Foliar application of nitrogenous compounds and elicitors to Tempranillo grapevines: Microbiological implications

Lucía González-Arenzana, Javier Portu, Ana R. Gutiérrez, Pilar Santamaría, Isabel López-Alfaro, Rosa López

Abstract


This study was aimed to analyze the effects of the nitrogen foliar application, and elicitation used on microbial populations of musts. The nitrogenous compounds were raw and treated wastewater from mushroom industry and a commercial yeast derivate; the elicitors were methyl jasmonate, chitosan and one commercial elicitor from a yeast source. Those six products were sprayed as pre-harvest treatment over the leaves of Tempranillo grapevines for improving quality and maturity of grapes. The applications of raw and treated wastewaters from mushroom industry, chitosan and methyl jasmonate elicitation did not cause changes in microbial diversity of must samples. In contrast, spraying commercial yeast derivate products made the must microbiota reach a high richness of species that would be positive in ecological terms. This research succeeded in establishing the impacts of foliar applications in the grapevines on the microbial community, and in any case, negative impacts were observed so that, presumably, these foliar applications can be perfectly implemented as agronomic practice.

Keywords


vine leaves; elicitation; grape; diversity; nitrogen

Full Text:

PDF HTML XML

References


Ancín-Azpilicueta C, Nieto-Rojo R, Gómez-Cordón J, 2013. Effect of foliar urea fertilisation on volatile compounds in Tempranillo wine. J Sci Food Agr 93: 1481-1485. https://doi.org/10.1002/jsfa.5921

Bae S, Fleet GH, Heard GM, 2006. Lactic acid bacteria associated with wine grapes from several Australian vineyards. J Appl Microbiol 100: 712-727. https://doi.org/10.1111/j.1365-2672.2006.02890.x

Bell SJ, Henschke P, 2005. Implications of nitrogen nutrition for grapes, fermentation and wine. Austr J Grap Wine Res 11: 242-295. https://doi.org/10.1111/j.1755-0238.2005.tb00028.x

Benavent-Gil Y, Berbegal C, Lucio O, Pardo I, Ferrer S, 2016. A new fear in wine: Isolation of Staphylococcus epidermidis histamine producer. Food Cont 62: 142-149. https://doi.org/10.1016/j.foodcont.2015.10.026

Cao H, An J, Ou R, Lu L, Ai X, Yang Y, 2017. Enterobacter aerogenes: An emerging pathogen for enteritis in farmed channel catfish Ictalurus punctatus. Isr J Aquacult- Bamidgeh, 69. https://evols.library.manoa.hawaii.edu/handle/10524/56844

Cocolin L, 2000. Direct profiling of the yeast dynamics in wine fermentations. FEMS Microbiol Lett 189: 81-87. https://doi.org/10.1016/S0378-1097(00)00257-3

Coton E, Coton M, 2003. Microbiological origin of "Framboisé" in French ciders. J Inst Brew 109: 299-304. https://doi.org/10.1002/j.2050-0416.2003.tb00601.x

De Man JC, Rogosa M, Sharpe ME, 1960. A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23: 130-135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x

Death R, 2008. Encyclopedia of Ecology. Elsevier, Amsterdam, The Netherlands. 3122 pp.

Farouk S, Bela BEA, EL-Sharkawy HHA, 2017. The role of some elicitors on the management of Roumy Ahmar grapevines downy mildew disease and it's related to inducing growth and yield characters. Sci Hortic 225: 646-658. https://doi.org/10.1016/j.scienta.2017.07.054

Fedor PJ, Spellerberg IF, 2013. Reference module in earth systems and environmental sciences. https://www.elsevier.com/solutions/sciencedirect/content/reference-modules/earth-system

Flores-Rentería D, Rincón A, Valladares F, Curiel Yuste J, 2016. Agricultural matrix affects differently the alpha and beta structural and functional diversity of soil microbial communities in a fragmented Mediterranean holm oak forest. Soil Biol Biochem 92: 79-90. https://doi.org/10.1016/j.soilbio.2015.09.015

