Short Communication. Embryo yield in llamas synchronized with two different intravaginal progesterone-releasing devices and superovulated with eCG

Juan F. Aller, Marcos C. Abalos, Francisco A. Acuña, Rosana Virgili, Francisco Requena, Andrea K. Cancino

Abstract


The objectives of this study were to compare the effects of two intravaginal devices (ID) containing the same dose (0.5 g) of progesterone (P4) on subsequent ovarian response, embryo production and circulating P4 concentration profile in llamas (Lama glama) treated with equine chorionic gonadotropin (eCG) for ovarian superstimulation. Female llamas were randomly assigned (n = 10 llamas per group) to one of the following groups and treated (Day 0) with an ID containing 0.5 g of vegetal P4 to synchronize the emergence of a new follicular wave: i) DIB 0.5® and ii) Cronipres M15®. On Day 3 llamas were intramuscularly treated with 1000 IU of eCG. The IDs were removed on Day 7. Llamas were naturally mated (Day 9) and treated with GnRH analogue to induce ovulation. A second mating was allowed 24 h later. Embryos were collected between 7 and 8 days after the first mating. Blood samples were taken every day from Day 0 to Day 7 to measure circulating P4 concentrations. The results indicated that DIB device maintained greater plasma P4 levels as compared to Cronipres until Day 2. However, the mean (± SD) number of corpora lutea and recovered embryos was not affected (p < 0.05) by the type of ID (5.3 ± 2.6 vs 4.2 ± 2.2 and 3.5 ± 2.7 vs 2.6 ± 3.0 for DIB and Cronipres, respectively). In conclusion, both DIB and Cronipres devices can be successfully used to synchronize the emergence of follicular wave prior to a single dose of eCG in superovulation protocol in llamas.


Keywords


South American camelids; superovulation; hormonal treatment; embryo

Full Text:

PDF HTML XML

References


Aba MA, Quiroga MA, Auza N, Forsberg M, Kindahl, H, 1999. Control of ovarian activity in llamas (Lama glama) with medroxiprogesterone acetate. Reprod Dom Anim 34: 471-476. http://dx.doi.org/10.1111/j.1439-0531.1999.tb01406.x

Adams GP, Nasser LF, Bo GA, Garcia A, del Campo, MR, Mapletoft RJ, 1994. Superovulatory response of ovarian follicles of wave 1 versus wave 2 in heifers. Theriogenology 42: 1103-1113. http://dx.doi.org/10.1016/0093-691X(94)90858-3

Aller JF, Rebuffi GE, Cancino AK, Alberio RH, 2002. Successful transfer of vitrified llama (Lama glama) embryos. Anim Reprod Sci 73: 121-127. http://dx.doi.org/10.1016/S0378-4320(02)00120-3

Aller JF, Cancino AK, Rebuffi GE, Alberio RH, 2010. Effect of estradiol benzoate used at the start of a progestagen treatment on superovulatory response and embryo yield in lactating and non-lactating llamas. Anim Reprod Sci 119: 322-328. http://dx.doi.org/10.1016/j.anireprosci.2010.02.001

Bourke DA, Adam C.L, Kyle CE, Young P, McEvoy TG, 1992. Superovulation and embryo transfer in the llama. Proc First Int Camel Conf; Allen WR, Higgins AJ, Maythew IG, Snow D, Wade JF (Eds). R&W Publ., Newmarket, UK. pp: 129-137.

Bourke DA, Kyle CE, McEvoy TG, Young P, Adam CL, 1995. Superovulatory responses to eCG in llamas (Lama glama). Theriogenology 44: 255-268. http://dx.doi.org/10.1016/0093-691X(95)00175-8

Bravo PW, Alarcón V, Baca L, Cuba Y, Ordoñez C, Salinas J, Tito F, 2013. Semen preservation and artificial insemination in domesticated South American camelids. Anim Reprod Sci 136: 157-163. http://dx.doi.org/10.1016/j.anireprosci.2012.10.005

Carretero MI, Miragaya M, Chaves MG, Gambarotta M, Agüero A, 2010. Embryo production in superstimulated llamas pre-treated to inhibit follicular growth. Small Rumin Res 88: 32-37. http://dx.doi.org/10.1016/j.smallrumres.2009.11.006

Cerri RI, Chebel RC, Rivera F, Narciso CD, Oliveira RA, Thatcher WW, 2011. Concentration of progesterone during the development of the ovulatory follicle: I. Ovarian and embryonic responses. J Dairy Sci 94: 3342-3351. http://dx.doi.org/10.3168/jds.2010-3734

Chaves MG, Aba M, Agüero A, Egey J, Berestin V, Rutter B, 2002. Ovarian follicular wave pattern and the effect of exogenous progesterone on follicular activity in non-mated llamas. Anim Reprod Sci 69: 37-46. http://dx.doi.org/10.1016/S0378-4320(01)00173-7

Correa JE, Ratto MH, Gatica R, 1997. Superovulation in llamas (Lama glama) with pFSH and equine chorionic gonadotrophin used individually or in combination. Anim Reprod Sci 46: 289-296. http://dx.doi.org/10.1016/S0378-4320(96)01615-6

Fair T, Lonergan P, 2012. The role of progesterone in oocyte acquisition of developmental competence. Reprod Dom Anim 47 (Suppl. 4): 142-147. http://dx.doi.org/10.1111/j.1439-0531.2012.02068.x

Fernandez-Baca S, Hansel, W, Novoa C, 1970. Corpus luteum function in the alpaca. Biol Reprod 3: 252-261.

