Evaluation of repeated biodisinfestation using Brassica carinata pellets to control Meloidogyne incognita in protected pepper crops

  • M. M. Guerrero-Díaz Dept. de Biotecnología y Protección de Cultivos, IMIDA, Mayor s/n 30150 La Alberca (Murcia)
  • C. M. Lacasa-Martínez Dept. de Biotecnología y Protección de Cultivos, IMIDA, Mayor s/n 30150 La Alberca (Murcia)
  • A. Hernández-Piñera Dept. de Biotecnología y Protección de Cultivos, IMIDA, Mayor s/n 30150 La Alberca (Murcia)
  • V. Martínez-Alarcón Dept. de Biotecnología y Protección de Cultivos, IMIDA, Mayor s/n 30150 La Alberca (Murcia)
  • A. Lacasa-Plasencia Dept. de Biotecnología y Protección de Cultivos, IMIDA, Mayor s/n 30150 La Alberca (Murcia)
Keywords: soil, nematodes, organic amendments, greenhouses

Abstract

The nematode Meloidogyne incognita is responsible for substantial losses in greenhouse-grown peppers in south-eastern Spain. This study evaluates the use of biodisinfestation (BS) (organic amendment + solarisation) as an alternative to using methyl bromide (MB) over three consecutive years to control the nematode in greenhouse conditions. Brassica carinata (BP) pellets or B. carinata (BP) + fresh sheep manure (M) were evaluated in treatments which began on two different dates (August and October) and the results were compared with MB-disinfested and untreated controls. During the third year, the gall index for BP was lower than that obtained for BP+M and in the August treatment than in the October treatment. The commercial crop of pepper fruit obtained with the biodisinfestation treatments begun in August was similar to or higher than that obtained with MB, and higher than that obtained with both October biodisinfestation treatments. The yield of the October biodisinfestation treatments was higher than that of the untreated one. In August of all the years studied, the accumulated exposure times were greater than the thresholds required to kill M. incognita populations at 15 cm depth. The incidence of the nematode did not correspond to the reduction achieved during solarisation, and seemed to increase during the crop cycle. Further studies should look at why high temperatures do not produce a sustained reduction in the populations of Meloidogyne incognita.

Downloads

Download data is not yet available.

References

Arriaga H, Núñez-Zofío M, Larregla S, Merino P, 2011. Gaseous emissions from soil biodisinfestation by animal manure on a greenhouse pepper crop. Crop Prot 30: 412-419.
http://dx.doi.org/10.1016/j.cropro.2010.12.012 

Bello A, López-Pérez JA, García-Álvarez A, Arcos SC, Ros C, Guerrero MM, Guirao P, Lacasa A, 2004. Biofumigación con solarización para el control de nematodos en cultivo de pimiento. In: Desinfección de suelos en invernaderos de pimiento (Lacasa A, Guerrero MM, Oncina M, Mora JA, eds.). Publicaciones de la Consejería de Agricultura, Agua y Medio Ambiente, Región de Murcia (Spain), pp: 105-129.
PMid:15516831  

Blok WJ, Lamers JG, Termorshuizen AJ, Bollen GJ. 2000. Control of soilborne plant pathogens by incorporating fresh organic amendments followed by tarping. Phytopathology 90: 253-259.
http://dx.doi.org/10.1094/PHYTO.2000.90.3.253
PMid:18944617  

Bonanomi G, Antignani V, Pane C, Scala F, 2007. Suppression of soilborne fungal diseases with organic amendments. J Plant Pathol 89: 311-324. 

Bonanomi G, Antignani V, Capodilupo M, Scala F, 2010. Identifying the characteristics of organic soil amendments that suppress soil-borne plant diseases. Soil Biol Biochem 42: 136-144.
http://dx.doi.org/10.1016/j.soilbio.2009.10.012 

Bridge J, Page SLJ, 1980. Estimation of root-knot infestation levels in roots using a rating chart. Trop Pest Manage 26: 296-298.
http://dx.doi.org/10.1080/09670878009414416 

Candido V, Miccolis V, Basile M, D’Addabbo T, Gatta G, 2005. Soil solarization for the control of Meloidogyne javanica on eggplant in Southern Italy. Acta Hort 698: 195-199. 

CAARM, 2010. Normas técnicas de producción integrada en cultivo de pimiento. Consejería de Agricultura y Agua de la Región de Murcia. BORM 134: 32348-32364. 

