Valine in diets for juvenile Nile tilapia (Oreochromis niloticus): growth performance, chemical composition, blood parameters and skeletal muscle development

  • Rômulo B. Rodrigues Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, Bairro Agronomia 7712, 91540-000 Porto Alegre-RS
  • Micheli Z. Hassemer Universidade Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza 200, Centro Cívico, 87030-900 Mogi das Cruzes-SP
  • Iury W. A. Melo Universidade Estadual do Oeste do Paraná, Centro de Engenharias e Ciências Exatas. Rua da Faculdade 645, 85903-000 Toledo-PR
  • Dacley H. Neu Universidade Federal da Grande Dourados, Rod. Dourados a Itahum km 12, 79804-970 Dourados-MS
  • Fábio Bittencourt Universidade Estadual do Oeste do Paraná, Centro de Engenharias e Ciências Exatas. Rua da Faculdade 645, 85903-000 Toledo-PR
  • Wilson R. Boscolo Universidade Estadual do Oeste do Paraná, Centro de Engenharias e Ciências Exatas. Rua da Faculdade 645, 85903-000 Toledo-PR
Keywords: animal health, aquaculture, branched-chain amino acids, essential amino acid, fish nutrition, hematology, histology

Abstract

Valine belongs to the group of branched-chain amino acids, has an important structural role and is primarily deposited as body protein. The present study evaluated the effects of valine in diets of juvenile Nile tilapia. A total of 216 juveniles with weight of 21.40 ± 0.42 g and length of 10.07 ± 1.00 cm were distributed into 18 aquarium in a completely randomized design with six treatments and three replicates. Six diets containing 24.30% digestible protein, 3,100 kcal/kg digestible energy, and increasing valine levels (0.54; 0.63; 0.72; 0.81; 0.90; 0.99%) were prepared. The following factors were evaluated: performance, chemical composition, blood hematological and biochemical, and skeletal muscle development. Differences were observed in productive performance between treatments for weight gain, daily weight gain and apparent feed conversion, where inclusion levels from 0.81% to 0.99% provide better performances. According to the equation of quadratic regression, the inclusion of 0.86% of valine provided greater weight gain. Regarding the hematological and biochemical parameters, there were differences among the treatments for hemoglobin, triglycerides, and cholesterol. Not difference was observed for the chemical composition and muscle fiber growth. It is recommended the inclusion of 0.86% of valine in the diet of juveniles of Nile tilapia because it provides greater weight gain.

Downloads

Download data is not yet available.

References

Abidi SF, Khan MA, 2007. Dietary leucine requirement of fingerling Indian major carp, Labeo rohita (Hamilton). Aquac Res 38: 478-486. https://doi.org/10.1111/j.1365-2109.2007.01687.x

Almeida FLA, Carvalho RF, Pinhal D, Padovani CR, Martins C, Dal Pai-Silva M, 2008. Differential expression of myogenic regulatory factor MyoD in pacu skeletal muscle (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei) during juvenile and adult growth phases. Micron 39: 1306-1311. https://doi.org/10.1016/j.micron.2008.02.011

AOAC, 2005. Official methods of analysis of association of official analytical chemists. Official Analytical Chemists International, 18th ed. Washington, DC, USA.

Calder PC, 2006. Branched-chain amino acids and immunity. J Nutr 136: 288-293. https://doi.org/10.1093/jn/136.1.288S

Chen CY, Wooster GA, Getchell RG, Bowser PR, Timmons MB, 2003. Blood chemistry of healthy, nephrocalcinosis‑affected and ozone‑treated tilapia in a recirculation system, with application of discriminant analysis. Aquaculture 218: 89‑102. https://doi.org/10.1016/S0044-8486(02)00499-4

Collier HB, 1944. The standardization of blood haemoglobin determinations. Can Med Assoc J 50: 550-552.

Deriggi GF, Inoue LAKA, Moraes G, 2006. Stress responses to handling in Nile tilapia (Oreochromis niloticus Linnaeus): assessment of eugenol as an alternative anesthetic. Acta Sci Biol Sci 28: 269-274.

Dong M, Feng L, Kuang SY, Liu Y, Jiang J, Hu K, Jiang WD, Li SH, Tang L, Zhou XQ, 2012. Growth, body composition, intestinal enzyme activities and microflora of juvenile Jian carp (Cyprinus carpio var. Jian) fed graded levels of dietary valine. Aquac Nutr 19: 1-14. https://doi.org/10.1111/j.1365-2095.2011.00926.x

Feng L, Luo JB, Jiang WD, Liu Y, Wu P, Jiang J, Kuang SY, Tang L, Zhang YA, Zhou XQ, 2015. Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression. Fish Shellfish Imunn 45: 239-249. https://doi.org/10.1016/j.fsi.2015.04.023

Fernstrom JD, 2005. 4th amino acid assessment workshop: Branched chain amino acids and brain function. J Nutr 135: 1539-1546. https://doi.org/10.1093/jn/135.6.1539S

Furuya WM, 2010. Tabelas brasileiras para a nutrição de tilápias. GFM, Toledo-PR, Brazil. 101 pp.

