Effect of space allowance during transport and fasting or non-fasting during lairage on carcass contamination and meat traits in Merino lamb

  • Herminia Vergara Universidad de Castilla-La Mancha, Campus Universitario, ETS de Ingenieros Agrónomos y de Montes, Dept. Ciencia y Tecnología Agroforestal y Genética. 02071 Albacete
  • Almudena Cózar Universidad de Castilla-La Mancha, Campus Universitario, ETS de Ingenieros Agrónomos y de Montes, Dept. Ciencia y Tecnología Agroforestal y Genética. 02071 Albacete
  • Ana I. Rodríguez NCARLOPSA, Tarancón (Cuenca)
  • Luis Calvo NCARLOPSA, Tarancón (Cuenca)
Keywords: sheep, transportation, pre-slaughter conditions, carcass-contamination, meat quality

Abstract

A total of 72 Merino breed male lambs were used in this work, to study the effect of the space allowance during transport [(SA): low (SAL: 0.16 m2/animal; n=24); medium (SAM: 20 m2/animal; n=24); high (SAH: 0.30 m2/animal; n=24)], and the management during 18 h lairage [(TL): fasting (TL-FAST; n=36) vs feeding (TL-FEED; n=36)] on carcass microbial contamination (total viable count, Enterobacteriaceae and Pseudomonas) and meat quality. Carcasses contamination determination was carried out by swabbing (neck, flank and rump). Meat quality was assessed by pH, colour coordinates, drip loss (DL), shear force (SF) ad lipid oxidation. SA did not have effect on carcass microbiological quality. TL caused a significant effect on total viable count and Pseudomonas spp values. Flank was the most contaminated site. SAL-FEED group showed the highest values of drip loss and lipid oxidation. At 24 h post-mortem, pH values were the highest in fasted lambs. At 7 d post-mortem the lowest pH was found in SAM-FAST group while the highest in SAM-FEED. TL had no effect on SF, DL neither on lipid oxidation values. These results could help to meat industry to decide the best management as in the transportation as during lairage before lambs slaughter.

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References

Abril M, Campo MM, Önenç A, Sa-udo C, Albertí P, Negueruela AI, 2001. Beef colour evolution as a function of ultimate pH. Meat Sci 58 (1): 69-78. https://doi.org/10.1016/S0309-1740(00)00133-9

Adzitey F, 2011. Effect of pre-slaughter animal handling on carcass and meat quality. Int Food Res J 18 (2): 485-491.

Bell RG, 1997. Distribution and sources of microbial contamination on beef carcasses. J Appl Microbiol 82 (3): 292-300. https://doi.org/10.1046/j.1365-2672.1997.00356.x

Biss ME, Hathaway SC, 1996. Microbiological contamination of ovine carcasses associated with the presence of wool and faecal material. J Appl Bacteriol 81 (6): 594-600. https://doi.org/10.1111/j.1365-2672.1996.tb03552.x

Cózar A, Rodríguez AI, Garijo P, Calvo L, Vergara H, 2016. Effect of space allowance during transport and fasting or non-fasting during lairage on welfare indicators in Merino lambs. Span J Agric Res 14 (1): e0501. https://doi.org/10.5424/sjar/2016141-8313

Chulayo AY, Muchenje V, 2013. The effects of pre-slaughter stress and season on the activity of plasma creatine kinase and mutton quality from different sheep breeds slaughtered at a smallholder abattoir. Asian-Australas J Anim Sci 26 (12): 1762-1772. https://doi.org/10.5713/ajas.2013.13141

De la Fuente J, Sánchez M, Pérez C, Lauzurica S, Vieira C, González de Chavarri E, Díaz MT, 2010. Physiological response and carcass and meat quality of suckling lambs in relation to transport time and stocking density during transport by road. Animal 4 (2): 250-258. https://doi.org/10.1017/S1751731109991108

De la Fuente J, González de Chávarri E, Sánchez M, Vieira C, Lauzurica S, Diaz MT, Pérez C, 2012. The effects of journey duration and space allowance on the behavioural and biochemical measurements of stress responses in suckling lambs during transport to an abattoir. Appl Anim Behav Sci 142 (1): 30-41. https://doi.org/10.1016/j.applanim.2012.08.010

Díaz MT, Vieira C, Pérez C, Lauzurica S, González de Chávarri E, Sánchez M, De la Fuente J, 2014. Effect of lairage time (0 h, 3 h, 6 h or 12 h) on glycogen content and meat quality parameters in suckling lambs. Meat Sci 96 (2): 653-660. https://doi.org/10.1016/j.meatsci.2013.10.013

Duncan IJH, 2004. Pain, fear and distress. In: Applying science to animal welfare. Proc of Global Conf on Animal Welfare: an OIE initiative. pp: 163-171. Paris, 23-25 Feb.

