Survival percentage, photosynthetic abilities and growth characters of two indica rice (Oryza sativa L. spp. indica) cultivars in response to iso-osmotic stress

T. Nishimura, S. Cha-um, M. Takagaki, K. Ohyama, C. Kirdmanee

Abstract


The aim of this study was to investigate pigment degradation, chlorophyll fluorescence diminution, photosynthetic ability and growth reduction in two rice cultivars, in response to either iso-osmotic salt stress or water-deficit stress. Seedlings of rice cultivars RD6 and KDML105 were photo-autotrophically grown in MS media and subsequently exposed to –0.23 (control), –0.40 or –0.67 MPa iso-osmotic NaCl (salt stress) or mannitol (water-deficit stress) for 14 days. The survival percentage of the two rice cultivars reduced dramatically when subjected to –0.67 MPa NaCl treatment. Chlorophyll a (Chla), chlorophyll b (Chlb), total carotenoids (Cx+c), maximum quantum yield of PSII (Fv/Fm) and photon yield of PSII (ΦPSII) in the stressed seedlings were significantly lower when compared to seedlings in the control group (without mannitol or NaCl), leading to low net-photosynthetic rate (Pn) and growth reduction. In addition, the growth characters of plantlets in the salt stress conditions were more sharply reduced, and the physiological changes greater than those in water-deficit stress conditions. On the other hand, non-photochemical quenching in the leaves of stressed plantlets increased significantly, especially in response to iso-osmotic salt stress. In the present study, overall growth performance and physiological characters of KDML105 grown under iso-osmotic stress were better than those of RD6.


Keywords


net-photosynthetic rate, photosynthetic pigments, salt stress, water deficit

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References


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DOI: 10.5424/sjar/20110901-162-10