Short communication. Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of Quillaja saponaria-A and extracts of Cochinchina momordica seed

C. W. Xiao, Z. I. Rajput, G. L. Bao, S. H. Hu, N. A. Soomro

Abstract


This study was designed to evaluate the effects of oral administration of extracts from Cochinchina momordica seed (ECMS) or Quillaja saponaria-A (Quil-A) on the immune responses in mice immunized with foot and mouth disease virus (FMDV)-serotype O vaccine. Forty-two imprinting control region (ICR) mice were randomly divided into seven groups of 6 animals in each group, and a dose of 400 μg of Quil-A or ECMS was orally administered for 1,, 2 or 3 days. After that, the animals were subcutaneously immunized twice with FMD vaccine at 3-week intervals and blood samples were collected 2-weeks after boosting for measurement of FMDV-specific IgG and its subclasses. Spleens were collected for lymphocytes proliferation assay. Results indicated that serum FMDV-specific IgG and the IgG subclass responses were significantly enhanced in mice orally administered ECMS or Quil-A when compared with the control group (p<0.05). Lymphocytes proliferation response to FMD vaccine was significantly enhanced by ECMS compared with the control (p<0.05). This study illustrates that ECMS induced immunomodulatory effects and performed better than Quil-A.

Keywords


adjuvant; ELISA; oral immunization; vaccine

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References


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DOI: 10.5424/sjar/2013111-3279