Performance, carcass and ruminal fermentation characteristics of heifers fed concentrates differing in energy level and cereal type (corn vs. wheat)

Coral Carrasco, María D. Carro, Alfonso Fuentetaja, Pedro Medel


A total of 144 beef heifers (218 ± 26.4 kg body weight) were housed in 24 pens (6 animals each) and used in a 168-day feedlot study to evaluate the influence of cereal type and energy level on performance, carcass quality and ruminal fermentation. Four concentrates were formulated according to a 2×2 factorial arrangement of treatments, with two energy levels (1,452 vs. 1,700 kcal net energy/kg) and two main cereals (wheat vs. corn). Concentrate and straw were offered ad libitum. Concentrate intake and body weight were recorded on days 42, 84, 126 and 168. Ruminal fluid was obtained by ruminocentesis from 3 heifers per pen on days 1, 84 and 168; and carcass weight, classification and yield, were determined in the same animals. Heifers fed high-energy diets had lower intake (6.97 vs. 7.29 kg fresh matter/d; p=0.011), and lower concentrate to gain ratio (5.15 vs. 5.66 kg/kg; p=0.002) than those fed low energy concentrates, and tended (p=0.069) to be heavier along the time. Neither carcass yield and classification, nor ruminal pH, volatile fatty acids nor NH3-N concentrations were affected (p>0.050) by energy level. Total volatile fatty acids concentration tended (p=0.070) to be greater in heifers fed corn-based than wheat-based concentrates. No energy level x cereal type interactions were observed. These results indicate that high energy concentrates decreased feed intake and feed conversion but had minor effects on carcass performance. Cereal type had no effects on performance and ruminal fermentation and no interactions between cereal type and energy were detected.


beef cattle; ruminal pH; volatile fatty acids; in vitro fermentation

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AOAC, 1999. Official Methods of Analysis, 16th ed. Assoc. Off. Anal. Chem. Int., Gaithersburg, MD, USA.

Bacha F, 1991. Efecto de las características fisicoquímicas de los cereales y subproductos de molinería sobre la degradabilidad de las materias nitrogenadas. Tesis Doctoral. Universidad Politécnica de Madrid.

Beauchemin K, Penner G, 2009. New developments in understanding ruminal acidosis in dairy cows. Tri-State Dairy Nutrition Conference, 21-22 Apr, pp: 1-12.

Bock BJ, Brandt RT, Harmon DL, Andersons SJ, Elliott JK, Avery TB, 1991. Mixtures of wheat and high-moisture corn in finishing diets: feedlot performance and in situ rate of starch digestion in steers. J Anim Sci 69: 2703-2710.

BOE, 2013. Royal Decree 53/2013, of 1 February, establishing basic standards for the protection of animals used in experiments and for other scientific purposes, including education. Boletín Oficial del Estado, Spain, No. 34, 08/02/2013.

Calsamiglia S, Blanch M, Ferret A, Moya D, 2012. Is subacute ruminal acidosis a pH related problem? Causes and tools for its control. Anim Feed Sci Technol 172: 42-50.

Carrasco C, Medel P, Fuentetaja A, Carro MD, 2012. Effect of malate form (acid or disodium/calcium salt) supplementation on performance, ruminal parameters and blood metabolites of feedlot cattle. Anim Feed Sci Technol 176: 140-149.

Carro MD, López S, Valdés C, Ovejero FJ, 1999. Effect of DL-malate on mixed ruminal microorganism fermentation using the rumen simulation technique (RUSITEC). Anim Feed Sci Technol 79: 279-288.

Corona L, Owens FN, Zinn RA, 2006. Impact of corn vitreousness and processing on site and extent of digestion by feedlot cattle. J Anim Sci 84: 3020-3031.

Danscher AM, Li S, Andersen PH, Khafipour E, Kristensen NB, Plaizier JC, 2015. Indicators of induced subacute ruminal acidosis (SARA) in Danish Holstein cows. Acta Vet Scand 57 (1): 39.

de Blas C, Mateos GG, García-Rebollar P, 2010. Tablas FEDNA de composición y valor nutritivo de alimentos para la fabricación de piensos compuestos, 3th rev. ed. Fundación Española para el Desarrollo de la Nutrición Animal, Madrid.

