Short communication. First report of Eggplant mottled dwarf virus in China rose in southern Spain

G. Parrella, A. De Stradis, B. Greco, F. Villanueva, I. M. Fortes, J. Navas-Castillo

Abstract


Eggplant mottled dwarf virus (EMDV, genus Nucleorhabdovirus, family Rhabdoviridae) is transmitted in nature by leafhoppers and its natural host range includes vegetable crops (eggplant, tomato, potato, pepper), ornamentals (pittosporum, honeysuckle, pelargonium) and wild plants (caper, Solanum nigrum). The prevalence of infections is generally very low. EMDV has been demonstrated to be the causal agent of a vein yellowing disease of China rose (Hibiscus rosa-sinensis) in southern Italy. In this work, four locations from Málaga and Granada provinces (southern Spain) were surveyed in 2011 to study the prevalence of EMDV infections in China rose by serological and molecular methods. Overall, EMDV was detected in 77.3% of the samples (33 out of 45 samples tested). Mechanical transmission tests and immunoelectron microscopy confirmed the presence of EMDV. The possible causes of such a high and unexpected prevalence are discussed. The use of molecular hibridization with an EMDV specific riboprobe is proposed for early screening of vegetative propagated China rose plants to avoid dissemination of infected material.

Keywords


Hibiscus rosa-sinensis; EMDV; Nucleorhabdovirus; molecular probe; ELISA

Full Text:

PDF

References


Alfaro-Fernández A, Córdoba-Sellés C, Tornos T, Cebrián MC, Font MI, 2011. First report of Eggplant mottled dwarf virus in Pittosporum tobira in Spain. Plant Dis 95: 75.
http://dx.doi.org/10.1094/PDIS-07-10-0491 

Al-Musa AM, Lockhart B, 1990. Occurrence of Eggplant mottled dwarf virus in Jordan. J Phytopathol 128: 283-287.
http://dx.doi.org/10.1111/j.1439-0434.1990.tb04275.x 

Aramburu J, Galipienso L, Tornos T, Matas M, 2006. First report of Eggplant mottled dwarf virus in mainland Spain. Plant Pathol 55: 565.
http://dx.doi.org/10.1111/j.1365-3059.2006.01419.x 

Babaie GH, Izadpanah K, 2003. Vector transmission of Eggplant mottled dwarf virus in Iran. J Phytopathol 151: 679-682.
http://dx.doi.org/10.1046/j.1439-0434.2003.00788.x 

Della Giustina W, Javoy M, Bansept P, Morel E, Balasse H, Goussard N, Passard C, 2000. Les cidadelles du genre Anacertagallia vectrice du virus responsable de la maladie de la peau de crapaud du concombre. PHM Rev Hort 420: 40-43. 

De Stradis A, Parrella G, Vovlas C, Ragozzino A, 2008. Vein yellowing of Hibiscus rosa-sinensis caused by Eggplant mottled dwarf virus in Southern Italy. J Plant Pathol 90: 359-361. 

Lockhart BEL, 1987. Evidence for identity of plant rhabdoviruses causing vein-yellowing disease of tomato and Hibiscus rosa-sinensis. Plant Dis 71: 731-733.
http://dx.doi.org/10.1094/PD-71-0731 

Martelli GP, Hamadi A, 1986. Occurrence of Eggplant mottled dwarf virus in Algeria. Plant Pathol 35: 595-597.
http://dx.doi.org/10.1111/j.1365-3059.1986.tb02061.x 

Martelli GP, Russo M, Rubino L, 2011. Eggplant mottle dwarf virus. In: Description of plant viruses, No 421, Association of Applied Biologist. Available online in http://www.dpvweb.net/dpv/showdpv.php?dpvno=421. [20 August 2012].

 

Milne RG, Luisoni E, 1977. Rapid immune electron microscopy of virus preparations. In: Methods in Virology (Maramorsch K, Koprowski H, eds). Academic Press, NY. Vol. 6, pp: 265-281. 

Parrella G, Moretti A, Gognalons P, Lesage ML, Marchoux G, Gebre-Selassie K, Caranta C, 2004. The Am gene controlling resistance to Alfalfa mosaic virus in tomato is located in the cluster of dominant resistance genes on chromosome 6. Phytopathology 94: 345-350.
http://dx.doi.org/10.1094/PHYTO.2004.94.4.345
PMid:18944110  

Parrella G, Fiallo-Olivé E, Navas-Castillo J, 2012. First report of China rose (Hibiscus rosa-sinensis) as a host of Alfalfa mosaic virus in Spain. Plant Dis 96: 462.
http://dx.doi.org/10.1094/PDIS-09-11-0781 

Roggero P, Milne RG, Masenga V, Ogliara P, Stravato VM, 1995. First reports of eggplant mottled dwarf rhabdovirus in cucumber and pepper. Plant Dis 79: 321.
http://dx.doi.org/10.1094/PD-79-0321C 

Tordo N, Benmansour A, Calisher C, Dietzgen RG, Fang R-X, Jackson AO, Kurath G, Nadin-Davis S, Tesh RB, Walker PJ, 2005. Family Rhabdoviridae. In: Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses (Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, eds). Elsevier/Academic, London. pp: 623–644.




DOI: 10.5424/sjar/2013111-3461