Short communication: Development of a new polymorphic genetic marker in Araucaria araucana (Mol) K. Koch
Abstract
Seed storage proteins have been used as genetic marker in forest species to evaluate genetic variability, demonstrating its effectiveness both in conifers and broad-leaved. In conifers, megagametophyte storage proteins are particularly useful because of their haploid nature. The aim of this study was to determine whether these proteins could be used as a new marker of genetic diversity in Araucaria araucana, one of the oldest conifers of South America and a representative symbol of Chilean forest biodiversity. For this, megagametophytes from two A. araucana populations were assessed to identify polymorphic bands and to obtain a preliminary estimation of the genetic diversity. The results revealed that globulin is the best fraction for measuring the variability in the species, due to their high level of variation (20 identified bands, eleven of them polymorphic). Both populations showed high genetic diversity, with more than 92% of the variation within populations. The study highlighted that these proteins can be used to measure the genetic diversity in A. araucana, providing good information to ensure the preservation of the species genetic resources.
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References
Allona I, Collada C, Casado R, Aragoncillo C, 1994. Electrophoretic analysis of seed storage proteins from gymnosperms. Electrophoresis 15: 1062-1067. http://dx.doi.org/10.1002/elps.11501501158 PMid:7859708
Alvarez JB, Muñoz C, Martín MA, López S, Martín LM, 2003. Cotyledon storage proteins as markers of the genetic diversity in Castanea sativa Miller. Theor Appl Genet 107: 730-735. http://dx.doi.org/10.1007/s00122-003-1298-9 PMid:12750777
Alvarez JB, Toledo MJ, Abellanas B, Martín LM, 2004. Use of megagametophyte storage proteins as markers of the genetic diversity in stone pine (Pinus pinea L) in Andalusia, Spain. Genet Res Crop Evol 51: 621-627. http://dx.doi.org/10.1023/B:GRES.0000024647.74194.4e
Bekessy SA, Allnut TR, Premoli AC, Lara A, Ennos RA, Burgman MA, Cortes M, Newton AC, 2002. Genetic variation in the vulnerable and endemic monkey puzzle tree, detected using RAPDs. Heredity 88: 243-249. http://dx.doi.org/10.1038/sj.hdy.6800033 PMid:11920130
Bekessy SA, Ennos RA, Burgman MA, Newton AC, Ades PR, 2003. Neutral DNA markers fail to detect genetic divergence in an ecologically important trait. Biol Conserv 110: 267-275. http://dx.doi.org/10.1016/S0006-3207(02)00225-2
Burns BR, 1993. Fire-induced dynamics of Araucaria araucana-Nothofagus antartica forest in the southern Andes. J Biogeogr 20: 669-685. http://dx.doi.org/10.2307/2845522
Drake F, 2004. Uso sostenible en bosques de Araucaria araucana (Mol.) K. Koch. Aplicación de modelos de gestión. Doctoral thesis, Universidad de Córdoba, Spain. [In Spanish].
Drake F, Martín MA, Herrera MA, Molina JR, Drake-Martin F, Martín LM, 2009. Networking sampling of Araucaria araucana (Mol.) K. Koch in Chile and the bordering zone of Argentina: implications for the genetic resources and the sustainable management. iForest 2: 207-212.
El-Kassaby YA, Meagher MD, Parkinson J, Portlock FT, 1987. Allozyme inheritance, heterozygosity and outcrossing rate among Pinus monticola near Ladysmith, British Columbia. Heredity 58: 173-181. http://dx.doi.org/10.1038/hdy.1987.31
Fonseca PA, Ferreira RB, Texeira AR 1997. Seed proteins from Quercus suber. J Agr Food Chem 45: 3443-3447. http://dx.doi.org/10.1021/jf9609486
Gallo L, 2003. Conservación, manejo y uso sustentable de los recursos genéticos de la Araucaria araucana en Argentina, Comunidades Aucapan y Chiuquilihuin, San Carlos de Bariloche. Report of the Project “Conservation, Management and Sustainable Use of Forest Genetic Resources with reference to Brazil and Argentina”. International Plant Genetic Resources Institute, Rome, Italy. [In Spanish].
