Short communication: Morphological description and molecular detection of Pestalotiopsis sp. on hazelnut in Serbia

  • Tanja Vasić Institute for Forage Crops, 37251 Kruševac
  • Darko Jevremović Fruit Research Institute, Kralja Petra I 9, 32000 Čačak http://orcid.org/0000-0002-2983-9198
  • Vesna Krnjaja Institute for Animal Husbandry, Autoput 16, 11080 Belgrade
  • Aleksandar Leposavić Fruit Research Institute, Kralja Petra I 9, 32000 Čačak
  • Snežana Anđelković Institute for Forage Crops, 37251 Kruševac
  • Sanja Živković University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade
  • Svetlana Paunović Fruit Research Institute, Kralja Petra I 9, 32000 Čačak
Keywords: Corylus avellana L., leaf blight, β-tubulin, ITS, EF1-α

Abstract

In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch’s postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-α, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.

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Published
2017-12-21
How to Cite
Vasić, T., Jevremović, D., Krnjaja, V., Leposavić, A., Anđelković, S., Živković, S., & Paunović, S. (2017). Short communication: Morphological description and molecular detection of Pestalotiopsis sp. on hazelnut in Serbia. Spanish Journal of Agricultural Research, 15(3), e10SC02. https://doi.org/10.5424/sjar/2017153-11297
Section
Plant protection

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