Short communication: The effect of different inclusion levels of polyethylene glycol as a silage additive on ensilage characteristics of pomegranate peel and in vitro rumen fermentation

  • Ali Hatami Bu-Ali Sina University, Faculty of Agriculture, Dept. Animal Science. Hamedan.
  • Daryoush Alipour Bu-Ali Sina University, Faculty of Agriculture, Dept. Animal Science. Hamedan.
  • Fardin Hozhabri Razi University, Faculty of Agriculture, Dept. Animal Science. Kermanshah
  • Meisam Tabatabaei Biofuel Research Team (BR Team)/Agricultural Biotechnology Research Institute of Iran (ABRII), Microbial Biotechnology and Biosafety Department. Karaj
Keywords: Punica granatum, agro-industrial by-products, tannin, microbial biomass, lactic acid, partitioning factor

Abstract

This study was conducted to evaluate the effects of ensiling pomegranate peel (PP) with different levels of polyethylene glycol (PEG) on its chemical composition, tannin content, in vitro gas production and fermentation characteristics. Fresh PP was chopped and ensiled in mini silos made of polyvinyl chloride tubing. Five levels of PEG were studied: 0 (control), 5, 10, 15, and 20% of fresh PP (dry matter basis). Total phenolics, total tannins, crude ash, crude protein, neutral detergent fiber and acid detergent fiber content and pH decreased with increasing PEG levels, whereas dry matter and non-fiber carbohydrates content, non-tannin phenols, lactic acid and ammonia concentrations and buffering capacity increased. The water soluble carbohydrates and ether extract concentrations were not influenced by the addition of PEG. The partitioning factor and efficiency of microbial biomass production were quadratically decreased (p=0.020 and p=0.032, respectively) as PEG inclusion increased, but the in vitro apparent dry matter disappearance did not differ among treatments. Compared to control, the in vitro true disappearance and in vitro fiber digestibility had a tendency to be higher in silages treated with PEG (p=0.081 and p=0.069, respectively). The metabolizable energy content and total volatile fatty acids concentration increased quadratically by PEG inclusion. The asymptotic gas production and rate of gas production were higher in PEG-treated silages. Overall, ensiling PP with PEG can improve the fermentation characteristics of this by-product.

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References

Alipour D, Rouzbehan Y, 2007. Effects of ensiling grape pomace and addition of polyethylene glycol on in vitro gas production and microbial biomass yield. Anim Feed Sci Technol 137(1): 138-149. http://dx.doi.org/10.1016/j.anifeedsci.2006.09.020

AOAC, 1990. Official methods of analysis, vol. II, 15th ed. Association of Official Analytical Chemists, Arlington, VA, USA.

Baba ASH, Castro FB, Ørskov ER, 2002. Partitioning of energy and degradability of browse plants in vitro and the implications of blocking the effects of tannin by the addition of polyethylene glycol. Anim Feed Sci Technol 95: 93-104. http://dx.doi.org/10.1016/S0377-8401(01)00283-8

Barnett AG, Reid RL, 1957. Studies on production of volatile fatty acid production from fresh grass. J Agric Sci Camb 48: 315. http://dx.doi.org/10.1017/S0021859600031671

Belenguer A, Hervás G, Toral PG, Fondevila M, Frutos P, 2011. Is polyethylene glycol innocuous to the rumen bacterial community? A preliminary in vitro study. Anim Prod Sci 51: 990-995. http://dx.doi.org/10.1071/AN11041

Bento MHL, Makkar HPS, Acamovic T, 2005. Effect of mimosa tannin and pectin on microbial protein synthesis and gas production during in vitro fermentation of 15N-labelled maize shoots. Anim Feed Sci Technol 123/124: 365-377. http://dx.doi.org/10.1016/j.anifeedsci.2005.04.022

Blümmel M, 2000. Predicting the partitioning of fermentation products by combined in vitro gas volume-substrate degradability measurements: opportunities and limitations. In: Gas production: fermentation kinetics for feed evaluation and to assess microbial activity. Brit Soc Anim Sci, Penicuik, Midlothian, pp: 48-58.

