Foliage applications of jasmonic acid modulate the antioxidant defense under water deficit growth in sugar beet

Hamideh Ghaffari, Mahmoud R. Tadayon, Muhammad Nadeem, Jamshid Razmjoo, Mumtaz Cheema


Aims of study: Water deficit (WD) is becoming an alarming problem in many regions of the world. Jasmonic acid (JA) is considered an important intracellular plant growth regulator. The aim of the current research was to investigate the important role of JA in mitigating the negative effects of WD on plant growth.

Area of study: Sugar beet production systems of two locations in Chaharmahal-Bakhtiari province, Iran.

Material and methods: A field trial was conducted to assess the foliar applications of JA (0, 5 µM and 10 µM) and WD (50%, 75%, 100% plant water requirements) effects on physiological yield components of sugar beet (Beta vulgaris L.) plants.

Main results: WD significantly (p<0.05) increased catalase, ascorbate and peroxidase activities, and malondialdehyde, hydrogen peroxide and white sugar content (WSC); however, it caused a reduction in white sugar yield and root yield (RY). JA foliage applications further enhanced the enzymes activity in WD treated plants resulting in higher WSC, potassium concentrations, white sugar and final RY. Interestingly the effects of JA applications were more pronounced under severe WD (50%) compared to mild (75%) or well-watered plants (100%). JA (10 µM) foliage applications increased the RY and white sugar production by 21% and 24% under severe WD.

Research highlights: JA can ameliorate the adverse effects of WD and increase the WD tolerance of sugar beet crop by upregulating the antioxidant enzyme activities to withstand adverse environmental conditions.


Beta vulgaris; antioxidant enzymes; irrigation requirement; lipid peroxidation; root yield; white sugar yield

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Abdel-Motagally FMF, Attia KK, 2009. Response of sugar beet plants to nitrogen and potassium fertilization in sandy calcareous soil. Int J Agric Biol 11: 695-700.

Aebi H, 1984. Catalase in vitro. Methods Enzymol 105: 121-126.

Ahmad P, Rasool S, Gul A, Sheikh SA, Akram NA, Ashraf M, Kazi AM, Gucel S, 2016. Jasmonates: multifunctional roles in stress tolerance. Front Plant Sci 7: 1-15.

Alam MM, Nahar K, Hasanuzzaman M, Fujita M, 2014. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnol Rep 8: 279-293.

Allen RG, Pereira LS, Raes D, Smith M, 2000. Crop evapotranspiration (guidelines for computing crop water requirements). FAO Irrig Drain Paper 56: 1-326.

Anjum SA, Xie X, Farooq M, Wang L, Xue L, Shahbaz M, Salhab J, 2011. Effect of exogenous methyl jasmonate on growth, gas exchange and chlorophyll contents of soybean subjected to drought. Afr J Biotechnol 10: 9640-9646.

Birben E, Sahiner UM, Sackesen C, Erzurum S, 2012. Oxidative stress and antioxidant defense. World Allergy Organ J 5 (1): 9-19.

Chołuj D, Karwowska R, Ciszewska A, Jasinska M, 2008. Influence of long-term droughtstress on osmolyte accumulation in sugar beet (Beta vulgaris L.) plants. Acta Physiol Plant 30: 679-687.

Chołuj D, Wiśniewskaa A, Szafrański KM, Cebulac J, Gozdowskid D, Podlaskia S, 2014. Assessment of the physiological responses to drought in different sugar beet genotypes in connection with their genetic distance. J Plant Physiol 171: 1221-1230.

Cunhua S, Wei D, Xiangling C, Xinna X, Yahong Z, Dong S, Jianjie S, 2010. The effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves. Afr J Biotechnol 9: 825-833.

De Ollas C, Hernando B, Arbona V, Gomez-Cadenas A, 2013. Jasmonic acid transient accumulation is needed for abscisic acid increase in citrus roots under drought stress conditions. Physiol Plant 147: 296-306.

