Estimation of vineyard leaf area by linear regression

P. Sanchez-de-Miguel, P. Junquera, M. de la Fuente, L. Jimenez, R. Linares, P. Baeza, J. R. Lissarrague


Vineyard leaf area is a variable that must be determined when assessing the productive potential of a vineyard and for characterizing the light and thermal microenvironments of grapevine plants. The aim of the present work was to validate the Lopes and Pinto method for determining vineyard leaf area in the vineyards of central Spain and with the area’s cultivars. The results obtained were compared to those provided by a traditional and accurate —but much more laborious— non-destructive direct method. Experiments were performed over three years in six vineyards growing either cvs. Syrah, Cabernet Sauvignon, Cabernet franc, Merlot or Tempranillo. Good agreement was found between the two methods both in the determination of primary and lateral shoot leaf areas for all cultivars and vineyards. The simplicity of the Lopes and Pinto method means much larger sample sizes can be examined in the same period of time, increasing the accuracy of final vineyard leaf area values. In fact, regression analysis of the data collected for the Lopes and Pinto method showed that only three field-measured variables need to be recorded for the inspected shoots of either type: the area of the largest leaf, the area of the smallest leaf, and the number of leaves.


Cabernet franc; Cabernet Sauvignon; destructive methods; Merlot; non-destructive methods; Syrah; Tempranillo; Vitis vinifera

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Barbagallo M.G., Lorenzo R.I., Motisi A., Sottile I., 1996. Estimation of leaf area changes along the primary shoot in grapevine. Proc 9th GESCO Meeting, Univ Horticulture and Food Industries, Budapest, Hungary. pp. 245-250.

Beslic Z., Todic S., Tesic D., 2010. Validation of non-destructive methodology of grapevine leaf area estimation on cv. Blaufränkisch (Vitis vinifera L.). S Afr J Enol Vitic 31(1), 22-25.

Candolfi-Vasconcelos M.C., Koblet W., 1990. Yield, fruit quality, bud fertility and starch reserves of the wood as a function of leaf removal in Vitis vinifera-evidence of compensation and stress recovering. Vitis 29, 199-221.

Carbonneau A., 1976. Principes et méthodes de mesure de la surface foliaire. Essai de caractérisation del types de feuilles dans le genre Vitis. Annales de l'Amelioration des Plantes 26(2), 327-343. [In French].

Carbonneau A., 1989. L'exposition utile du feuillage: definition du potentiel du système de conduite. In: Systeme de conduite de la vigne et mecanisation. OIV, Paris. pp. 13-33. [In French].

Champagnol F., 1984. Elements de physiologie de la vigne et de viticulture generale. Dehan, Montpellier. 351 pp. [In French].

Cloete H., Archer E., Hunter J.J., 2006. Shoot heterogeneity effects on Shiraz/Richter 99 grapevines. I. Vegetative growth. S Afr J Enol Vitic 27(1), 68-75.

Cloete H., Archer E., Novello V., Hunter J.J., 2008. Shoot heterogeneity effects on Shiraz/Richter 99 grapevines. II. Physiological activity. S Afr J Enol Vitic 29(1), 1-8.

Cohen S., Striem M.J., Bruner M., Klein I., 2000. Grapevine leaf area index evaluation by gap fraction inversion. Acta Hortic 537, 87-94.

Dobrowski S.Z., Ustin S.L., Wolpert J.A., 2002. Remote estimation of vine canopy density in vertically shoot-positioned vineyards: determining optimal vegetation indexes. Aust J Grape Wine Res 8, 117-125.

Hale C.R., Weaver R.J., 1962. The effect of developmental stage on direction of translocation of photosynthate in Vitis vinifera. Hilgardia 33, 89-131.

Howell S.G., 2001. Sustainable grape productivity and the growth-yield relationship: a review. Am J Enol Vitic 52(3), 165-174.

Huglin P., Schneider C., 1998. Biologie et écologie de la vigne. Lavoisier, TEC et DOC, Paris. 370 pp. [In French].

Intrieri C., Poni S., Silvestroni O., Filippetti I., 1992. Leaf age, leaf position and photosynthesis in potted grapevines. Adv Hort Sci 1, 23-27.

Jackson D.I., Lombard P.B., 1993. Environmental and management practices affecting grape composition and wine quality-a review. Am J Enol Vitic 44(4), 409-430.

Kleijnen J.P.C., 1987. Statistical tools for simulation modeling and analysis. McGraw-Hill, NY, USA. 429 pp.

Kliewer W.M., Dokoozlian N.K., 2000. Influence of canopy management on canopy microclimate and composition of grapes – a review. 6th Int Symp On Grapevine Phys and Biotech, Heraklion, Greece.

