Reproductive performance of captive Senegalese sole, Solea senegalensis, according to the origin (wild or cultured) and gender

Ignacio Martín, Ignacio Carazo, Inmaculada Rasines, Cristina Rodríguez, Raquel Fernández, Paulino Martínez, Fernando Norambuena, Olvido Chereguini, Neil Duncan


Aim of study: The reproductive performance over a five year period in three different sole broodstocks: wild males with cultured females (WM), cultured males with wild females (CM), and a control of cultured males and females (C).

Area of study: Cantabria, Northern Spain.

Material and methods: The parental contribution was evaluated through the allocation of hatched larvae and a behavioural study was performed using underwater video recordings.

Main results: Fertilized natural spawns were only obtained from group WM showing a significantly higher mean annual volume of floating eggs compared with groups CM and C. Over the entire 5-yr a higher number of spawns, total and floating volumes of eggs were obtained compared with groups CM and C. The analysis of four polymorphic microsatellites enabled the identification of the individuals involved in the fertile spawns by parental assignment. The percentage of individuals contributing to spawns ranged between 56.3% and 75% showing fidelity patterns. However between 46.2% and 68.6% of the contribution to production was a result of the crossing of three single couples. According to the behaviour analysis, group WM presented the highest peak of activity at 20:00 h and a higher activity profile during the night, moreover, the courtship behaviours “swim follow” and “swim followed”, were only recorded in group WM.

Research highlights: The study has importance for aquaculture as it demonstrated how broodstocks with wild males and cultured females had adequate egg production for a commercial operation to supply eggs and implement single sex breeding programs.


reproduction; natural spawn; behaviour; parental contribution

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Agulleiro MJ, Anguis V, Cañavate JP, Martinez-Rodriguez G, Mylonas CC, Cerda J, 2006. Induction of spawning of captive-reared Senegal sole (Solea senegalensis) using different administration methods for gonadotropin-releasing hormone agonist. Aquaculture257: 511-524.

Anguís V, Cañavate JP, 2005. Spawning of captive Senegal sole (Solea senegalensis) under a naturally fluctuating temperature regime. Aquaculture 243: 133-145.

APROMAR, 2018. La acuicultura en España 2018. Asociación Empresarial de Productores de Cultivos Marinos, Chiclana (Cádiz), Spain.

Aristizabal E, Suarez J, Vega A, Bargas R, 2009. Egg and larval quality assessment in the Argentinean red porgy (Pagrus pagrus). Aquaculture 287: 329-334.

Bayarri MJ, Muñoz-Cueto JA, López-Olmeda JF, Vera LM, Rol de Lama MA, Madrid JA, Sánchez-Vázquez FJ, 2004. Daily locomotor activity and melatonin rhythms in Senegal Sole (Solea senegalensis). Physiol Behav 81: 577-583.

Brooks S, Tyler CR, Sumpter JP, 1997. Egg quality in fish: what makes a good egg? Rev Fish Biol Fish 7: 387-416.

Brown RC, Woolliams JA, McAndrew BJ, 2005. Factors influencing effective population size in commercial populations of gilthead seabream, Sparus aurata. Aquaculture 247: 219-225.

Carazo I, 2013. Reproductive behaviour and physiology of Senegalese sole (Solea senegalensis) broodstock in captivity. Thesis. University of Barcelona, Spain, 209 pp.

Carazo I, Norambuena F, Oliveira C, Sanchez-Vazquez FJ, Duncan NJ, 2013. The effect of night illumination, red and infrared light, on locomotor activity, behaviour and melatonin of Senegalese sole (Solea senegalensis) broodstock. Physiol Behav 118: 201-207.

Carazo I, Chereguini O, Martín I, Huntingford F, Duncan N, 2016. Reproductive ethogram and mate selection in captive wild Senegalese sole (Solea senegalensis). Span J Agric Res 14 (4): e0401.

Castanheira MF, Conceicao LEC, Millot S, Rey S, Begout ML, Damsgard B, Kristiansen T, Hoglund E, Overli O, 2017. Coping styles in farmed fish: consequences for aquaculture. Rev Aquacult 9: 23-41.