Devant M, Ferret A, Gasa J, Calsamiglia S, Casals R, 2000. Effects of protein concentration and degradability on performance, ruminal fermentation, and nitrogen metabolism in rapidly growing heifers fed high-concentrate diets from 100 to 230 kg body weight. J Anim Sci 78: 1667-1676.

Devant M, Bach A, Solé A, Quintana B, Verdú M, 2015. La importancia del manejo alimentario del ternero. I Jornada Fedna-Anembe, 25 Aniversario Nutrición de Rumiantes.

Duffield T, Plaizier JC, Fairfield A, Bagg R, Vessie G, Dick P, Wilson J, Aramini J, McBride B, 2004. Comparison of techniques for measurement of rumen pH in lactating dairy cows. J Dairy Sci 87: 59-66.

Ferraretto LF, Crump PM, Shaver RD, 2013. Effect of cereal grain type and corn grain harvesting and processing methods on intake, digestion, and milk production by dairy cows through a meta-analysis. J Dairy Sci 96: 533-550.

Fulton WR, Klopfenstein TJ, Britton RA, 1979. Adaptation to high concentrate diets by beef cattle. I. Adaptation to corn and wheat diets. J Anim Sci 49: 775-784.

Gelberg HB, 2016. Alimentary system and the peritoneum, omentun, mesentery, and peritoneal cavity. In: Pathologic basis of veterinary disease expert consult, Chapter 7; Zachary JF, McGavin MD (eds.), pp: 324-411. Elsevier Health Sci, St. Louis. USA.

Gimeno A, Dieste A, Al-Alami A, de-Vega A, Castrillo C, Fondevilla M, 2014. Effect of feed presentation form on the intake pattern, productive traits and rumen pH of beef cattle fed high concentrate diets. Span J Agric Res 12: 1105-1109.

Goering MK, Van-Soest PJ, 1970. Forage fiber analysis (apparatus, reagents, procedures and some applications). Agricultural Handbook 379. Agr Res Serv USDA, Washington DC, USA. 24 pp.

González LA, Ferret A, Manteca X, Ruiz-de-la-Torre JL, Calsamiglia S, Devant M, Bach A, 2008a. Performance, behavior, and welfare of Friesian eifers housed in pens with two, four, and eight individuals per concentrate feeding place. J Anim Sci 86: 1446-1458.

González LA, Ferret A, Manteca X, Ruiz-de-la-Torre JL, Calsamiglia S, Devant M, Bach A, 2008b. Effect of the number of concentrate feeding places per pen on performance, behavior, and welfare indicators of Friesian calves during the first month after arrival at the feedlot. J Anim Sci 86: 419-431.

Gozho GN, Mutsvangwa T, 2008. Influence of carbohydrate source on ruminal fermentation characteristics, performance, and microbial protein synthesis in dairy cows. J Dairy Sci 91: 2726-2735.

Iraira SP, Madruga A, Pérez-Juan M, Ruiz-de-la-Torre JL, Rodríguez-Prado M, Calsamiglia S, Ferret A, 2015. Performance, behaviour and meat quality of beef heifers fed concentrate and straw offered as total mixed ration or free-choice. Span J Agric Res 13: e0610.

Khan MA, Lee HJ, Lee WS, Kim HS, Kim HB, Park SB, Baek KS, Ha JK, Choi YJ, 2008. Starch source evaluation in calf starter: II. Ruminal parameters, rumen development, nutrient digestibilities, and nitrogen utilization in Holstein calves. J Dairy Sci 91: 1140-1149.

Krehbiel CR, Cranston JJ, McCurdy MP, 2006. An upper limit for caloric density of finishing diets. J Anim Sci 84: E34-E49.

Kreikemeier KK, Stock RA, Briok DR, Brim RA, 1987. Feeding combinations of dry corn and wheat to finishing lambs and cattle. J Anim Sci 65: 1647-1654.