Blümmel M, Makkar HPS, Becker K, 1997. In vitro gas production: a technique revisited. J Anim Physiol Anim Nutr 77: 24-34. http://dx.doi.org/10.1111/j.1439-0396.1997.tb00734.x

Broderick GA, Kang JH, 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 63: 64-75. http://dx.doi.org/10.3168/jds.S0022-0302(80)82888-8

Faithfull NT, 2002. Methods in agricultural chemical analysis: a practical handbook. CABI Publ, UK, 266 pp. http://dx.doi.org/10.1079/9780851996080.0000

France J, Dijkstra J, Dhanoa MS, Lopez S, Bannink A, 2000. Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations. Br J Nutr 83:143-150. http://dx.doi.org/10.1017/S0007114500000180

Frutos P, Hervas G, Giráldez F J, Mantecón AR, 2004. An in vitro study on the ability of polyethylene glycol to inhibit the effect of quebracho tannins and tannic acid on rumen fermentation in sheep, goats, cows and deer. Aust J Agric Res 55(11): 1125-1132. http://dx.doi.org/10.1071/AR04058

Makkar HPS (Ed.), 2000. Quantification of tannins in tree foliage. A laboratory manual for the FAO/IAEA co-ordinated research project on use of nuclear and related techniques to develop simple tannin assays for predicting and improving the safety and efficiency of feeding ruminants on tanniniferous tree foliage. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Animal Production and Health Sub-Programme, FAO/IAEA Working Document, IAEA, Vienna, Austria.

Makkar HPS, 2003. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Rumin Res 49: 41-256. http://dx.doi.org/10.1016/S0921-4488(03)00142-1

Makkar HPS, 2005. In vitro gas methods for evaluation of feeds containing phytochemicals. Anim Feed Sci Technol 123/124: 291-302. http://dx.doi.org/10.1016/j.anifeedsci.2005.06.003

Makkar HPS, Blümmel M, Becker, K, 1998. Application of an in vitro gas method to understand the effects of natural plant products on availability and partitioning of nutrients. Br Soc Anim Sci 22:147-150.

Mangan JL, 1988. Nutritional effects of tannins in animal feeds. Nutr Res Rev 1: 209-231. http://dx.doi.org/10.1079/NRR19880015

Markham R, 1942. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem J 36:790.

Menke KH, Steingass H, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev 28: 7-55.

Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W, 1979. The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Food Sci 93: 217-222.

Moharrery A, 2007. The determination of buffering capacity of some ruminant's feedstuffs and their cumulative effects on TMR ration. Am J Anim Vet Sci 2(4): 72-78. http://dx.doi.org/10.3844/ajavsp.2007.72.78

Negro C, Tommasi L, Miceli A, 2003. Phenolic compounds and antioxidant activity from red grape marc extracts. Biores Technol 87(1): 41-44. http://dx.doi.org/10.1016/S0960-8524(02)00202-X

NRC, 2001. Nutrient requirements of dairy cattle, 7th rev ed. Nat Acad Press, Washington, D.C., USA.

Rozes N, Peres C, 1998. Effects of phenolic compounds on the growth and the fatty acid composition of Lactobacillus plantarum. Appl Microbiol Biotech 49(1): 108-111. http://dx.doi.org/10.1007/s002530051145

Salawu MB, Acamovic T, Stewart CS, Hvelplund T, Weisbjerg MR, 1999. The use of tannins as silage additive: effect on silage composition and mobile bag disappearance of dry matter and protein. Anim Feed Sci Technol 82: 243-259. http://dx.doi.org/10.1016/S0377-8401(99)00105-4

SAS, 2004.User's guide, V. 9.1: Statistics. SAS Institute, Cary, NC, USA.

Shabtay A, Eitam H, Tadmor Y, Orlov A, Meir A, Weinberg P, Weinberg ZG, Chen Y, Brosh A, Izhaki I, Kerem Z, 2008. Nutritive and antioxidative potential of fresh and stored pomegranate industrial byproduct as a novel beef cattle feed. J Agric Food Chem 56: 10063-10070. http://dx.doi.org/10.1021/jf8016095

Taghavi-Nezhad M, Alipour D,Torabi Goudarzi M, Zamani P, Khodakaramian G, 2011. Dose response to carvone rich essential oils of spearmint (Mentha spicata L.): in vitro ruminal fermentation kinetics and digestibility. J Agr Sci Tech 13: 1013-1020.

Taylor KA, 1996.A simple colorimetric assay for muramic acid and lactic acid. Appl Biochem Biotechnol 561: 49-58. http://dx.doi.org/10.1007/BF02787869

Van Soest, PJ, Robertson, JB, Lewis BA, 1991. Methods for dietary fiber, neutral detergent fiber and non-starch carbohydrates in relation to animal nutrition. J Dairy Sci 74: 3583-3597. http://dx.doi.org/10.3168/jds.S0022-0302(91)78551-2

Published
2015-05-29
How to Cite
Hatami, A., Alipour, D., Hozhabri, F., & Tabatabaei, M. (2015). Short communication: The effect of different inclusion levels of polyethylene glycol as a silage additive on ensilage characteristics of pomegranate peel and in vitro rumen fermentation. Spanish Journal of Agricultural Research, 13(2), e06SC01. https://doi.org/10.5424/sjar/2015132-6463
Section
Animal production