Gepts P, 1990. Genetic diversity of seed storage proteins in plants. In: Plant population genetics, breeding and genetic resources (Brown AHD, Clegg MT, Kahler AL, Weir BS eds.). Sinauer Assoc Inc Publ, Suderland, MA, USA. pp: 64-82.
Hoffmann A, Sierra M, Prosser C, Valle M, 2001. Enciclopedia de los bosques chilenos. Gráfica Andes, Santiago de Chile. [In Spanish]. PMid:5432063
IUCN, 1996. World list of threatened trees. International Union for the Conservation of Nature, Gland, Switzerland. Available in: http://www.iucn.org/about/work/programmes/species/red_list.htm. [11 January 2012].
Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. http://dx.doi.org/10.1038/227680a0
Marchelli P, Baier C, Mengel C, Ziegenhagen B, Gallo LA, 2010. Biogeographic history of the threatened species Araucaria araucana (Molina) K. Koch and implications for conservation: a case study with organelle DNA markers. Cons Genet 11: 951-963. http://dx.doi.org/10.1007/s10592-009-9938-5
Martín MA, Navarro-Cerrillo R, Ortega P, Alvarez JB, 2009. The use of cotyledon proteins to assess the genetic diversity in sweet holm oak. J For Sc 55: 526-531.
Martín MA, Alvarez JB, Martín LM, 2010a. Genetic diversity of Spanish fir (Abies pinsapo Boiss.) populations by means of megagametophyte storage proteins. Ann For Sci 67: 603 (7p).
Martín MA, Muñoz S, Muñoz F, Uribe M, Molina JR, Herrera MA, Martín LM, Alvarez JB, 2010b. Primeros resultados en el desarrollo de un marcador genético basado en las proteínas de reserva en especies del género Nothofagus. Bosque 31, 252-257. [In Spanish].
Metakovsky EV, Gómez M, Vázquez F, Carrillo JM, 2000. High genetic diversity of Spanish common wheats as judged from gliadin alleles. Plant Breeding 119: 37-42. http://dx.doi.org/10.1046/j.1439-0523.2000.00450.x
Morris RW, Spieth PT, 1978. Sampling strategies for using female gametophytes to estimate heterozygosity in conifers. Theor Appl Genet 51: 217-222. http://dx.doi.org/10.1007/BF00273768
Neale D, Adams WT, 1981. Inheritance of isozyme variants in seed tissues of balsam fir (Abies balsamea). Can J Bot 59: 1285-1291. http://dx.doi.org/10.1139/b81-172
Nei M, 1973. Analysis of gene diversity in subdivided populations. P Natl Acad Sci USA 70: 3321-3323. http://dx.doi.org/10.1073/pnas.70.12.3321
Osborne TB, 1924. The vegetable proteins. Longmans, Green & Co. London.
Payne PI, Holt LM, Worland AJ, Law CN, 1982. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. 3. Telocentric mapping of the subunits genes on the long arms of the homoeologous group 1 chromosomes. Theor Appl Genet 63: 129-138. http://dx.doi.org/10.1007/BF00303695
Ruiz E, Gonzalez F, Torres-Diaz C, Fuentes G, Mardones M, Stuessy T, Rosabelle S, Becerra J, Silva M, 2007. Genetic diversity and differentiation within and among Chilean populations of Araucaria araucana (Araucariaceae) based on allozyme variability. Taxon 56: 1221-1228. http://dx.doi.org/10.2307/25065913
Veblen TT, 1982. Regeneration patterns in Araucaria araucana forest in Chile. J Biogeog 9: 11-28. http://dx.doi.org/10.2307/2844727
Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX, 1997. Popgene ver 1.32. The user-friendly software for population genetic analysis. Mol Biol Biotechnol Cent, Univ Alberta, Canada.
Zillman RR, Bushuk W, 1979. Wheat cultivar identification by gliadin electropherograms. III. Catalogue of electropherogram formulas of Canadian wheat cultivars. Can J Plant Sci 59: 287-298. http://dx.doi.org/10.4141/cjps79-048
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