Galloway JN, Aber JD, Erisman, JW, Seitzinger SP, Howarth RW, Cowling EB, Cosby BJ, 2003. The nitrogen cascade. BioSci 53: 341-356. https://doi.org/10.1641/0006-3568(2003)053[0341:TNC]2.0.CO;2

Garde-Cerdán T, Portu J, López R, Santamaría P, 2016. Effect of methyl jasmonate application to grapevine leaves on grape amino acid content. Food Chem 203: 536-539. https://doi.org/10.1016/j.foodchem.2016.02.049

Garijo P, López R, Santamaría P, Ocón E, Olarte C, Sanz S, Gutiérrez AR, 2011. Presence of enological microorganisms in the grapes and the air of a vineyard during the ripening period. Eur Food Res Technol 233: 359-365. https://doi.org/10.1007/s00217-011-1528-3

González-Arenzana L, López R, Santamaría P, López-Alfaro I, 2012. Application of the different electrophoresis techniques to the detection and identification of lactic acid bacteria in wines. In: Electrophoresis. Intech, Croatia, pp: 137-156. https://doi.org/10.5772/45759

González-Arenzana L, Garijo P, Berlanas C, López-Alfaro I, López R, Santamaría P, Gutiérrez AR, 2016. Genetic and phenotypic intraspecific variability of non- Saccharomyces yeasts populations from La Rioja winegrowing region (Spain). J Appl Microbiol 133: 378-388. https://doi.org/10.1111/jam.13341

González-Arenzana L, Portu J, López R, Garijo P, Garde-Cerdán T, López-Alfaro I, 2017. Phenylalanine and urea foliar application: Effect on grape and must microbiota. Int J Food Microbiol 245: 88-97. https://doi.org/10.1016/j.ijfoodmicro.2017.01.017

Gramaje D, Mostert L, Groenewald JZ, Crous PW, 2015. Phaeoacremonium: From esca disease to phaeohyphomycosis. Fungal Biol 119: 759-783. https://doi.org/10.1016/j.funbio.2015.06.004

Harper DAT, 1999. Numerical Palobiology. John Willey and son. Toronto USA 468 pp.

Haytova D, 2013. Review of foliar fertilization of some vegetables crops. Ann Re Res Biol 3: 455-465.

He Q, Xu X, Zhang F, Tai Y, Luo Y, He J, Yan X, 2017. Production of chlorothalonil hydrolytic dehalogenase from agro-industrial wastewater and its application in raw food cleaning. J Sci Food Agric 97: 2582-2587. https://doi.org/10.1002/jsfa.8079

Hutzler M, Riedl R, Koob J, Jacob F, 2012. Fermentation and spoilage yeasts and their relevance for the beverage industry - A review. Brew Sci 65: 33-52.

Karydis M, Tsirtsis G, 1996. Ecological indices: a biometric approach for assessing eutrophication levels in the marine environment. Sci Total Environ 186: 209-219. https://doi.org/10.1016/0048-9697(96)05114-5

López I, Ruiz-Larrea F, Cocolin L, Orr E, Phister T, Marshall M, Mills DA, 2003. Design and evaluation of PCR primers for analysis of bacterial populations in wine by denaturing gradient gel electrophoresis. Appl Environ Microbiol 69: 6801-6807. https://doi.org/10.1128/AEM.69.11.6801-6807.2003

Margalef R, 1958. Information theory in ecology. Gen Syst 3: 36-71.

