Arbuscular mycorrhizal fungi associated with psammophilic vegetation in Mediterranean coastal sand dunes

Abstract

This study was conducted in order to characterize the natural arbuscular mycorrhizal (AM) biodiversity from Mediterranean sand dune ecosystems and to protect in a collection this biodiversity. The occurrence of AM fungi associated with sand dune plant species in three Mediterranean locations on the north-eastern coast of Spain was examined in one well preserved coastal sand dune and in two embrionary dunes recently protected from public access. Traditional taxonomy and molecular techniques were used to identify the AM fungal species present in these ecosystems. The species identified and isolated were: Scutellospora persica (Koske and Walker) Walker & Sanders, Glomus ambisporum Smith & Schenck, Glomus diaphanum Morton & Walker, Glomus clarum Nicolson & Schenck, Glomus intraradices Schenck & Smith, Glomus microaggregatum Koske, Gemma & Olexia and Gigaspora margarita Becker & Hall. Spores of Glomus were the most abundant in the direct soil extraction samples. The molecular analysis indicates that the most abundant fungi forming AM in the roots belonged to the Gigasporaceae group followed by fungi of Glomus group A and Glomus group B. The highest diversity of fungi and abundance of the AM fungal spores was found in the well preserved and undisturbed dune systems.