De Vos CHR, Schat H, De Waal Vooijs, R, Ernst W, 1991. Increased to copper-induced damage of the root plasma membrane in copper tolerant Silene cucubalus. Plant Physiol 82: 523-528.

Din J, Khan SU, Ali I, Gurmani AR, 2011. Physiological and agronomic response of canola varieties to drought stress. J Anim Plant Sci 21 (1): 78-82.

Fotouhi K, Heravan ES, Rajabi A, Azizinejad R, 2017. Screening sugar beet genotypes in drought stress condition using tolerance indices. Agri Forest 63 (2): 105-109.

Gao XP, Wang XF, Lu YF, Zhang LY, Shen YY, Liang Z, Zhang DP, 2004. Jasmonic acid is involved in the water-stressed-induced betaine accumulation in pear leaves. Plant Cell Environ 27: 497-507.

Ghaffari H, Tadayon MR, Nadeem M, Cheema M, Razmjoo J, 2019. Proline mediated changes in antioxidant enzymatic activities and physiology of sugar beet under drought stress. Acta Physiol Plant 41 (2): 23.

Ghamarina H, Arji I, Sepehri S, Norozpour S, Khodael E, 2012. Evaluation and comparison of drip and convential irrigation methods on sugar beets in a semi-arid regions. J Irrig Drain Eng 138: 90-97.

Gholami Zali A, Ehsanzadeh P, 2018. Exogenous proline improves osmoregulation, physiological functions, essential oil, and seed yield of fennel. Ind Crops Prod 111: 133-140.

Hasanuzzaman M, Fujita M, 2011. Selenium pretreatment up-regulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings. Biol Trace Elem Res 143: 1758-1776.

Herzog V, Fahimi H, 1973. Determination of the activity of peroxidase. Anal Biochem 55: 554-562.

Hoffmann CM, 2010. Sucrose accumulation in sugar beet under drought stress. J Agric Crop Res 196: 243-252.

Iqbal MA, Saleem AM, 2015. Sugar beet potential to beat sugarcane as a sugar crop in Pakistan. J Agr Environ Sci 15 (1): 36-44.

Kadkhodaie A, Zahedi M, Razmjoo J, Pessarakli M, 2014. Changes in some anti-oxidative enzymes and physiological indices among sesame genotypes (Sesamum indicum L.) in response to soil water deficits under field conditions. Acta Physiol Plant 36: 641-650.

Leufen G, Noga G, Hunsche M, 2016. Drought stress memory in sugar beet: mismatch between biochemical and physiological parameters. J Plant Growth Regul 35: 680-689.

Lin CC, Kao CH, 2000. Effect of NaCl stress on H2O2 metabolism in rice leaves. J Plant Growth Regul 30 (2): 151-155.

Liu W, Sun F, Lim WH, Zhang J, Wang H, Shiogama H, Zhang Y, 2018. Global drought severe drought-affected populations in 1.5 and 2oC warmer worlds. Earth Syst Dynam 9: 267-283.

Mahmoud EA, Hassanin MA, Borham TI, Emara EIR, 2018. Tolerance of some sugar beet varieties to water stress. Agric Water Manag 201: 144-151.

Mahmood M, Bidabadi SS, Ghobadi C, Gray DJ, 2012. Effect of methyl jasmonate treatments on alleviation of polyethylene glycol-mediated water stress in banana (Musa acuminata cv. 'Berangan', AAA) shoot tip cultures. J Plant Growth Regul 68: 161-169.

Majid NA, Akbar N, 2006. A possible role for methyl jasmonate in effecting superoxide dismutase and catalase activities under PQ-induced oxidative stress in maize seedlings. J Biol Sci 6: 55-60.

Mancosu N, Snyder RL, Kyriakakis G, Spano D, 2015. Water scarcity and future challenges for food production. Water 7: 975-992.

Mansuri M, Babazadeh H, Emdad MR, Taleghani D, 2018. Effect of deficit irrigation management on qualitative and quantitative yield of sugar beet (Beta vulgaris L.) in Karaj, Iran. Appl Ecol Env Res 16 (1): 455-466.