Mylona AE, Del Fresno JM, Palomero F, Loira I, Bañuelos MA, Morata A, Suárez-Lepe JA, 2016. Use of Schizosaccharomyces strains for wine fermentation - Effect on the wine composition and food safety. Int J Food Microbiol 232: 63-72. https://doi.org/10.1016/j.ijfoodmicro.2016.05.023

Osdaghi E, Taghavi SM, Fazliarab A, Elahifard E, Lamichhane JR, 2015. Characterization, geographic distribution and host range of Curtobacterium flaccumfaciens: An emerging bacterial pathogen in Iran. Crop Protect 78: 185-192. https://doi.org/10.1016/j.cropro.2015.09.015

Pérez-Martín F, Seseña S, Izquierdo PM, Palop ML, 2014. Are Enterococcus populations present during malolactic fermentation of red wine safe? Food Microbiol 42: 95-101. https://doi.org/10.1016/j.fm.2014.03.001

Portillo MC, Franquès J, Araque I, Reguant C, Bordons A, 2016. Bacterial diversity of Grenache and Carignan grape surface from different vineyards at Priorat wine region (Catalonia, Spain). Int J Food Microbiol 219: 56-63. https://doi.org/10.1016/j.ijfoodmicro.2015.12.002

Portu J, González-Arenzana L, Hermosín-Gutiérrez I, Santamaría P, Garde-Cerdán T, 2015a. Phenylalanine and urea foliar applications to grapevine: Effect on wine phenolic content. Food Chem 180: 55-63. https://doi.org/10.1016/j.foodchem.2015.02.008

Portu J, López-Alfaro I, Gómez-Alonso S, López R, Garde-Cerdán T, 2015b. Changes on grape phenolic composition induced by grapevine foliar applications of phenylalanine and urea. Food Chem 180: 171-180. https://doi.org/10.1016/j.foodchem.2015.02.042

Portu J, Santamaría P, López-Alfaro I, López R, Garde-Cerdán T, 2015c. Methyl jasmonate foliar application to Tempranillo vineyard improved grape and wine phenolic content. J Agric Food Chem 63: 2328-37. https://doi.org/10.1021/jf5060672

Portu J, López R, Baroja E, Santamaría P, Garde-Cerdán T, 2016. Improvement of grape and wine phenolic content by foliar application to grapevine of three different elicitors: Methyl jasmonate, chitosan, and yeast extract. Food Chem 201: 213-221. https://doi.org/10.1016/j.foodchem.2016.01.086

Ribéreau-Gayon P, Dubourdieu D, Donèche B, Lonvaud-Funel A, 2007. Handbook of Enology, The Microbiology of Wine and Vinifications (John Wille). Sussex. https://doi.org/10.1002/0470010398

Rinaudo M, 2006. Chitin and chitosan: Properties and applications. Progr Polym Sci 31: 603-632. https://doi.org/10.1016/j.progpolymsci.2006.06.001

Rodrigues N, Gonçalves G, Pereira-da-Silva S, Malfeito-Ferreira M, Loureiro V, 2001. Development and use of a new medium to detect yeasts of the genera Dekkera/Brettanomyces. J Appl Microbiol 90: 588-599. https://doi.org/10.1046/j.1365-2672.2001.01275.x

Ryan PD, Harper DAT, Whalley JS, 1995. Statistics for palaeontologists. Chapman & Hall.

Santamaría P, Garijo P, López R, Tenorio C, Gutiérrez AR, 2005. Analysis of yeast population during spontaneous alcoholic fermentation: Effect of the age of the cellar and the practice of inoculation. Int J Food Microbiol 103: 49-56. https://doi.org/10.1016/j.ijfoodmicro.2004.11.024

Suzuki H, Reddy MSS, Naoumkina M, Aziz N, May GD, Huhman DV, Dixon RA, 2004. Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula. Planta 220: 696-707. https://doi.org/10.1007/s00425-004-1387-2

Taraszkiewicz A, Fila G, Grinholc M, Nakonieczna J, 2013. Innovative strategies to overcome biofilm resistance. BioMed Res Int 2013: 150653. https://doi.org/10.1155/2013/150653

Villangó S, Pásti G, Kállay M, Leskó A, Balga I, Donkó A, Zsófi Z, 2015. Enhancing phenolic maturity of Syrah with the application of a new foliar spray. South Afr J Enol Vitic 36: 304-315. https://doi.org/10.21548/36-3-964

Walker GM, 2009. Encyclopedia of Microbiology. Elsevier, 3848 pp.




DOI: 10.5424/sjar/2019172-13471