Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores

Aranzazu Gomez-Garay, Beatriz Pintos, José Antonio Manzanera, Carmen Prada, Luisa Martin, José María Gabriel y Galan


Aim of study: The effect of cerium oxide engineered nanoparticles on the spore germination of the fern. Asplenium adiantum-nigrum.

Area of study: France, Britanny Region, Finistére Department, Plougonvelin, in rocks near the sea.

Material and methods: Asplenium spores were cultured in vitro on agar medium with Nano-CeO2 (less than 25 nm particle size) and bulk-CeO2. The addition of each nano- and bulk particles ranged from 0 to 3000 mg L-1. Observations on rhizoidal and prothallial cells during first stages of gametophyte development were made. The No-Observed-Adverse-Effect concentration (NOAEC) and Lowest-Observed-Adverse-Effect-Concentration (LOEC) values for spore germination rate data were analyzed.

 Main results: Germination was speeded up by 100 to 2000 mg L-1 nanoceria, while bulk cerium oxide had the same effect for 500 to 200 mg L-1 concentrations. Present results showed cellular damage in the protonema while rhizoid cells seemed not to be affected, as growth and membrane integrity remained.

Research highlights: Both nanosized and bulk cerium oxide are toxic for the fern Asplenium adiantum-nigrum, although diverse toxicity patterns were shown for both materials. Diverse toxic effects have been observed: chloroplast membrane damage and lysis, cell wall and membrane disruption which leads to cell lysis; and alterations in morphology and development.

Keywords: Nanoparticles; rhizoid; prothallus; chloroplast; fern.


Nanoparticles; rhizoid; prothallus; chloroplast; fern.

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DOI: 10.5424/fs/2016253-09294