Short communication: Identification of cultivated and wild Vaccinium species grown in Portugal

Márcia Carvalho, Manuela Matos, Valdemar Carnide

Abstract


Vaccinium crops offer a variety of benefits for human health due their high levels of antioxidants. Genetic diversity between two Vaccinium species (sixteen cultivars of Vaccinium corymbosum and three wild populations of Vaccinium myrtillus) were evaluated using Inter Simple Sequence Repeat (ISSR) markers. In V. corymbosum 74 polymorphic markers corresponding to 83.2% of polymorphism were obtained while in V. myrtillus only four polymorphic markers corresponding to 83.2% and 10.6% of polymorphism were observed. The dendrogram obtained showed a clear division into two distinct groups corresponding to the two analyzed species. V. corymbosum group is divided in different sub-clusters based on cultivars pedigree relationships. Twenty-eight specific bands were detected in total; 6 for V. corymbosum and 22 for V. myrtillus. Results allowed the selection of five primers due it potential to detect specific bands in the two species. These markers could be useful for identifying species and cultivars and consequently help in the management of germplasm collections and in breeding programs.


Keywords


blueberry; bilberry; genetic diversity; differentiation; molecular marker

Full Text:

PDF HTML XML

References


Albert T, Raspé O, Jacquemart AL, 2004. Clonal diversity and genetic structure in Vaccinium myrtillus populations from different habitats. Belg J Bot 137 (2): 155-162.

Boches P, Bassil NV, Rowland L., 2006. Genetic diversity in the highbush blueberry evaluated with microsatellite markers. J Am Soc Hortic Sci 131 (5): 674-686.

Carvalho M, Matos M, Carnide V, 2014. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort Sci (Prague) 41: 175-184. https://doi.org/10.17221/21/2014-HORTSCI

Debnath SC, 2007a. Inter simple sequence repeat (ISSR) to assess genetic diversity within a collection of wild lingonberry (Vaccinium vitis-idaea L.) clones. Can J Plant Sci 87 (2): 337-344. https://doi.org/10.4141/P06-059

Debnath SC, 2007b. Inter simple sequence repeat (ISSR) markers and pedigree information to assess genetic diversity and relatedness within raspberry genotypes. Intern J Fruit Sci 4 (4): 1-17. https://doi.org/10.1080/15538360802003159

Debnath SC, 2009. Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium. Nord J Bot 27 (2): 141-148. https://doi.org/10.1111/j.1756-1051.2009.00402.x

Garriga M, Parra PA, Caligari PDS, Retamales JB, Carrasco BA, Lobos GA, García-Gonzáles R, 2013. Application of inter-simple sequence repeats relative to simples sequence repeats as a molecular marker system for indexing blueberry cultivars. Can J Plant Sci 93: 913-921. https://doi.org/10.4141/cjps2013-057

Hancock J, 2009. Highbush blueberry breeding. Latvian J Agron 12: 35-38.

Kumar P, Gupta VK, Misra AK, Modi DR, Pandey BK, 2009. Potential of molecular markers in plant biotechnology. Plants Omics J 2: 141-162.

Matos M, Pinto-Carnide O, Benito C, 2001. Phylogenetic relationships among Portuguese rye based on isozyme, RAPD and ISSR markers. Hereditas 134 (3): 229-236. https://doi.org/10.1111/j.1601-5223.2001.00229.x

Prevost A, Wilkinson MJ, 1999. A new system of comparing PCR primers applied to ISSR fingerprint of potato cultivars. Theor Appl Genet 98 (1): 107-112. https://doi.org/10.1007/s001220051046

Roldán-Ruiz I, Dendauw J, Van Bockstaele E, Depicker A, De Loose M, 2000. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breeding 6: 125-134. https://doi.org/10.1023/A:1009680614564

Ross S, Castillo A, 2009. Mass propagation of Vaccinium corymbosum in bioreactors. Agrociencia Uruguay XIII (2): 1-8.

Rowland LJ, Alkharouf N, Darwish O, Ogden EL, Polashock JJ, Bassil NV, Main D, 2012. Generation and analysis of blueberry transcriptome sequences from leaves, developing fruit, and flower buds from cold acclimation through deacclimation. BMC Plant Biol 12: 46. https://doi.org/10.1186/1471-2229-12-46

Sorkheh K, Shiran B, Gradziel TM, Epperson BK, Martinez-Gomez P, Asadi E, 2007. Amplified fragment length polymorphism as a tool for molecular characterization of almond germplasm: genetic diversity among cultivated genotypes and related wild species of almond, and its relationships with agronomic traits. Euphytica 156: 327-344. https://doi.org/10.1007/s10681-007-9382-x

Zietkiewicz E, Rafalski A, Labuda D, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chains reaction amplification. Genomics 20: 176-183. https://doi.org/10.1006/geno.1994.1151

Zifkin M, Jin A, Ozga JA, Zaharia I, Schemthaner JP, Gesells A, Abrams SR, Kennedy JA, Constabel CP, 2012. Gene expression and metabolite profiling of developing highbush blueberry fruit indicates transcriptional regulation of flavonoid metabolism and activation of abscisic acid metabolism. Plant Physiol 158: 200-224. https://doi.org/10.1104/pp.111.180950




DOI: 10.5424/sjar/2018163-12502