A comparison of controlled self-pollination and open pollination results based on maize grain quality

Hanna Sulewska, Jozef Adamczyk, Hennryk Cygert, Janusz Rogacki, Grazyna Szymanska, Karolina Smiatacz, Katarzyna Panasiewicz, Kamila Tomaszyk


Maize (Zea mays L.) grain endosperm is triploid (3n), of which 2n come from the male (transferred by pollen) and only 1n from the female plant, thus a major impact of the male form can be expected on grain quality parameters. A good example of this relationship is the phenomenon of xenia. The aim of this study was to determine the effect of pollen on grain quality. The field experiment was conducted in 2011; seeds were harvested from eight cultivars: Bosman, Blask, Tur, Kozak, Bielik, Smok, SMH 220 and Kresowiak, derived from free pollination and controlled self-pollination of maize. Analyses of nutrient contents and starch content in the grain were conducted in the laboratory. In addition, 1000 grain weight and the hectoliter weight of all grain samples were recorded. The results confirmed differences in grain quality of maize hybrids obtained by self-pollination and by open pollination. Grain of maize plants obtained by open-pollination was characterised by higher contents of N-free extract and starch, and lower protein content. Undertaking further studies on this subject may indicate specific recommendations for agricultural practice, such as mixtures of hybrids with good combining abilities, which will contribute to improved grain quality without additional costs.


chemical composition; hybrids; pollen; pollination

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Adamczyk J, Rogacki J, Cygert H, 2010. The progress in maize breeding in Poland. Acta Sci Pol, Agricultura 5(4): 85-91 [in Polish].

Andrade JAC, Pereira FCD, 2005. Uso do efeito de xenia em hibridos comerciais de milho (Zea mays L.) Revista Brasileira de Milho e Sorgo 4(1): 65-78.

Arritt RW, Clark CA, Goggi SA, Lopez Sanchez H, Westgate ME, Riese JM, 2007. Lagrangian numerical simulations of canopy air flow effects on maize pollen dispersal. Field Crops Res 102: 151-162. http://dx.doi.org/10.1016/j.fcr.2007.03.008

Balestre M, Souza JC, Luders RR, ASilva NO, 2007a. Effect of allelopollen in artificial crosses of white and yellow endosperm maize hybrids. Crop Breeding Appl Biotechnol 7: 82-87. http://dx.doi.org/10.12702/1984-7033.v07n01a12

Balestre M, Souza JC, Santos JB, Luders RR, Lima JL, 2007b. Effect of self-pollination monitored by microsatellite markers on maize kernel weight. Crop Breeding Appl Biotechnol 7: 340-344. http://dx.doi.org/10.12702/1984-7033.v07n04a02

Brookes G, Barfoot P, Melè E, Messeguer J, Bènètrix F, Bloc D, Foueillassar X, Fabiè A, Poeydomenge C, 2004. Genetically modified maize: pollen movement and crop co-existence. PG Economics: 1–20. Available at http://www.pgeconomics.co.uk. [25 June 2013].

Bulant C, Gallais A, 1998. Xenia effects in maize with normal endosperm: I. Importance and stability. Crop Sci 39: 1517-1525. http://dx.doi.org/10.2135/cropsci1998.0011183X003800060019x

Bulant C, Gallais A, Matthys-Rochon E, Prioul JL, 2000. Xenia effects in maize with normal endosperm: II. Kernel growth and enzyme activities during grain filling. Crop Sci 40: 182-189. http://dx.doi.org/10.2135/cropsci2000.401182x

Chavez DJ, Lyrene PM, 2009. Effect of self-pollination and cross-pollination of Vaccinium darrowii (Ericaceae) and other low-chill blueberries. HortScience 44(6): 1538-1541.

CSO, 2012. Statistical Yearbook of the Republic of Poland. Available at http://www.stat.gov.pl/cps/rde/xbcr/gus/RL_przedwynik_szac_glow_ziemioplod_rol_i_ogrodniczych_2012.pdf. [25 June 2013].

CSO, 2012. Statistical Yearbook of the Republic of Poland. Available at http://www.stat.gov.pl/cps/rde/xbcr/gus/RL_przedwynik_szac_glow_ziemioplod_rol_i_ogrodniczych_2012.pdf. [25 June 2013].

Davarynejad GH, Nyéki J, Hámori Szabó J, Lakier Z, 1994. Relationship between pollen donors and quality of fruits of 12 apple cultivars. Int Symp on Postharvest Treatment of Horticultural Crops. Acta Horticultura, Keeskemet: 344-354.

David O, Monod H, Lorgeou J, Philippeau G, 2001. Control of interplot interference in grain maize: a multi-site comparison. Crop Sci 41: 406-414. http://dx.doi.org/10.2135/cropsci2001.412406x

Denney J, 1992. Xenia includes metaxenia. Hort Science 27: 722-728.