Chellemi DO, Olson SM, Scott JW, Mitchell DJ, McSorley R, 1993. Reduction of phytoparasitic nematodes on tomato by solarization and genotype. J Nematol 25: 800-805.
PMid:19279843 PMCid:2619444 

Cohen MF, Yamasaki H, Mazzola M, 2005. Brassica napus seed meal soil amendment modifies microbial community structure, nitric oxide production and incidence of Rhizoctonia root rot. Soil Biol Biochem 37: 1215-1227.
http://dx.doi.org/10.1016/j.soilbio.2004.11.027 

Everts KL, Sardanelli S, Kratochvil RJ, Armentrout DK, Gallagher LE, 2006. Root-knot and root-lesion nematode suppression by cover crops, poultry litter, and poultry litter compost. Plant Dis 90: 487-492.
http://dx.doi.org/10.1094/PD-90-0487 

Gamliel A, Austerweil M, Kritzman G, 2000. Non-chemical approach to soilborne pest management - organic amendments. Crop Prot 19: 847-853.
http://dx.doi.org/10.1016/S0261-2194(00)00112-5 

Gamliel A, Gadkar V, Zilberg V, Beniches M, Rabinowich E, Manor H, Wininger S, Kapulnik Y, 2004. Effect of solarization intensity on the control of pink root of chives, and the response of the crop to AM fungal application. Symbiosis 37: 233-247. 

Guerrero MM, Lacasa A, Ros C, Bello A, Martínez MC, Torres J, Fernández P, 2004a. Efecto de la biofumigación con solarización sobre los hongos del suelo y la producción: fechas de desinfección y enmiendas. In: Desinfección de suelos en invernaderos de pimiento (Lacasa A, Guerrero MM, Oncina M, Mora JA, eds.). Publicaciones de la Consejería de Agricultura, Agua y Medio Ambiente, Región de Murcia (Spain), pp: 208-238. 

Guerrero MM, Ros C, Martínez MA, Barceló N, Martínez MC, Guirao P, Bello A, Contreras J, Lacasa A, 2004b. Estabilidad en la eficacia desinfectante de la biofumigación con solarización en cultivos de pimiento. Acta Hort 42: 20-24. 

Guerrero MM, Ros C, Martínez MA, Martínez MC, Bello A, Lacasa A, 2006. Biofumigation vs biofumigation plus solarization to control Meloidogyne incognita in sweet pepper. IOBC-WPRS Bulletin 29: 313-318. 

Guerrero MM, Martínez MA, Ros C, Bello A, Fernández P, Martínez MC, Lacasa A, 2007. Eficacia de la biosolarización como desinfectante del suelo en invernaderos de pimiento. Acta Hort 48: 451-454. 

Guerrero MM, Lacasa CM, Ros C, Martínez V, Fenoll J, Torres J, Beltrán C, Fernández P, Bello A, Lacasa A, 2009. Pellets de brasicas como enmiendas para biosolarización de invernaderos de pimiento. Acta Hort 54: 424-429. 

Guerrero MM, Ros C, Lacasa C, Martínez V, Lacasa A, Fernández P, Martínez MA, Nú-ez M, Larregla S, Díez-Rojo MA, Bello A, 2010. Effect of biosolarization using pellets of Brassica carinata on soil-borne pathogens in protected pepper crops. Acta Hortic 381: 337-344. 

Guerrero MM, Lacasa CM, Hernández A, Martínez V, Martínez MA, Ros C, 2012. Biosolarización e injerto para el manejo integrado de los patógenos del suelo en cultivos de pimiento en invernadero. Acta Hort 60: 321-326. 

Heald CD, Robinson AF, 1987. Effect of soil solarization on Rotylenchuslus reniformis in the lower Rio Grande Valley of Texas. J Nematol 19: 93-103.
PMid:19290112 PMCid:2618608 

Hewlet TE, Dickson DW, 1991. Population dynamics of Meloidogyne arenaria race 1 in peanut. J Nematol 25: 532-533. 

Katan J, 1981. Solar heating (solarization) of soil control of soilborne pets. Annu Rev Phytopathol 19: 211-236.
http://dx.doi.org/10.1146/annurev.py.19.090181.001235 

Katan J, 2005. Soil disinfestation: one before methyl bromide phase out. Acta Hortic 698: 19-25. 

Katase M, Kubo C, Ushio S, Ootsuka E, Takeuchi T, Mizukubo T, 2009. Nematicidal activity of volatile fatty acids generated from wheat bran in reductive soil disinfestation. J Nematol 39: 53-62.
http://dx.doi.org/10.3725/jjn.39.53 

Kirkegaard JA, Sarwar M, 1998. Biofumigation potential of brassicas - I. Variation in glucosinolate profiles of diverse field-grown brassicas. Plant Soil 201: 71-89.
http://dx.doi.org/10.1023/A:1004364713152 

Klein E, Katan J, Gamliel A, 2011. Soil suppressiveness to Fusarium dissease following organic amendments and solarization. Plant Dis 95: 1116-1123.
http://dx.doi.org/10.1094/PDIS-01-11-0065 

Lacasa A, Guirao P, 1997. Investigaciones actuales sobre alternativas al uso del bromuro de metilo en pimiento en invernaderos del campo de Cartagena. In: Posibilidades de alternativas viables al bromuro de metilo en pimiento en invernadero (López A, Mora JA, eds.). Jornadas 11, Publicaciones de la Consejería Medio Ambiente, Agricultura y Agua. Región de Murcia (Spain), pp: 21-36.