Kliewer W.M., Dokoozlian N.K., 2005. Leaf area/crop weight ratios of grapevines: influence on fruit composition and wine quality. Am J Enol Vitic 56(2), 170-181.

Lopes C.M., Pinto P., 2000. Estimation de la surface foliaire principale et secondaire d'un rameau de vigne. Progrès Agricole et Viticole 117(7), 160-166. [In French].

Lopes C., Pinto P.A., 2005. Easy and accurate estimation of grapevine leaf area with simple mathematical models. Vitis 44(2), 55-61.

Lorenz D.H., Eichhorn K.W., Bleiholder H., Klose R., Meier U., Webe E., 1995. Growth stages of the grapevine. Aust J Grape Wine Res 1, 100-110.

Oliveira M., Santos M., 1995. A semi-empirical method to estimate canopy leaf area of vineyards. Am J Enol Vitic 46(3), 389-391.

Ollat N., Fermaud M., Tandonnet J.P., Neveux M., 1998. Evaluation of an indirect method for leaf area index determination in the vineyard: combined effects of cultivar, year and training system. Vitis 37, 73-78.

Ollat N., Diakou-Verdin P., Carde J.P., Barrieu F., Gaudillère J.P., Moing A., 2002. Grape berry development: a review. J Int Sci Vigne Vin 36(3), 109-131.

Palliotti A., Cartechini A., Ferranti F., 2000. Morpho-anatomical and physiological characteristics of primary and lateral shoot leaves of Cabernet Franc and Trebbiano Toscano grapevines under two irradiance regimes. Am J Enol Vitic 51(2), 122-130.

Patakas A., Noitsakis B., 1999. An indirect method of estimating leaf area index in cordon trained spur pruned grapevines. Sci Horticult 80, 299-305.

Petrie P.R., Trought M.C.T., Howell G.S., 2000a. Fruit composition and ripening of Pinot Noir (Vitis vinifera L.) in relation to leaf area. Aust J Grape Wine Res 6, 46-51.

Petrie P.R., Trought M.C.T., Howell G.S., 2000b. Growth and dry matter partitioning of Pinot Noir (Vitis vinifera L.) in relation to leaf area and crop load. Aust J Grape Wine Res 6, 40-45.

Reynolds A.G., Heuvel J.E.V., 2009. Influence of grapevine training systems on vine growth and fruit composition: a review. Am J Enol Vitic 60(3), 251-268.

Sánchez-De-Miguel P., Baeza P., Junquera P., Lissarrague J.R., 2010. Vegetative development: total leaf area and surface area indexes. In: Methodologies and results in grapevine research (Delrot S., Medrano H., Or E., Bavaresco L., Grando S., eds). Springer, Dordrecht. pp. 31-44.

Schultz H.R., 1992. An empirical model for the simulation of leaf appearance and leaf development of primary shoots of several grapevine (Vitis vinifera L.). Sci Horticult 52, 179-200.

Schultz H.R., 1995. Grape canopy structure, light microclimate and photosynthesis I. A two dimensional model of the spatial distribution of surface area densities and leaf ages in two canopy systems. Vitis 34(4), 211-215.

Smart R.E., 1985. Principles of grapevine canopy microclimate manipulation with implications for yield and quality. A review. Am J Enol Vitic 36(3), 230-239.

Smart R.E., Robinson M., 1991. Sunlight into wine: a handbook for winegrape canopy management. Winetitles, Adelaide, Australia. 88 pp.

Sommer K.J., Lang A.R.G., 1994. Comparative analysis of two indirect methods of measuring LAI as applied to minimal and spur pruned grape vines. Aust J Plant Physiol 21, 197-206.

Spann T.M., Heerema R.J., 2010. A simple method for non-destructive estimation of total shoot leaf area in tree fruit crops. Sci Horticult 125,528-533.

Wermelinger B., Kobletw., 1990. Seasonal growth and nitrogen distribution in grapevine leaves, shoots and grapes. Vitis 29, 15-26.

Williams L.E., 1987. Growth of «Thompson seedless» grapevines: I- Leaf area development and dry weight distribution. J Am Soc Hort Sci 112(2), 325-330.

Zufferey V., Murisier F., Monney P., 1998. Interception du rayonnement solaire par la vigne selon différntes dispositions de la haie foliaire. Revue Suisse Vitic Arboric Hortic 30(5), 301-304. [In French].

Zufferey V., Murisier F., Schultz R., 2000. A model analysis of the photosynthetic response of Vitis vinifera L. cvs. Riesling and Chasselas leaves in the field: I. Interaction of age, light and temperature. Vitis 39(1), 19-26.

DOI: 10.5424/sjar/20110901-354-10