EC, 2001. Commission Decision (2001/471/EC) of 8 June 2001 laying down rules for the regular checks on the general hygiene carried out by the operators in establishments according to Directive 64/433/EEC on health conditions for the production and marketing of fresh meat and Directive 71/118/EEC on health problems affecting the production and placing on the market of fresh poultry meat. Official Journal L 165, 21/06/2001 P. 0048-0053.

Ekiz B, Ekiz EE, Kocak O, Yalcintan H, Yilmaz A, 2012. Effect of pre-slaughter management regarding transportation and time in lairage on certain stress parameters, carcass and meat quality characteristics in Kivircik lambs. Meat Sci 90 (4): 967-976. https://doi.org/10.1016/j.meatsci.2011.11.042

Ferguson DM, Warner RD, 2008. Have we underestimated the impact of pre-slaughter stress on meat quality in ruminants? Meat Sci 80: 12-19. https://doi.org/10.1016/j.meatsci.2008.05.004

Fisher MW, Muir PD, Gregory NG, 2011. The animal welfare implications of depriving sheep of feed to facilitate transport and slaughter. MAF Technical, 37.

French P, O'Riordan EG, Monahan FJ, Caffrey PJ, Vidal M, Mooney MT, Troy DJ, Moloney AP, 2000. Meat quality of steers finished on autumn grass, grass silage or concentrate-based diets. Meat Sci 56 (2): 173-180. https://doi.org/10.1016/S0309-1740(00)00037-1

Grandin T, 1980. The effect of stress on livestock and meat quality prior to and during slaughter. Int J Stud Anim Problem 1 (5): 313-337.

Grandin T, 2007. Introduction: Effect of customer requirements, international standards and marketing structure on the handling and transport of livestock and poultry. In: Livestock handling and transport; Grandin T (ed.), 3rd Edn. pp: 1-18. CABI Publ, Wallingford, UK. https://doi.org/10.1079/9781845932190.0001

Grau FH, Smith MG, 1974. Salmonella contamination of sheep and mutton carcasses related to pre‐slaughter holding conditions J Appl Bacteriol 37 (1): 111-116. https://doi.org/10.1111/j.1365-2672.1974.tb00421.x

Gregory NG, Grandin T, 1998. Animal welfare and meat science. CABI Publ, Wallingford, UK. 298 pp.

Hall SJ, Bradshaw RH, 1998. Welfare aspects of the transport by road of sheep and pigs. J Appl Anim Welf Sci 1 (3): 235-254. https://doi.org/10.1207/s15327604jaws0103_4

Huff-Lonergan E, Lonergan SM, 2005. Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes. Meat Sci 71(1): 194-204. https://doi.org/10.1016/j.meatsci.2005.04.022

Kannan G, Terrill TH, Kouakou B, Gazal OS, Gelaye S, Amoah EA, Samaké S, 2000. Transportation of goats: Effects on physiological stress responses and live weight loss. J Anim Sci 78 (6): 1450-1457. https://doi.org/10.2527/2000.7861450x

Kannan G, Terrill TH, Kouakou B, Gelaye S, Amoah EA, 2002. Simulated pre-slaughter holding and isolation effects on stress responses and live weight shrinkage in meat goats. J Anim Sci 80 (7): 1771-1780. https://doi.org/10.2527/2002.8071771x

Knowless TG, 1998. A review of the road transport of slaughter sheep. Vet Record 143: 212-219. https://doi.org/10.1136/vr.143.8.212

Lawrie RA, 1998. Lawrie's meat science. 6th Edn. Woodhead Publ Ltd, Cambridge, UK. pp: 1-288.

Liste G, Miranda-de la Lama GC, Campo MM, Villarroel M, Muela E, María GA, 2011. Effect of lairage on lamb welfare and meat quality. Anim Prod Sci 51: 952-958. https://doi.org/10.1071/AN10274

Miranda-de la Lama GC, Liste G, Villarroel M, Escós J, María GA, 2010. Critical points in the pre-slaughter logistic chain of lambs in Spain that may compromise the animal's welfare. Small Rumin Res 90 (1): 174-178. https://doi.org/10.1016/j.smallrumres.2010.02.011

Miranda-de la Lama GC, 2013. Transport and pre-slaughter logistics: definitions and current tendencies in animal welfare and meat quality. Veterinaria México 44 (1): 31-56.