Goldenfarb PB, Bowyer FP, Hall E, Brosius E, 1971. Reproducibility in the hematology laboratory: the microhematocrit determinations. Am J Clin Path 56: 35-39. https://doi.org/10.1093/ajcp/56.1.35

Han Y, Han R, Koshio S, Ishikawa M, Yokoyama S, Gao J, 2014. Interactive effects of dietary valine and leucine on two sizes of Japanese flounder Paralichthys olivaceus. Aquaculture 432: 130-138. https://doi.org/10.1016/j.aquaculture.2014.05.004

Harper AE, Miller RH, Block KP, 1984. Branched-chain amino acid metabolism. Annu Ver Nutr 4: 409-454. https://doi.org/10.1146/annurev.nu.04.070184.002205

Hrubec TC, Cardinale JL, Smith SA, 2000. Haematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Vet Clin Pathol 29: 7-12. https://doi.org/10.1111/j.1939-165X.2000.tb00389.x

Hughes SG, Rumsey LJ, Nesheim MC, 1984. Effects of dietary excesses of branched-chain amino acids on the metabolism and tissue composition of lake trout (Salvelinus namaycush). Comp Biochem Physio l A Comp Physiol 78: 413-418. https://doi.org/10.1016/0300-9629(84)90569-3

Koumans JTM, Akster HA, 1995. Myogenic cells in development and growth of fish. Comp Biochem Physio 110: 3-20. https://doi.org/10.1016/0300-9629(94)00150-R

Li P, Mai KS, Trushenski J, Wu GY, 2009. New developments in fish amino acid nutrition: towards functional and environmentally oriented aquafeeds. Amino Acids 37: 43-53. https://doi.org/10.1007/s00726-008-0171-1

NRC, 2011. Nutrient requirements of fish and shrimp. National Research Council, National Academy Press, Washington, DC. 390 pp.

Rahimnejad S, Lee KJ, 2013. Dietary valine requirement of juvenile red sea bream Pagrus major. Aquaculture 416-417: 212-218. https://doi.org/10.1016/j.aquaculture.2013.09.026

Ranzani-Paiva MJT, Benites de Pádua S, Tavares-Dias M, Egami MI, 2013. Métodos para análise hematológica em peixes. Eduem, Maringá-PR, Brazil. 140 pp. https://doi.org/10.7476/9788576286530

Rosenfeld G, 1947. Corante pancrômico para hematologia e citologia clínica. Nova combinação dos componentes do May-Grünwald e do Giemsa num só corante de emprego rápido. Mem Inst Butantan 20: 329-334.

Santiago CB, Lovell RT, 1988. Amino acid requirement for growth of Nile tilapia. J Nutr 118: 1540-1546. https://doi.org/10.1093/jn/118.12.1540

Shimomura Y, Yamamoto Y, Bajotto GS, 2006. Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr 36: 529S-532S. https://doi.org/10.1093/jn/136.2.529S

Suryawan A, Orellana RA, Fiorotto ML, Davis TA, 2011. Leucine acts as a nutrient signal to stimulate protein synthesis in neonatal pigs. J Anim Sci 89: 2004-2016. https://doi.org/10.2527/jas.2010-3400

Tavares-Dias M, Moraes FR, 2004. Hematologia de peixes teleósteos. Editora Eletrônica e Arte Final, Ribeirão Preto-SP, Brazil. 144 pp.

Valente LMP, Rocha E, Gomes EFS, Silva MW, Oliveira MH, Monteiro RAF, Fauconneau B, 1999. Growth and dynamics of white and red muscle fibres in fast- and slow-growing strains of rainbow trout. J Fish Biol 55: 675-691. https://doi.org/10.1111/j.1095-8649.1999.tb00710.x

Wilson RP, 1985. Amino acid and protein requirements of fish. In: Nutrition and feeding fish; Cowey CB, Mackie AM, Bell JG (eds.). pp: 1-16. Acad Press, London.

Wilson RP, 2002. Amino acids and proteins. In: Fish Nutrition; Halver JE, Hardy RW (eds.). pp: 143-179. Acad Press, NY. https://doi.org/10.1016/B978-012319652-1/50004-5

Wu G, 2009. Amino acids: metabolism, functions, and nutrition. Amino Acids 37: 1-17. https://doi.org/10.1007/s00726-009-0269-0

Published
2019-07-26
How to Cite
Rodrigues, R. B., Hassemer, M. Z., Melo, I. W. A., Neu, D. H., Bittencourt, F., & Boscolo, W. R. (2019). Valine in diets for juvenile Nile tilapia (Oreochromis niloticus): growth performance, chemical composition, blood parameters and skeletal muscle development. Spanish Journal of Agricultural Research, 17(2), e0602. https://doi.org/10.5424/sjar/2019172-13953
Section
Animal production