Short communication. Zebrafish embryo development can be reversibly arrested at the MBT [mid blastula transition] stage by exposure to a water temperature of 16 deg C

M. Francisco Simão, M. Perez Camps, F. García-Ximénez

Abstract


Germline chimaerism (intra or interspecific) is a technique of great potential in aquaculture. It allows specimens to be obtained that produce gametes whose origins lie in the cells of the donor organism. Chimaerism is usually performed at the mid blastula transition (MBT) stage since this is the last in which embryonic cells remain completely totipotent. Zebrafish are photoperiodic in their egg-laying behaviour and show rapid embryonic development. For chimaerism to be successful, it is of interest to establish the maximum time over which embryonic development can be reversibly arrested. This paper reports the effect on survival of subjecting zebrafish embryos at different stages of development to a water temperature of 16 deg C for different lengths of time. The maximum exposure time after which these embryos were able to resume development following low-temperature-induced developmental arrest became shorter as the embryonic stage exposed became earlier. At the MBT stage, the maximum safe exposure time was 2 h; longer exposure times led to problems in development and survival.

Keywords


DANIO RERIO; HYBRIDIZATION; ANIMAL EMBRYOS; EMBRYO CULTURE; GAMETES; CHIMAERAS; EMBRYONIC DEVELOPMENT; WATER TEMPERATURE; COLD; FISH CULTURE; ANIMAL MODELS; EXPERIMENTATION

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DOI: 10.5424/sjar/2007052-244