In vitro effects of copper nanoparticles on plant pathogens, beneficial microbes and crop plants

Susanta Banik, Alejandro Pérez-de-Luque

Abstract


Copper-based chemicals are effectively used as antimicrobials in agriculture. However, with respect to its nanoparticulate form there has been limited number of studies. In this investigation, in vitro tests on effect of copper nanoparticles (CuNPs) against plant pathogenic fungi, oomycete, bacteria, beneficial microbes Trichoderma harzianum and Rhizobium spp., and wheat seeds were conducted. Integration of CuNPs with non-nano copper like copper oxychloride (CoC) at 50 mg/L concentration each recorded 76% growth inhibition of the oomycete Phytophthora cinnamomi in vitro compared to the control. CuNPs also showed synergistic inhibitory effect with CoC on mycelial growth and sporulation of A. alternata. Pseudomonas syringae was inhibited at 200 mg/L of CuNPs. CuNPs were not significantly biocidal against Rhizobium spp. and Trichoderma harzianum compared to CoC. Evaluation of the effect of CuNP on wheat revealed that rate of germination of wheat seeds was higher in presence of CuNPs and CoC compared to control. Germination vigor index, root length, shoot dry weight and seed metabolic efficiency of wheat were negatively affected. At low concentration, CuNPs promoted the growth of the plant pathogenic fungi Botrytis fabae, Fusarium oxysporum f.sp. ciceris, F.oxysporum f.sp. melonis, Alternaria alternate and P. syringae, and sporulation of T. harzianum. Synergistic effect of CuNPs and CoC in inhibiting P. cinnamomi offers a possibility of developing new fungicide formulation for better control of the oomycetes. Non-biocidal effect of CuNPs against beneficial microbes indicates its potential use in the agri-ecosystem.


Keywords


Phytophthora cinnamomi; Trichoderma; Pseudomonas; Rhizobium; wheat

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References


Al Abboud MA, Alawlaqi MM, 2011.Bio-uptake of copper and their impact on fungal fatty acids. Aust J Basic Appl Sci 5: 283-290.

Alexopoulos CJ, Mims CW, Blackwell M, 2002. Introductory Mycology, 4th ed. Wiley India, New Delhi, 880 pp.

Anand P, Isar J, Saran S, Saxena RK, 2006. Bioaccumulation of copper by Trichoderma viride. Bioresour Technol 97: 1018-1025. https://doi.org/10.1016/j.biortech.2005.04.046

Arduini I, Godbold DL, Onnis A, 1995. Influence of copper on root growth and morphology of Pinus pinea L. and Pinus pinaster Ait. seedlings. Tree Physiol 15: 411-415. https://doi.org/10.1093/treephys/15.6.411

Avery SV, Howlett NG, Radice S, 1996. Copper toxicity towards Saccharomyces cerevisiae: dependence on plasma membrane fatty acid composition. Appl Environ Microbiol 62 (11): 3960-3966.

Banik S, Sharma P, 2011. Plant Pathology in the era of nanotechnology. Ind Phytopathol 64 (2): 120-127.

Cioffi N, Torsi L, Ditaranto N, Tantillo G, Ghibelli L, Sabbatini L, Bleve-Zacheo T, D'Alessio M, Zambonin PG, Traversa E, 2005. Copper nanoparticle/polymer composites with antifungal and bacteriostatic properties. Chem Mater 17: 5255-5262. https://doi.org/10.1021/cm0505244

Copeland LO, 1976. Principles of seed science and technology. Burgress Pub. Com., Minneapolis, MN, USA.

Denkhaus E, Salnikow K, 2002. Nickel essentiality, toxicity, and carcinogenicity. Crit Rev in Oncol/Hematol 42: 35-56. https://doi.org/10.1016/S1040-8428(01)00214-1

Dorn B, Musa T, Krebs H, Fried PM, Forrer HR, 2007. Control of late blight in organic potato production: evaluation of copper-free preparations under field, growth chamber and laboratory conditions. Eur J Plant Pathol 119: 217-240. https://doi.org/10.1007/s10658-007-9166-0

Englander CM, Corden ME, 1971. Stimulation of mycelial growth of Endothia parasitica by heavy metals. Appl Microbiol 22: 1012-1016.

Grass G, Rensing C, Solioz M, 2011.Metallic copper as an antimicrobial surface. Appl Environ Microbiol 77 (5): 1541-1547. https://doi.org/10.1128/AEM.02766-10

Guzman M, Dile J, Godet S, 2009. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biomol Eng 2 (3): 1-8.

