Short communication: Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the cecal tonsil of broiler chickens

Chunyu Liu, Min Jiang, Jing Fang, Xi Peng, Hengmin Cui

Abstract


Aflatoxin B1 (AFB1) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB1 on the cytokines expression and T-cell subsets in the cecal tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB1/kg feed) and AFB1 group (0.6 mg pure AFB1/kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the cecal tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the cecal tonsils of broiler chickens, suggesting that the cell-mediated immunity of cecal tonsils might be impaired in broilers.


Keywords


aflatoxin B1; cell-mediated immunity; cecal tonsil; broiler chicken

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References


Chen K, Yuan S, Chen J, Peng X, Wang F, Cui H, Fang J, 2013a. Effects of sodium selenite on the decreased percentage of T cell subsets, contents of serum IL-2 and IFN-γ induced by aflatoxin B1in broilers. Res Vet Sci 95 (1): 143-145. http://dx.doi.org/10.1016/j.rvsc.2013.02.019

Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, 2013b. Protective role of sodium selenite on histopathological lesions, decreased T-cell subsets and increased apoptosis of thymus in broilers intoxicated with aflatoxin B1. Food Chem Toxicol 59: 446-454. http://dx.doi.org/10.1016/j.fct.2013.06.032

Chen K, Peng X, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W, Tang L, 2014. Effects of dietary selenium on histopathological changes and T cells of spleen in broilers exposed to aflatoxin B1. Int J Env Res Public Health 11 (2): 1904-1913. http://dx.doi.org/10.3390/ijerph110201904

Deng Y, Cui H, Peng X, Fang J, Zuo Z, Wang K, Cui W, Wu B, 2012. Changes of IgA+ cells and cytokines in the cecal tonsil of broilers fed on diets supplemented with vanadium. Biol Trace Elem Res 147 (1-3): 149-155. http://dx.doi.org/10.1007/s12011-012-9330-z

Erf GF, Bottje WG, Bersi TK, 1998. CD4, CD8 and TCR defined T-cell subsets in thymus and spleen of 2-and 7-week old commercial broiler chickens. Vet Immunol Immunopathol 62 (4): 339-348. http://dx.doi.org/10.1016/S0165-2427(97)00070-6

Guo S, Liao S, Su R, Lin R, Chen Y, Tang Z, Wu H, Shi D, 2012. Influence of Longdan Xiegan decoction on body weights and immune organ indexes in ducklings intoxicated with aflatoxin B1. J Anim Vet 11: 1162-1165. http://dx.doi.org/10.3923/javaa.2012.1162.1165

Hatori Y, Sharma RP, Warren RP, 1991. Resistance of C57B1/6 mice to immunosuppressive effects of aflatoxin B1 and relationship with neuroendocrine mechanisms. Immunopharmacology 22 (2): 127-136. http://dx.doi.org/10.1016/0162-3109(91)90037-Y

Hinton DM, Myers MJ, Raybourne RA, Francke-Carroll S, Sotomayor RE, Shaddock J, Warbritton A, Chou MW, 2003. Immunotoxicity of aflatoxin B1 in rats: effects on lymphocytes and the inflammatory response in a chronic intermittent dosing study. Toxicol Sci 73 (2): 362-377. http://dx.doi.org/10.1093/toxsci/kfg074

Huang J, Cui H, Peng X, Fang J, Zuo Z, Deng J, Wang X, Wu B, 2014. Effect of dietary nickel chloride on splenic immune function in broilers. Biol Trace Elem Res 159 (1-3): 183-191. http://dx.doi.org/10.1007/s12011-014-0003-y

Humberd J, Boyd K, Webster RG, 2007. Emergence of influenza A virus variants after prolonged shedding from pheasants. J Virol 81 (8): 4044-4051. http://dx.doi.org/10.1128/JVI.02346-06

Kantrow S, Meyer K, Kidd P, Raghu G, 1997. The CD4/CD8 ratio in BAL fluid is highly variable in sarcoidosis. Eur Respir J 10 (12): 2716-2721. http://dx.doi.org/10.1183/09031936.97.10122716

Kidd M, Ferket P, Qureshi M, 1996. Zinc metabolism with special reference to its role in immunity. Worlds Poult Sci 52 (3): 309-324. http://dx.doi.org/10.1079/WPS19960022

Li Y, Ma QG, Zhao LH, Wei H, Duan GX, Zhang JY, Ji C, 2014. Effects of lipoic acid on immune function, the antioxidant defense system, and inflammation-related genes expression of broiler chickens fed aflatoxin contaminated diets. Int J Mol Sci 15 (4): 5649-5662. http://dx.doi.org/10.3390/ijms15045649

Liu J, Cui H, Peng X, Fang J, Zuo Z, Wang H, Wu B, Deng Y, Wang K, 2012. Decreased percentages of T-cell subsets and IL-2 contents in the cecal tonsil of broilers fed diets high in fluorine. Fluoride 45 (1): 53-57.

Meissonnier GM, Pinton P, Laffitte J, Cossalter AM, Gong YY, Wild CP, Bertin G, Galtier P, Oswald IP, 2008. Immunotoxicity of aflatoxin B1: impairment of the cell-mediated response to vaccine antigen and modulation of cytokine expression. Toxicol Appl Pharmacol 231 (2): 142-149. http://dx.doi.org/10.1016/j.taap.2008.04.004

Mohamed AM, Metwally NS, 2009. Antiaflatoxigenic activities of some plant aqueous extracts against aflatoxin-B1 induced renal and cardiac damage. J Pha Tox 4 (1): 1-16. http://dx.doi.org/10.3923/jpt.2009.1.16

Olah I, Glick B, Taylor R, 1984. Meckel's diverticulum. II. A novel lymphoepithelial organ in the chicken. Anat Rec 208 (2): 253-263. http://dx.doi.org/10.1002/ar.1092080212

Qian G, Tang L, Guo X, Wang F, Massey ME, Su J, Guo TL, Williams JH, Phillips TD, Wang JS, 2014. Aflatoxin B1 modulates the expression of phenotypic markers and cytokines by splenic lymphocytes of male F344 rats. J Appl Toxicol 34 (3): 241-249. http://dx.doi.org/10.1002/jat.2866

Scott P, 1993. IL-12: initiation cytokine for cell-mediated immunity. Science 260: 496-497. http://dx.doi.org/10.1126/science.8097337

Shini S, Kaiser P, 2009. Effects of stress, mimicked by administration of corticosterone in drinking water, on the expression of chicken cytokine and chemokine genes in lymphocytes. Stress 12 (5): 388-399. http://dx.doi.org/10.1080/10253890802526894

Tomková I, Ševčíková Z, Levkut M, Revajová V, Čonková E, Laciaková A, Lenhardt L, 2002. Effect of aflatoxin B1 on CD3 T cells and alkaline phosphatase in the intestine of mice. Mycopathologia 154 (1): 15-19. http://dx.doi.org/10.1023/A:1015296523901




DOI: 10.5424/sjar/2016143-8811