4/7 stem injection: effects of site, clonal fecundity, girdling and NAA, and treatment carry-over effects. For Ecol Manage 50: 43-55.
SAS Institute, 1998. SAS User´s Guide: Statistics. Release 6.03 Ed. SAS Insti¬tute, Inc. Cary, NC. 1028 pp.
Salaya-Domínguez JM, López-Upton J, Vargas-Hernández JJ, 2012. Genetic and environmental variation in two progeny tests of Pinus patula. Agrociencia 46 (5): 519-534.
Siregar IZ, Sweet GB, 1996. Optimal timing of gibberellin A4/7 application to increase female strobilus numbers in a Pinus radiata seed orchard. N Z J For Sci 26: 339-347.
Sirikul W, Luukkanen O, 1987. Promotion of flowering using exogenous plant hormones in tropical pines. For Ecol Manage 19: 155-161.
Velázquez MA, Angeles PG, Llanderal OT, Román JAR, Reyes HV, 2004. Monografía de Pinus patula. Comisión Nacional Forestal-Colegio de Postgraduados, México. 124 pp.
von Aderkas P, Kong L, Abrams S, Zaharia I, Owens S, Porter B, 2004. Flower induction methods for lodgepole pine and Douglas-fir. Centre for Forest Biology, University of Victoria. 59 pp.
Wakushima S, 2004. Promotion of female strobili flowering and seed production in two Japanese pine species by 6-Benzylaminopurine (BAP) paste application in a field seed orchard. J Plant Growth Regul 23: 135-145. http://dx.doi.org/10.1007/s00344-004-0050-2
Wakushima S, Yoshioka H, Sakurai N, 1997. Promotion of lateral female strobili production in Pinus densiflora by cytokinin application at a specific stage. J For Res 2: 51–57. http://dx.doi.org/10.1007/BF02348263
Wheeler NC, Bramlett DL, 1991. Flower stimulation treatments in a loblolly pine seed orchard. S J Appl For 15: 44-50.
Wormald PJ, 1975. Pinus patula. Tropical Forestry Paper No. 7. Tropical Forestry Institute, Commonwealth Forestry Institute, Oxford, England. 172 pp.
Zar J, 1996. Biostatistical Analysis. Prentice-Hall. New Jersey. USA. 662 pp.
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