 

Larkin RP, Griffin TS, 2008. Control of soil potato diseases using Brassica green manure. Crop Prot 26: 1067-1077.
http://dx.doi.org/10.1016/j.cropro.2006.10.004 

Lazarovits G, 2001. Management of soil-borne plant pathogens with organic soil amendments: a disease control strategy salvaged from the past. Can J Plant Pathol 23: 1-7.
http://dx.doi.org/10.1080/07060660109506901 

Lazzeri L, Manici LM, 2000. The glucosinolate-myrosinase system: a natural and practical tool for biofumigation. Acta Hort 532: 89-95. 

Lazzeri L, Leoni O, Manici LM, 2004. Biocidal plant dried pellets for biofumigation. Ind Crop Prod 20: 59-65.
http://dx.doi.org/10.1016/j.indcrop.2003.12.018 

Lazzeri L, Leoni O, Mancini L, Palmieri S, Patalano G, 2010. Use of seed flour as soil pesticide. Patent number US7749549B2.

 

Martínez MA, Martínez MC, Bielza P, Tello J, Lacasa A, 2011. Effect of biofumigation with manure amendments and repeated biosolarization on Fusarium density in pepper crops. J Ind Microbiol Biot 38(10): 3-11.
http://dx.doi.org/10.1007/s10295-010-0826-2
PMid:20820866  

Matthiessen J, 2004. The importance of plant maceration and water in achieving high ITC levels in soil. Proc First Int Symp on Biofumigation: A Possible Alternative to Methylbromide? (Lazzeri L, Matthiessen J, Morra M J, Palmieri S, Rollin P, eds.). Italian Ministry of Agricultural and Forestry Politics and Italian Ministry of Environmental and Territory, p: 13.

 

Messiha N, Van Diepeningen A, Wenneker M, Van Beuningen A, Janse J, Coehen T, Termorshuizen A, Van Bruggen A, Blok W, 2007. Biological soil disinfestation (BSD), a new control method for potato brown rot, caused by Ralstonia solanacearum race 3 biovar 2. Eur J Plant Pathol 117: 403-415.
http://dx.doi.org/10.1007/s10658-007-9109-9 

Morra MJ, 2004. Controlling soil-borne plant pests using glucosinolate containing tissues. Proc First Int Symp on Biofumigation: A Possible Alternative to Methylbromide? (Lazzeri L, Matthiessen J, Morra M J, Palmieri S, Rollin P, eds.). Italian Ministry of Agricultural and Forestry Politics and Italian Ministry of Environmental and Territory, p: 7.

 

Nú-ez-Zofío M, Larregla S, Garbisu C, 2011. Application of organic amendments followed by soil plastic mulching reduces the incidence of Phytophthora capsici in pepper crops under temperate climate. Crop Prot 30: 1563-1572.
http://dx.doi.org/10.1016/j.cropro.2011.08.020 

Nu-ez-Zofío M, 2012. Evaluación de la biodesinfección para el control de Phytophthora capsici en el cultivo de pimiento en invernadero. Doctoral thesis. Univ. del País Vasco, Spain.

 

Oka Y, 2010. Mechanisms of nematode suppression by organic soil amendments: a review. Appl Soil Ecol 44: 101-115.
http://dx.doi.org/10.1016/j.apsoil.2009.11.003 

Palmieri S, 2005. Biofumigation: a new technology based on the use of sulphur-containing metabolites in Brassica plants. Proc Annual Meeting Assoc Advancement of Industrial Crops, Murcia (Spain), Sept 17-21. pp: 201-213. 