Derin K, Eti S, 2001. Determination of pollen quality, quantity and effect of cross pollination on the fruit set and quality in the pomegranate. Turk J Agric For 25: 169-173.

Dicenta F, Ortega E, Canovas JA, Egea J, 2002. Self-pollination vs. cross-pollination in almond: pollen tube growth, fruit set and fruit characteristics. Plant Breeding 121: 163-167. http://dx.doi.org/10.1046/j.1439-0523.2002.00689.x

Felsot AS, 2002. Some corny ideas about gene flow and biodiversity. Agrichemical and Environmental News 193(13). Available in http://aenews.wsu.edu. [30 June 2013].

Gugała M, 2002. Natural starch – Determination of starch content by polarimetric method. Polish Standard No. PN–EN ISO 10520:2002, Committee for Standardization, Poland. 13 pp. [in Polish]

Hoekstra GJ, Kannenberg LW, Christie BR, 1985. Grain yield comparison of pure stands and mixture of different proportions for two hybrids of maize. Can J Plant Sci 65: 481-485. http://dx.doi.org/10.4141/cjps85-069

Idikut L, Atala AI, Kara SN, Kamalak A, 2009. Effect of hybrid on starch, protein and yields of maize grain. J Anim Vet Adv 8(10): 1945-1947.

Leng ER, 1949. Direct effect of pollen parent on kernel size in dent corn. Agron J 41: 555-558. http://dx.doi.org/10.2134/agronj1949.00021962004100120003x

Letchworth MB, Lamber RJ, 1998. Pollen parent effects on oil, protein and starch concentration in maize kernels. Crop Sci 38(2): 363-367. http://dx.doi.org/10.2135/cropsci1998.0011183X003800020015x

Lopes MA, Larkins BA, 1993. Endosperm origin development and function. The Plant Cell 5: 1383-1399. http://dx.doi.org/10.1105/tpc.5.10.1383

Marek T, 1989. Cluster analysis in empirical research. SAHN methods. PWN Warsaw [in Polish].

Mercer J, Ramalho MAP, Raposo FV, 2002. Implicações do fenõmeno xênia nos programas de melhoramento de milho. Ciência e Agrotecnologia 26: 1338-1343.

Morrison DF, 1990. Multivariate statistical methods. McGraw-Hill, NY.

Nderitu J, Nyamasyo G, Kasina M, Oronje ML, 2008. Diversity of sunflower pollinators and their effect on seed yield in Makueni District, Eastern Kenya. Span J Agric Res 6(2): 271-278. http://dx.doi.org/10.5424/sjar/2008062-318

Oukabli A, Lansari A, Walali-Loudiyi DE, Abousalim A, 2002. Effects of controlled self-pollination and cross-pollination on fruit set, embryo viability and pomological traits in the self-compatible almond cv 'Tuono'. Acta Hort 591: 429-435.

Persson JA, 2008. Handbook for Kjeldahl digestion. FOSS, Denmark. 84 pp.

Pinter L, Szabo J, Horompoli E, 1987. Effect of metaxenia on the grain weight of corn (Zea mays L.). Maydica 32: 81-88.

Seka D, Cross HZ, 1995. Xenia and maternal effects on maize agronomic traits at three plant densities. Crop Sci 35: 86-90. http://dx.doi.org/10.2135/cropsci1995.0011183X003500010015x

Seka D, Cross HZ, McClean PE, 1995. Maize kernel development in vitro: sucrose concentration, xenia and material effects. Crop Sci 35: 74-79. http://dx.doi.org/10.2135/cropsci1995.0011183X003500010013x

Tollenar M, Lee EA, 2006. Dissection of physiological processes underlying grain yield in maize by examining genetic improvement and heterosis. Maydica 51: 399-408.

Tsai CI, Tsai CY, 1990. Endosperm modified by cross-pollinating maize to induce changes in dry matter and nitrogen accumulation. Crop Sci 30: 804-808. http://dx.doi.org/10.2135/cropsci1990.0011183X003000040008x

Virot M, Tomao V, Ginies C, Visinoni F, Chemat F, 2008. Microwave-integrated extraction of total fats and oils. J Chromatogr A 1196–1197: 57-64. http://dx.doi.org/10.1016/j.chroma.2008.05.023

Weiland RT, 1992. Cross-pollination effects on maize (Zea mays L.) hybrid yields. Can J Plant Sci 72: 27-33. http://dx.doi.org/10.4141/cjps92-004

Zilic S, Milasinovic M, Terzic D, Barac M, Ignjatovic-Micic D, 2011. Grain characteristic and composition of maize specialty hybrids. Span J Agric Res 9(1): 230-241. http://dx.doi.org/10.5424/sjar/20110901-053-10

DOI: 10.5424/sjar/2014122-4970