Hong R, Kang TY, Michels CA, Gadura N, 2012. Membrane lipid peroxidation in copper alloy-mediated contact killing of Escherichia coli. Appl Environ Microbiol 78 (6): 1776-1784. https://doi.org/10.1128/AEM.07068-11

Hoshino N, Kimura T, Yamaji A, Ando T, 1999. Damage to the cytoplasmic membrane of Escherichia coli by catechin-copper (II) complexes. Free Radical Biol Med 27 (11-12): 1245-1250. https://doi.org/10.1016/S0891-5849(99)00157-4

Hoshino N, Kimura T, Hayakawa F, Yamaji A, Ando T, 2000. Bactericidal activity of catechin-copper (II) complexes against Staphylococcus aureus compared with Escherichia coli. Lett Appl Microbiol 31 (3): 213-217. https://doi.org/10.1046/j.1365-2672.2000.00800.x

Janse JD, 2005. Phytobacteriology: Principles and Practices. CAB Int, Wallingford, UK, 360 pp. https://doi.org/10.1079/9781845930257.0000

Jia B, Mei Y, Cheng L, Zhou J, Zhang L, 2012. Preparation of copper nanoparticles coated cellulose films with antibacterial properties through one-step reduction. ACS Appl Mater Interfaces 4: 2897-2902. https://doi.org/10.1021/am3007609

Jiang W, Liu D, Liu X, 2001. Effects of copper on root growth, cell division, and nucleolus of Zea mays. Biol Plant 44: 105-109. https://doi.org/10.1023/A:1017982607493

Johnson GF, 1935.The early history of copper fungicides. Agric Hist 9: 67-79.

Krishnasamy V, Seshu DV, 1990. Germination after accelerated aging and associated characters in rice varieties. Seed Sci Technol 18: 353-359.

Laguerre G, Courde L, Nouaïm L, Lamy I, Revellin C, Breuil MC, Chaussod R, 2006. Response of rhizobial populations to moderate copper stress applied to an agricultural soil. Microb Ecol 52: 426-435. https://doi.org/10.1007/s00248-006-9081-5

Lamsal K, Kim SW, Jung JH, Kim YS, Kim KS, Lee YS, 2011.Application of silver nanoparticles for the control of Colletotrichum sp. in vitro and pepper anthracnose disease in the field. Mycobiology 39 (3): 194-199. https://doi.org/10.5941/MYCO.2011.39.3.194

Large EC, 1943. Control of potato blight (Phytophthora infestans) by spraying with suspensions of metallic copper. Nature 151: 80-81. https://doi.org/10.1038/151080b0

Millardet A, 1886. Traitement du mildiouet du rot par le mélange de chaux et sulfate de cuivre. Masson, Feretetfils, Paris.

Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ, 2005.The bactericidal effect of silver nanoparticles. Nanotechnology 16: 2346-2353. https://doi.org/10.1088/0957-4484/16/10/059

Pérez-de-Luque A, Cifuentes Z, Beckstead JA, Sillero JC, Ávila C, Rubio J, Ryan RO, 2011. Effect of amphotericin B nanodisks on plant fungal diseases. Pest Manage Sci 68: 67-74. https://doi.org/10.1002/ps.2222

Pérez-de-Luque A, Hermosín C, 2013.Nanotechnology and its use in agriculture. In: Bio-nanotechnology: a revolution in food, biomedical and health sciences; Bagchi D, Bagchi M, Moriyama H, Shahidi F (eds.). pp: 383-398. Blackwell Publ Ltd., Oxford(UK). https://doi.org/10.1002/9781118451915.ch20

Raffi M, Mehrwan S, Bhatti TM, Akhter JI, Hameed A, Yawar W, UlHasan MM, 2010. Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli. Ann Microbiol 60 (1): 75-80. https://doi.org/10.1007/s13213-010-0015-6

Rao DG, Sinha SK, 1993. Efficiency of mobilization of seed reserves in sorghum hybrids and their parents as influenced by temperature. Seed Res 21 (2): 97-100.

Ren G, Hu D, Cheng EWC, Vargas-Reus MA, Reip P, Allaker RP, 2009.Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 33: 587-590. https://doi.org/10.1016/j.ijantimicag.2008.12.004

Shobha G, Moses V, Ananda S, 2014. Biological synthesis of copper nanoparticles and its impact-a review. Int J Pharm Sci Invent 3 (8): 28-38.

Sikder S, Paul NK, 2010. Study of influence of temperature regimes on germination characteristics and seed reserves mobilization in wheat. Afr J Plant Sci 4 (10): 401-408.

Singh A, Singh NB, Hussain I, Singh H, Singh SC, 2015. Plant-nanoparticle interaction: an approach to improve agricultural practices and plant productivity. Int J Pharm Sci Invent 4 (8): 25-40.

Yoon K, Byeon JH, Park J, Hwang J, 2007. Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and CuNPs. Sci Total Environ 373: 572-575. https://doi.org/10.1016/j.scitotenv.2006.11.007




DOI: 10.5424/sjar/2017152-10305