Nikbin S, Panandam JM, Awis Q, Sazili AQ, 2016. Influence of pre-slaughter transportation and stocking density on carcass and meat quality characteristics of Boer goats, Italian J Anim Sci 15 (3): 504-511. https://doi.org/10.1080/1828051X.2016.1217752

Pointon A, Kiermeier A, Fegan N, 2012. Review of the impact of pre-slaughter feed curfews of cattle, sheep and goats on food safety and carcase hygiene in Australia. Food Control 26 (2): 313-321. https://doi.org/10.1016/j.foodcont.2012.01.034

Rabaste C, Faucitano L, Saucier L, Mormède P, Correa JA, Giguère A, Bergeron R, 2007. The effects of handling and group size on welfare of pigs in lairage and their influence on stomach weight, carcass microbial contamination and meat quality. Can J Anim Sci 87(1): 3-12. https://doi.org/10.4141/A06-041

Sánchez-Sánchez M, Vieira-Aller C, De-La-Fuente-Vázquez J, Perez-Marcos C, Lauzurica-Gómez S, González-De-Chavarri E, Díaz-Díaz-Chiron MT, 2013. Effect of season and stocking density during transport on carcass and meat quality of suckling lambs. Span J Agric Res 11 (2): 394-404. https://doi.org/10.5424/sjar/2013112-3274

Sa-udo C, Sánchez A, Alfonso M, 1998. Small ruminant production systems and factors affecting lamb meat quality. Meat Sci 49: S29-S64. https://doi.org/10.1016/S0309-1740(98)90037-7

Sävendahl L, Underwood LE, 1999. Fasting increases serum total cholesterol, LDL cholesterol and apolipoprotein B in healthy, nonobese humans. J Nutr 129 (11): 2005-2008.

Tarladgis BG, Pearson AM, Dugan R, 1964. Chemistry of the 2-thiobarbituric test for determination of oxidative rancidity in foods II. Formation of the TBA-malonaldehyde complex without acid-heat treatment. J Sci Food Agric 5: 602-604. https://doi.org/10.1002/jsfa.2740150904

Tejeda JF, Pe-a RE, Andrés AI, 2008. Effect of live weight and sex on physico-chemical and sensorial characteristics of Merino lamb meat. Meat Sci 80 (4): 1061-1067. https://doi.org/10.1016/j.meatsci.2008.04.026

Teke B, Ekiz B, Akdag F, Ugurlu M, Ciftci G, Senturk B, 2014. Effects of stocking density of lambs on biochemical stress parameters and meat quality related to commercial transportation. Ann Anim Sci 14 (3): 611-621. https://doi.org/10.2478/aoas-2014-0012

Terlouw C, 2005. Stress reactions at slaughter and meat quality in pigs: genetic background and prior experience-A brief review of recent findings. Liv Prod Sci 94: 125-135. https://doi.org/10.1016/j.livprodsci.2004.11.032

Vanguru M, Lee JH, Kouakou B, Terrill TH, Kannan G, 2009. Effect of feed deprivation time on bacterial contamination of skin and carcass in meat goats. Trop Subtrop Agroecos 11 (1): 259-261.

Vergara H, Molina A, Gallego L, 1999. Influence of sex and slaughter weight on carcass and meat quality in light and medium weight lambs produced in intensive systems. Meat Sci 52: 221-226. https://doi.org/10.1016/S0309-1740(98)00171-5

Vergara H, Gallego L, García A, Landete-Castillejos T, 2003. Conservation of Cervus elaphus meat in modified atmospheres. Meat Sci 65: 779-783. https://doi.org/10.1016/S0309-1740(02)00282-6

Warris PD, 1985. Marketing losses caused by fasting and transport during the pre-slaughter handling of pigs. Pig News and Information 6: 155-157.

Warriss PD, 2003. Optimal lairage times and conditions for slaughter pigs: a review. Vet Rec 153 (6): 170-176. https://doi.org/10.1136/vr.153.6.170

Zhong RZ, Liu HW, Zhou DW, Sun HX, Zhao CS, 2011. The effects of road transportation on physiological responses and meat quality in sheep differing in age. J Anim Sci 89 (11): 3742-3751. https://doi.org/10.2527/jas.2010-3693

Zimerman M, Domingo E, Grigioni G, Taddeo H, Willems P, 2013. The effect of pre-slaughter stressors on physiological indicators and meat quality traits on Merino lambs. Small Rumin Res 111 (1): 6-9. https://doi.org/10.1016/j.smallrumres.2012.12.018

Zweifel Z, Stephan R, 2003. Microbiological monitoring of sheep carcass contamination in three Swiss abattoirs. J Food Prot 66 (6): 946-952. https://doi.org/10.4315/0362-028X-66.6.946

Published
2017-07-31
How to Cite
Vergara, H., Cózar, A., Rodríguez, A. I., & Calvo, L. (2017). Effect of space allowance during transport and fasting or non-fasting during lairage on carcass contamination and meat traits in Merino lamb. Spanish Journal of Agricultural Research, 15(2), e0503. https://doi.org/10.5424/sjar/2017152-10227
Section
Animal health and welfare