Castro J, Pino A, Hermida M, Bouza C, Riaza A, Ferreiro I, Sánchez L, Martínez P, 2006. A microsatellite marker tool for parentage analysis in Senegal sole (Solea senegalensis): Genotyping errors, null alleles and conformance to theoretical assumptions. Aquaculture 261: 1194-1203.

Dinis M, Ribeiro L, Soares F, Sarasquete C, 1999. A review on the cultivation potential of Solea senegalensis in Spain and in Portugal. Aquaculture 176: 27-38.

Fatsini E, Carazo I, Chauvigné F, Manchado M, Cerdà J, Hubbard PC, Duncan NJ, 2017a. Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids. J Exp Biol 220: 2057-2065.

Fatsini E, Rey S, Ibarra-Zatarain Z, Mackenzie S, Duncan NJ, 2017b. Dominance behaviour in a non-aggressive flatfish, Senegalese sole (Solea senegalensis) and brain mRNA abundance of selected transcripts. PLoS ONE 12 (9): e0184283.

Guzmán JM, Norberg B, Ramos J, Mylonas CC, Mañanos EL, 2008. Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis). Gen Comp Endocrinol 156: 285-297.

Guzmán JM, Ramos J, Mylonas CC, Mañanos EL, 2009. Spawning performance and plasma levels of GnRHa and sex steroids in cultured female Senegalese sole (Solea senegalensis) treated with different GnRHa-delivery systems. Aquaculture 291: 200-209.

Hardy OJ, Vekemans X, 2002. SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2: 618-620.

Herlin M, Delghandi M, Wesmajervi M, Taggart JB, McAndrew BJ, Penman DJ, 2008. Analysis of the parental contribution to a group of fry from a single day of spawning from a commercial Atlantic cod (Gadus morhua) breeding tank. Aquaculture 274: 218-224.

Howell B, Conceiçao L, Prickett R, Cañavate P, Mañanos E, 2009. Sole farming: nearly there but not quite?! Aquacult Eur 34: 24-27.

Ibarra-Zatarain Z, Fatsini E, Rey S, Chereguini O, Martín I, Rasines I, Alcaraz C, Duncan N, 2016. Characterization of stress coping style in Senegalese sole (Solea senegalensis) juveniles and breeders for aquaculture. R Soc Open Sci 3 (11): 160495.

Jia Y, Meng Z, Liu X, Lei J, 2014. Biochemical composition and quality of turbot (Scophthalmus maximus) eggs throughout the reproductive season. Fish Physiol Biochem 40: 1093-1104.

Kalinowski ST, Taper ML, Marshall TC, 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16: 1099-1106.

Mañanos E, Ferreiro I, Bolón D, Guzmán JM, Mylonas CC, Riaza A, 2007. Different responses of Senegalese sole Solea senegalensis broodstock to a hormonal spawning induction therapy, depending on their wild or captive-reared origin. Proc Aquacult Eur 07, Istanbul, Turkey, Oct 2007. Eur Aquacult Soc, 330-331.

Martín I, Rasines I, Gómez M, Rodriguez C, Martinez P, Chereguini O, 2014. Evolution of egg production and parental contribution in Senegalese sole, Solea senegalensis, during four consecutive spawning seasons. Aquaculture 424-425: 45-52.

Porta J, Porta JM, Martínez-Rodriguez G, Alvarez MC, 2006a. Development of a microsatellite multiplex PCR for Senegalese sole (Solea senegalensis) and its application to broodstock management. Aquaculture 256: 159-166.

Porta J, Porta JM, Martínez-Rodríguez G, Alvarez MC, 2006b. Genetic structure and genetic relatedness of a hatchery stock of Senegal sole (Solea senegalensis) inferred by microsatellites. Aquaculture 251: 46-55.

Taggart JB, 2006. FAP: an exclusion-based parental assignment program with enhanced predictive functions. Mol Ecol 7: 412-415.

Teng SK, El-Zahr C, Al-Abdul-Elah K, Almatar S, 1999. Pilot-scale spawning and fry production of blue-fin porgy, Sparidentex hasta (Valenciennes), in Kuwait. Aquaculture 178: 27-41.

Walsh PS, Metzger DA, Higuchi R, 1991. Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10: 506-513.

Wang J, 2002. An estimator for pairwise relatedness using molecular markers. Genetics 160: 1203-1215.

DOI: 10.5424/sjar/2019174-14953