Coupling autotrophic in vitro plant cultivation system to scanning electron microscope to study plant-fungal interactions

  • N. De Jaeger Earth and Life Institute. Mycology. Université catholique de Louvain. 3 Place Croix du Sud. 1348 Louvain la Neuve
  • C. Decock Earth and Life Institute. Mycology. Mycothèque de l’Université catholique de Louvain (BCCM/MUCL**). 3 Place Croix du Sud. 1348 Louvain la Neuve
  • S. Declerck Earth and Life Institute. Mycology. Université catholique de Louvain. 3 Place Croix du Sud. 1348 Louvain la Neuve
  • I. E. de la Providencia Earth and Life Institute. Mycology. Université catholique de Louvain. 3 Place Croix du Sud. 1348 Louvain la Neuve
DOI: https://doi.org/10.5424/sjar/201008S1-1224
Keywords: arbuscular mycorrhizal fungi, autotrophic culture system, potato, Rhizoctonia, scanning electron microscope, Trichoderma

Abstract

The interactions of plants with pathogens and beneficial micro-organisms have been seldom compared on the same host and under strict controlled autotrophic in vitro culture conditions. Here, the life cycle of two plant beneficial (Glomus sp. MUCL 41833 and Trichoderma harzianum) and one plant pathogen (Rhizoctonia solani) fungi were described on potato (Solanum tuberosum) plantlets under autotrophic in vitro culture conditions using video camera imaging and the scanning electron microscope (SEM). (i) The colony developmental pattern of the extraradical mycelium within the substrate, (ii) the reproduction structures and (iii) the three-dimensional spatial arrangements of the fungal hyphae within the potato root cells were successfully visualized, monitored and described. The combination of the autotrophic in vitro culture system and SEM represent a powerful tool for improving our knowledge on the dynamics of plant-fungal interactions.

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Published
2010-07-22
How to Cite
De Jaeger, N., Decock, C., Declerck, S., & de la Providencia, I. E. (2010). Coupling autotrophic in vitro plant cultivation system to scanning electron microscope to study plant-fungal interactions. Spanish Journal of Agricultural Research, 8(S1), 69-78. https://doi.org/10.5424/sjar/201008S1-1224
Issue
Vol. 8 (2010): Special issue (S1). Dedicated to cost action 870.
Section
Invisible

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