Short communication: The percentage of egg yolk in the freezing media affects mouflon (Ovis musimon) epididymal sperm cryosurvival
Abstract
Sperm cryopreservation protocols are not well defined in many wild species such as the mouflon. The aim was to study the effect of different concentrations of EY on mouflon epididymal sperm cryosurvival. Samples were collected by the flushing method and cryopreserved by the conventional freezing technique in straws using a TEST (TES, Tris, glucose) 5% v/v glycerol medium containing either 6% v/v (n=16) or 12% v/v (n=13) clarified EY. The membrane integrity, acrosome integrity, motility, and morphological abnormalities were assessed in fresh and frozen/thawed samples. Fresh sperm quality parameters did not differ between groups except for the acrosome integrity that was lower in the TEST-6% EY than in the TEST-12% EY group (88.9 ± 2.1% vs 94.7 ± 0.8%). Membrane integrity (31.6 ± 4.6% vs 11.6 ± 4.5%), total motility (32.8 ± 4.6% vs 17.2 ± 5.6%), progressive motility (13.3 ± 2.7% vs 6.1 ± 2.9) were higher in frozen-thawed sperm with TEST-6% EY than with TEST-12% EY (p<0.05). Other motility parameters such as curvilinear velocity, straight-line velocity, average path velocity and amplitude of lateral head displacement were also higher (p<0.05) in frozen-thawed sperm with TEST-6% EY. Frozen-thawed acrosome integrity (85.1 ± 3.3% vs 91.9 ± 2.3) and morphological abnormalities (34.0 ± 3.7% vs 29.1 ± 3.6%) did not differ between extenders. In conclusion, high EY concentration had detrimental effects on post-thaw quality parameters, therefore the use of TEST based extender containing 6% EY is recommended for the cryopreservation of mouflon epididymal sperm.
Downloads
References
Álvarez M, Tamayo-Canul J, Martinez-Rodriguez C, Lopez-Uruena E, Gomes-Alves S, Anel L, Martinez-Pastor F, de Paz P, 2012. Specificity of the extender used for freezing ram sperm depends of the spermatozoa source (ejaculate, electroejaculated or epididymis). Anim Reprod Sci 132 (3-4): 145-154. https://doi.org/10.1016/j.anireprosci.2012.05.006
Bergeron A, Crête M-H, Brindle Y, Manjunath P, 2004. Low-density lipoprotein fraction from hen's egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biol Reprod 70 (3): 708-717. https://doi.org/10.1095/biolreprod.103.022996
Cabrera F, Gonzalez F, Batista M, Calero P, Medrano A, Gracia A, 2005. The effect of removal of seminal plasma, egg yolk level and season on sperm freezability of canary buck (Capra hircus). Reprod Domest Anim 40 (3): 191-195. https://doi.org/10.1111/j.1439-0531.2005.00544.x
Cookson AD, Thomas AN, Foulkes JA, 1984. Immunochemical investigation of the interaction of egg-yolk lipoproteins with bovine spermatozoa. J Reprod Fertil 70 (2): 599-604. https://doi.org/10.1530/jrf.0.0700599
Dong Q, VandeVoort CA, 2009. Effect of egg yolk on cryopreservation of rhesus monkey ejaculated and epididymal sperm. J Androl 30 (3): 309-316. https://doi.org/10.2164/jandrol.108.006395
Fernandez-Santos MR, Esteso MC, Montoro V, Soler AJ, Garde JJ, 2006. Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa: Effects of egg yolk, glycerol and cooling rate. Theriogenology 66 (8): 1931-1942. https://doi.org/10.1016/j.theriogenology.2006.05.012
Ferreira VdS, Mello MRBd, Fonseca CEMd, Dias ÁCF, Cardoso JM, Silva RB, Martins Júnior WP, 2014. Effect of seminal plasma and egg yolk concentration on freezability of goat semen. R Bras Zootec 43 (10): 513-518. https://doi.org/10.1590/S1516-35982014001000001
Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH, 2010. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology 73 (4): 480-487. https://doi.org/10.1016/j.theriogenology.2009.10.005
Garde JJ, del Olmo A, Soler AJ, Espeso G, Gomendio M, Roldan ERS, 2008. Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier's gazelle (Gazella cuvieri). Anim Reprod Sci 108 (3-4): 384-401. https://doi.org/10.1016/j.anireprosci.2007.09.010
Holt WV, Abaigar T, Jabbour HN, 1996. Oestrous synchronization, semen preservation and artificial insemination in the Mohor gazelle (Gazella dama mhorr) for the establishment of a genome resource bank programme. Reprod Fertil Dev 8 (8): 1215-1222. https://doi.org/10.1071/RD9961215
Jimenez E, Perez-Marin CC, Vizuete G, Millan Y, Aguera EI, 2013. Effect of different extenders on in vitro characteristics of feline epididymal sperm during cryopreservation. Reprod Domest Anim 48 (4): 665-672. https://doi.org/10.1111/rda.12142
Jones RC, Martin IC, 1973. The effects of dilution, egg yolk and cooling to 5°C on the ultrastructure of ram spermatozoa. J Reprod Fertil 35 (2): 311-320. https://doi.org/10.1530/jrf.0.0350311
Manjunath P, 2012. New insights into the understanding of the mechanism of sperm protection by extender components. Anim Reprod 9 (4): 809-815.