Huanca W, Cordero A, Huanca T, Cardenas O, Adams GP, Ratto MH, 2009. Ovarian response and embryo production in llamas treated with equine chorionic gonadotropin alone or with a progestin-releasing vaginal sponge at the time of follicular wave emergence. Theriogenology 72: 803-808. http://dx.doi.org/10.1016/j.theriogenology.2009.05.019

IETS, 1998. Manual IETS; Stringfellow DA & Seidel SM (Eds), 3rd Edition. Savoy, IL, USA.

Kanitz W, Becker F, Schneider F, Kanitz E, Leiding C, Nohner H, Pöhland R, 2002. Superovulation in cattle: practical aspects of gonadotropin treatment and insemination. Reprod Nutr Dev 42: 587-599. http://dx.doi.org/10.1051/rnd:2002045

Lonergan P, 2011. Influence of progesterone on oocyte quality and embryo development in cows. Theriogenology 76: 1594-1601. http://dx.doi.org/10.1016/j.theriogenology.2011.06.012

Mapletoft RJ, Bennett Steward K, Adams GP, 2002. Recent advances in the superovulation in cattle. Reprod Nutr Dev 42: 601-611. http://dx.doi.org/10.1051/rnd:2002046

Novoa C, 1981. La conservación de especies nativas en América Latina. Animal Genetic Resources Conservation and Management, FAO Anim Prod Health, Paper 24, FAO, Rome, pp: 349-363.

Rathbone MJ, Macmillan KL, Inskeep K, Burggraaf S, Bunt CR, 1998. Fertility regulation in cattle. J Control Release 54: 117-148. http://dx.doi.org/10.1016/S0168-3659(98)00003-0

Rathbone MJ, Bunt CR, Ogle CR, Burggraaf S, Macmillan KL, Burke CR, Pickering KL. 2002. Reengineering of the commercially available bovine intravaginal insert (CIDR insert) containing progesterone. J Control Release 85: 105-115. http://dx.doi.org/10.1016/S0168-3659(02)00288-2

Ratto MH, Gatica R, Correa JE, 1997. Timing of mating and ovarian response in llamas (Lama glama) treated with pFSH. Anim Reprod Sci 48: 325-330. http://dx.doi.org/10.1016/S0378-4320(97)00047-X

Richards MW, Spitzer JC, Warner MB, 1986. Effect of varying levels of postpartum nutrition at body condition at calving on subsequent reproductive performance in beef cattle. J Anim Sci 62: 300-306.

Rivera FA, Mendonca LG, Lopes GJr, Santos JEP, Perez RV, Amstalden M, Correa-Calderón A, Chebel RC, 2011. Reduced progesterone concentration during growth of the first follicular wave affects embryo quality but has no effect on embryo survival post transfer in lactating dairy cows. Reproduction 141: 333-342. http://dx.doi.org/10.1530/REP-10-0375

Salhab M, Tosca L, Cabau C, Papillier P, Perreau C, Dupont J, Mermillod P, Uzbekova S, 2011. Kinetics of gene expression and signaling in bovine cumulus cells throughout IVM in different mediums in relation to oocyte developmental competence, cumulus apoptosis and P4 secretion. Theriogenology 75: 90-104. http://dx.doi.org/10.1016/j.theriogenology.2010.07.014

San-Martin M, Copaira M, Zuniga J, Rodriguez R, Bustinza G, Acosta L, 1968. Aspects of reproduction in the alpaca. J Reprod Fertil 16: 395-399. http://dx.doi.org/10.1530/jrf.0.0160395

SAS, 1989. SAS/STAT® User’s Guide, Version 6, vol. 2, 4th ed. SAS Institute Inc., Cary, NC, USA. 848 pp.

Steel RGD, Torrie JH, 1980. Principles and procedures of statistics, 2nd ed. McGraw-Hill, NY, USA.

van Werven T, Waldeck F, Souza AH, Floch S, Englebienne M, 2013. Comparison of two intravaginal progesterone releasing devices (PRID-Delta vs CIDR) in dairy cows: Blood progesterone profile and field fertility. Anim Reprod Sci 138: 143-149. http://dx.doi.org/10.1016/j.anireprosci.2013.02.010

Vaughan J, Mihm M, Wittek T, 2013. Factors influencing embryo transfer success in alpacas-A retrospective study. Anim Reprod Sci 136: 194-204. http://dx.doi.org/10.1016/j.anireprosci.2012.10.010




DOI: 10.5424/sjar/2015133-7308