Märländer B, Hoffmann C, Koch HJ, Ladewig E, Merkes R, Petersen J, Stockfisch N, 2003. Environmental situation and yield performance of the sugar beet crop in Germany: heading for sustainable development. J Agron Crop Sci 189: 201-226.

Masri MI, Ramadan BS, El-Shafai AM, El-Kady MS, 2015. Effect of water stress and fertilization on yield and quality of sugar beet under drip and sprinkler irrigation systems in sandy soil. Int J Agr Sci 5 (3): 414-425.

Mishra AK, Singh VP, 2010. A review of drought concepts. J Hydrol 391: 202-216.

Moosavi SGR, Ramazani SHR, Sadeghzadeh Hemayati S, Gholizade H, 2017. Effect of drought stress on root yield and some morpho-physiological traits in different genotypes of sugar beet (Beta vulgaris L.). J Crop Sci Biotechnol 20 (3): 167-174.

Moussa H, Abdel-Aziz SM, 2008. Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Aust J Crop Sci 1: 31-36.

Muñoz-Espinoza VA, López-Climent MF, Casaretto JA, Gómez-Cadenas A, 2015. Water stress responses of tomato mutants impaired in hormone biosynthesis reveal abscisic acid, jasmonic acid and salicylic acid interactions. Front Plant Sci 6: 1-14.

Nahar S, Kalita J, Sahoo L, Tanti B, 2016. Morphophysiological and molecular effects of drought stress in rice. Ann Plant Sci 5: 1409-1416.

Nakano Y, Asada K, 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867-880.

Nazarli H, Ahmadi A, Hadian J, 2014. Salicylic acid and methyl jasmonate enhance drought tolerance in chamomile plants. J Herb Pharm 3 (2): 87-92.

Neseim MR, Amin AY, El-Mohammady MMS, 2014. Effect of potassium applied with foliar spray of yeast on sugar beet growth and yield under drought stress. Glo Res J Agric Sci 3 (8): 211-222.

Norastehnia A, Nojavan-Asghari M, 2006. Effect of methyl jasmonate on the enzymatic antioxidant defense system in maize seedling subjected to paraquat. As J Plant Sci 5: 17-23.

Ober E, 2001. The search for drought tolerance in sugar beet. Brit Sugar Beet Rev 69 (1): 40-43.

Pazoki AR, 2015. Influence of salicylic and jasmonic acid on chlorophylls, carotenes and xanthophylls contents of lemon balm (Melissa officinalis L.) under salt stress conditions. Biol Forum 7 (1): 287-292.

Poonam S, Kaur H, Geetika S, 2013. Effect of jasmonic acid on photosynthetic pigments and stress markers in Cajanus cajan (L.) Millsp. seedlings under copper stress. Am J Plant Sci 4: 817-823.

Rakwal R, Komatsu S, 2005. Abscisic acid promoted changes in the protein profiles of rice seedling by proteome analysis. Mol Biol Rep 31: 217-230.

Reinfeld E, Emmerich, G Baumgarten C, Winner, Beiss U, 1974. Zur Voraussage des Melassez zuckersaus Ruben analysen Zucker. In: The sugar beet crop; Cooke DA, Scott RK (eds). pp: 2-15. First end, Chapman & Hall, World Crop Series, London, UK.

Shan CJ, Liang ZS, 2010. Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Plant Sci 178: 130-139.

Sharma P, Jha AB, Dubey RS, Pessarakli M, 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 2012: Art ID 217037.

Topak R, Süheri S, Acar B, 2011. Effect of different drip irrigation regimes on sugar beet (Beta vulgaris L.) yield, quality and water use efficiency in Middle Anatolian, Turkey. Irrig Sci 29: 79-89.

Yu CW, Murphy TM, Lin CH, 2003. Hydrogen peroxide induces chilling tolerance in mung beans mediated through ABA-independent glutathione accumulation. Funct Plant Biol 30: 955-963.

DOI: 10.5424/sjar/2019174-15380