Pikerton JN, Ivors KL, Miller ML, Moore LW, 2000. Effect of soil solarization and cover crops on populations of selected soilborne plant pathogens in Western Oregon. Plant Dis 84: 952-960.
http://dx.doi.org/10.1094/PDIS.2000.84.9.952 

Riegel C, Noe JP, 2000. Chicken litter soil amendment effects on soilborne microbes and Meloidogyne incognita on cotton. Plant Dis 84: 1275-1281.
http://dx.doi.org/10.1094/PDIS.2000.84.12.1275 

Robertson L, López-Pérez JA, Bello A, Díez-Rojo MA, Escuer M, Piedra-Buena A, Ros C, Martínez C, 2006. Characterization of Meloidogyne incognita, M. arenaria and M. hapla populations from Spain and Uruguay parasitizing pepper (Capsicum annuum). Crop Prot 25: 440-445.
http://dx.doi.org/10.1016/j.cropro.2005.07.008 

Ros M, García C, Hernández MT, Lacasa A, Fernández P, Pascual JA, 2008. Effects of biosolarization as methyl bromide alternative for Meloidogyne incognita control on quality of soil under pepper. Biol Fertil Soils 45: 37-44.
http://dx.doi.org/10.1007/s00374-008-0307-1 

Sachi S, Odorizzi S, Lazzeri L, Marciano P, 2005. Effect of Brassica carinata seed meal treatment on the Trichoderma harzianum T39-Sclerotinia species interaction. Acta Hort 698: 289-292. 

Smolinska U, Morra MJ, Kundsen GR, Brown PD, 1997. Toxicity of glucosinolate degradation products from Brassica napus seed meal toward Aphanomyces euteiches f. sp. pisi. Phytopathology 87: 77-82.
http://dx.doi.org/10.1094/PHYTO.1997.87.1.77
PMid:18945157  

Smolinska U, Morra MJ, Kanudsen GR, James RL, 2003. Isothiocyanate produced by Brassicaceae species as inhibitors of Fusarium oxysporum. Plant Dis 87: 407-412.
http://dx.doi.org/10.1094/PDIS.2003.87.4.407 

Stapleton J, Ba-uelos GS, 2009. Biomass crops can be used for biological disinfestation and remediation of soil and water. Calif Agr 63: 41-46.
http://dx.doi.org/10.3733/ca.v063n01p41 

Tello J, Lacasa A, 1997. Problemática fitosanitaria del suelo en cultivos de pimiento en el Campo de Cartagena. In: Posibilidades de alternativas viables al bromuro de metilo en pimiento en invernadero (López A, Mora JA, eds.). Jornadas 11, Publicaciones de la Consejería Medio Ambiente, Agricultura y Agua, Región de Murcia (Spain): 11-17.

 

Tenuta M, Lazarovits G, 2002. Ammonia and nitrous acid from nitrogenous amendments kill the microsclerotia of Verticillium dahliae. Phytopathology 92: 255-264.
http://dx.doi.org/10.1094/PHYTO.2002.92.3.255
PMid:18943996  

Tenuta M, Conn KL, Lazarovits G, 2002. Volatile fatty in liquid swine manure can kill microsclerotia of Verticillium dahliae. Phytopathology 92: 548-552.
http://dx.doi.org/10.1094/PHYTO.2002.92.5.548
PMid:18943030  

Wang KH, McSorley R, 2008. Exposure time to lethal temperatures for Meloidogyne incogita suppression and its implication for soil solarisation. J Nematol 40 (1): 7-12.
PMid:19259512 PMCid:2586528 

Wang KH, McSorley R, Kokalis-Burelle N, 2006. Effects of cover cropping solarization, and soil fumigation on nematode comunities. Plant Soil 286: 299-243.
http://dx.doi.org/10.1007/s11104-006-9040-4 

Yossen V, Zumelza G, Gasoni L, Kobayashi K, 2008. Effect of soil reductive sterilization on Fusarium wilt in greenhouse carnation in Cordoba, Argentina. Australas Plant Path 37: 520-522.
http://dx.doi.org/10.1071/AP08039 

Zasada IA, Meyer SLF, Morra MJ, 2009. Brassicaceous seed meals as soil amendments to suppress the plant-parasitic nematodes Pratylenchus penetrans and Meloidogyne incognita. J Nematol 41(3): 221-227.
PMid:22736818 PMCid:3380494 

Zasada IA, Halbrent JM, Kokalis-Burelle N, La Mondia J, McKerny MV, Noling JW, 2010. Managing nematodes without methyl bromide. Ann Rev Phytopathol 48: 311-328.
http://dx.doi.org/10.1146/annurev-phyto-073009-114425
PMid:20455696

Published
2013-04-01
How to Cite
Guerrero-Díaz, M. M., Lacasa-Martínez, C. M., Hernández-Piñera, A., Martínez-Alarcón, V., & Lacasa-Plasencia, A. (2013). Evaluation of repeated biodisinfestation using Brassica carinata pellets to control Meloidogyne incognita in protected pepper crops. Spanish Journal of Agricultural Research, 11(2), 485-493. https://doi.org/10.5424/sjar/2013112-3275
Section
Plant protection