Li L, Zhu Y, Wang X, He Y, Cao B, 2014. Effects of different dietary energy and protein levels and sex on growth performance, carcass characteristics and meat quality of F1 Angus x Chinese Xiangxi yellow cattle. J Anim Sci Biotechnol 5: 21.

Liu Y, Zhao H, Liu X, You W, Cheng H, Wan F, Zhang X, 2016. Substitution of wheat for corn in beef cattle diets: digestibility, digestive enzyme activities, serum metabolite contents and ruminal fermentation. Asian Australas J Anim Sci 29: 1424-1431.

Maia MR, Chaudhary LC, Figueres L, Wallace RJ, 2007. Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen. A van Leeuw J Microb 91: 303-314.

Mateos I, Ranilla MJ, Tejido ML, Saro C, Kamel C, Carro MD, 2013. The influence of diet on the effectiveness of garlic oil and cinnamaldehyde to manipulate in vitro ruminal fermentation and methane production. Anim Prod Sci 53: 299-307.

Mateos I, Ranilla MJ, Saro C, Carro MD, 2015. Comparison of fermentation characteristics and bacterial diversity in the rumen of sheep and batch cultures of rumen microorganisms. J Agric Sci 153: 1097-1106.

McAllister TA, Phillippe RC, Rode LM, Cheng KJ, 1993. Effect of the protein matrix on the digestion of cereal grains by ruminal microorganisms. J Anim Sci 71: 205-212.

McAllister TA, Gibb DJ, Beauchemin KA, Wang Y, 2006. Starch type and ruminal digestion. In: Cattle grain processing symposium, pp: 30-41. Tulsa, Oklahoma State Univ, USA.

Muller LD, 1987. Branched chain fatty acids (isoacids) and valeric acid for ruminants. Prof Anim Sci 3: 9-12.

Nockels CF, Kintner LD, Pfander WH, 1966. Influence of ration on morphology, histology, and trace mineral content of sheep rumen papillae. J Dairy Sci 49: 1068-1074.

NRC, 2000. Nutrient requirements of beef cattle, 7th rev. ed. National Research Council, Natl Acad Press, Washington, DC, USA.

OJEU, 2006. Council Regulation (EC) No 1183/2006 of 24 July 2006 concerning the Community scale for the classification of carcasses of adult bovine animals. Official Journal of the European Union L 214, 4.8.2006, p. 1-6.

Oltjen RR, Putnam PA, Williams EE, Davis RE, 1966. Wheat versus corn in all-concentrate cattle rations. J Anim Sci 25: 1000-1004.

Owens FN, Secrist DS, Hill WH, Gill DR, 1997. The effect of grain source and grain processing on performance of feedlot cattle: A review. J Anim Sci 75: 868-879.

Philippeau C, Martin C, Michalet-Doreau B, 1999. Influence of grain source on ruminal characteristics and rate, site, and extent of digestion in beef steers. J Anim Sci 77:1587-1596.

Rooney LW, Pflugfelder RL, 1986. Factors affecting starch digestibility with special emphasis on sorghum and corn. J Anim Sci 63: 1607-1623.

Taylor KACC, 1996. A simple colorimetric assay for muramic acid and lactic acid. Appl Biochem Biotechnol 56: 49-58.

Tejido ML, Ranilla MJ, García-Martínez R, Carro MD, 2005. In vitro microbial growth and rumen fermentation of different diets as affected by the addition of disodium malate. Anim Sci 81: 31-38.

Van Soest PJ, Robertson JB, Lewis BA, 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74: 3583-3597.

Verdú M, Bach A, Devant M, 2015. Effect of concentrate feeder design on performance, eating and animal behavior, welfare, ruminal health, and carcass quality in Holstein bulls fed high-concentrate diets. J Anim Sci 93: 3018-3033.

Weatherburn MW, 1967. Phenol hypoclorite reaction for determination of ammonia. Anal Chem Am Chem Soc 89: 971-974.

Yang WZ, Xu L, Zhao YL, Chen LY, McAllister TA, 2014. Impact of hard vs. soft wheat and monensin level on rumen acidosis in feedlot heifers. J Anim Sci 92: 5088-5098.

DOI: 10.5424/sjar/2017154-11230