Winter biodisinfestation with Brassica green manure is a promising management strategy for Phytophthora capsici control of protected pepper crops in humid temperate climate regions of northern Spain

  • Maite Gandariasbeitia NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
  • Mikel Ojinaga NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
  • Estibaliz Orbegozo NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
  • Amaia Ortíz-Barredo NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
  • Mireia Núñez-Zofío NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
  • Sorkunde Mendarte NEIKER, Conservation of Natural Resources Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
  • Santiago Larregla NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
Keywords: oospores, Capsicum annuum, biosolarization, biofumigation, Sinapis alba

Abstract

Phytophthora capsici causes root and crown rot of protected pepper (Capsicum annuum L.) crops in the Basque Country (northern Spain), a humid temperate climate area. The objective was to determine the effect of winter biofumigation and plastic cover (biodisinfestation) with Sinapis alba L. (cultivar 'Ludique') fresh green manure on the survival of introduced P. capsici inoculum (oospores) in a greenhouse soil. After autumn-winter Brassica cover crop soil incorporation in February, oospores remained at 15 and 30 cm depth for four weeks´ time exposure in two consecutive years, 2009 and 2010. Oospores viability was estimated with a plasmolysis method and infectivity with a potted pepper bioassay. Viability was significantly higher in biodisinfestated oospores than in the non-treated control in both years (81% and 21% relative increase in 2009 and 2010, respectively) and significantly higher at 30 cm depth than at 15 cm in 2009 (24% relative increase). Conversely, biodisinfestation significantly delayed disease incidence progression until the first half of infectivity bioassays in both years compared to the non-treated soils (83% and 75% relative decrease of dead plants in 2009 and 2010 respectively). The low soil temperatures could explain the low oospore survival reduction and suggest that the suppressiveness expressed in the infectivity bioassay was related with an increase of microbial activity in the biodisinfestation treatment. We conclude that winter biodisinfestation with fresh Brassica green manure is a promising management strategy for Phytophthora root rot control of protected peppers crops in regions of humid temperate climate such us northern Spain.

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Author Biography

Santiago Larregla, NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. C/ Berreaga 1, 48160 Derio (Bizkaia)
DPTO. PRODUCCION Y PROTECCION VEGETAL

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Published
2019-04-15
How to Cite
Gandariasbeitia, M., Ojinaga, M., Orbegozo, E., Ortíz-Barredo, A., Núñez-Zofío, M., Mendarte, S., & Larregla, S. (2019). Winter biodisinfestation with Brassica green manure is a promising management strategy for Phytophthora capsici control of protected pepper crops in humid temperate climate regions of northern Spain. Spanish Journal of Agricultural Research, 17(1), e1005. https://doi.org/10.5424/sjar/2019171-13808
Section
Plant protection