Pradiee J, Esteso MC, Castaño C, Toledano-Díaz A, López-Sebastián A, Guerra R, Santiago-Moreno J, 2017. Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification. Andrologia 49: e12629. https://doi.org/10.1111/and.12629
Rajabi-Toustani R, Roostaei-Ali Mehr M, Motamedi-Mojdehi R, 2014. Effect of different levels of egg yolk on ram sperm coating and preserving at 5 C. Iran J Vet Res 15 (2): 168-171.
Santiago-Moreno J, Toledano-Diaz A, Pulido-Pastor A, Dorado J, Gomez-Brunet A, Lopez-Sebastian A, 2006. Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal spermatozoa. Theriogenology 66 (5): 1219-1226. https://doi.org/10.1016/j.theriogenology.2006.03.031
Santiago-Moreno J, Castano C, Toledano-Diaz A, Esteso MC, Lopez-Sebastian A, Guerra R, Ruiz MJ, Mendoza N, Luna C, Cebrian-Perez JA, et al., 2013. Cryopreservation of aoudad (Ammotragus lervia sahariensis) sperm obtained by transrectal ultrasound-guided massage of the accessory sex glands and electroejaculation. Theriogenology 79 (2): 383-391. https://doi.org/10.1016/j.theriogenology.2012.10.011
Scott TW, Voglmayr JK, Setchell BP, 1967. Lipid composition and metabolism in testicular and ejaculated ram spermatozoa. The Biochem J 102 (2): 456-461. https://doi.org/10.1042/bj1020456
Şen ÇÇ, Tekin K, Akçay E, 2015. Effect of egg yolk and removal of seminal fluid on semen cryopreservation in Norduz goat. Harran Üniv Vet Fak Derg 4 (2): 64-67.
Soler AJ, Esteso MC, Fernandez-Santos MR, Garde JJ, 2005. Characteristics of Iberian red deer (Cervus elaphus hispanicus) spermatozoa cryopreserved after storage at 5 degrees C in the epididymis for several days. Theriogenology 64 (7): 1503-1517. https://doi.org/10.1016/j.theriogenology.2005.03.013
Ustuner B, Gunay U, Nur Z, 2009. Effect of seminal plasma, egg yolk, and season on the freezability of Saanen buck semen. Bull Vet Inst Pulawy 53: 369-374.
Watson PF, 1975. The interaction of egg yolk and ram spermatozoa studied with a fluorescent probe. J Reprod Fertil 42 (1): 105-111. https://doi.org/10.1530/jrf.0.0420105
Watson PF, 1976. Electroejaculation, semen characteristics and semen preservation of the brindled gnu. J Reprod Fertil 47 (1): 123-126. https://doi.org/10.1530/jrf.0.0470123
Watson PF, 1981. The roles of lipid and protein in the protection of ram spermatozoa at 5 degrees C by egg-yolk lipoprotein. J Reprod Fertil 62 (2): 483-492. https://doi.org/10.1530/jrf.0.0620483
Watson PF, Martin IC, 1975. Effects of egg yolk, glycerol and the freezing rate on the viability and acrosomal structures of frozen ram spermatozoa. Aust J Biol Sci 28 (2): 153-159. https://doi.org/10.1071/BI9750153
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.