Male sterility of triticale lines generated through recombination of triticale and rye maintainers

  • Tomasz Warzecha University of Agriculture in Krakow, Dept. Plant Breeding and Seed Science. Łobzowska str. No. 24, 31-140 Kraków
  • Agnieszka Sutkowska University of Agriculture in Krakow, Dept. Plant Breeding and Seed Science. Łobzowska str. No. 24, 31-140 Kraków
  • Halina Góral University of Agriculture in Krakow, Dept. Plant Breeding and Seed Science. Łobzowska str. No. 24, 31-140 Kraków
Keywords: cms systems, cytoplasm, Ttriticum timopheevi, Pampa, ISSR

Abstract

The Triticum timopheevi cytoplasmic male sterility (cms) system in triticale (xTriticosecale Wittmack) suffers from a low frequency of maintainers and environmental instability of the male sterility. On the other hand, the Pampa cms system in rye (Secale cereale) exhibits strong male sterility and a low frequency of restorers. Here, we report generating hybrids between maintainers of the T. timopheevi cms system in triticale and maintainers of the rye Pampa cms system. Ten hybrids were obtained. Their hybridity was verified by PCR (polymerase chain reaction) using ISSR (inter simple sequence repeats) primers. The cms maintaining ability of F2 individuals and their progeny was tested. The F2 plants were crossed to male sterile lines of triticale carrying the T. timopheevi cytoplasm. Among 180 G1 offspring of these crosses, 71 (39.4%) were completely male sterile. Fourteen F2 individuals (7.8%), as well as their F2S1 and progeny, generated stable male sterility in G1, G1BC1 and G1BC2 generations after the crosses. Our results suggest that it is possible to produce a more stable cms system in triticale based on the T. timopheevi cytoplasm as compared to the existing one.

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References

References

Ammar K, Crossa J, Pfeiffer WH, 2006. Developing a hybrid seed production system and evaluation of heterosis levels in hybrids from CIMMYT's spring triticale germplasm. Proc. of the 6th Int. Triticale Symp. Stellenbosch, South Africa, 3-7 September, pp: 65-67.

Bianco CL, Juan A, Fernández JA, Migliaro D, Crino P, Catalina Egea-Gilabert, 2011. Identification of F1 hybrids of artichoke by ISSR markers and morphological analysis. Mol Breeding 27: 157-170. http://dx.doi.org/10.1007/s11032-010-9418-0

Carvalho A, Matos M, Lima-Brito J, Guedes-Pinto H, Benito C, 2005. DNA fingerprint of F1 interspecific hybrids from Triticeae tribe using ISSRs. Euphytica 143: 93-99. http://dx.doi.org/10.1007/s10681-005-2839-x

Curtis CA, Lukaszewski AJ, 1993. Localization of genes in rye that restore male fertility to hexaploid wheat with T. timopheevi cytoplasm. Plant Breed 111: 106-112. http://dx.doi.org/10.1111/j.1439-0523.1993.tb00615.x

Feldman M, Liu B, Segal G, Abbo S, Levy AA, Vega JM, 1997. Rapid elimination of low- copy DNA sequences in polyploid wheat: A possible mechanism for differentiation of homoeologous chromosomes. Genetics 147: 1381-1387.

Feldman M, Levy AA, Fahima T, Korol A, 2012. Genomic asymmetry in allopolyploid plants: wheat as a model. J Exp Bot 63: 5045-5059. http://dx.doi.org/10.1093/jxb/ers192

Geiger HH, Schnell FW, 1970. Cytoplasmic male sterility in rye (Secale cereale L.). Crop Sci 10: 590-593. http://dx.doi.org/10.2135/cropsci1970.0011183X001000050043x

Góral H, 2002. Production of triticale (× Triticosecale Wittm.) hybrid seeds using the sterilizing cytoplasm of Triticum timopheevi. Cereal Res Com 30: 31-38.

Góral H, Spiss L, 2005. Hodowla dopełniaczy i restorerów dla systemu cms-T. timopheevi u pszenżyta jarego. Bull IHAR 236: 99-104.

Góral H, Warzecha T, Stojałowski S, Pojmaj M, Kurleto D, Trąbka A, Spiss L, 2006. Stability of male sterility and fertility restoration in the cms-T. timopheevi system in triticale. Folia Univ Agric Stetin 247 (100): 55-62.

Góral H, Pojmaj MS, Pojmaj R, 2007. Frekwencja genotypów dopełniających i restorujących dla systemu cms-T. timopheevi u pszenżyta ozimego. Bull IHAR 244: 155-160.

Grądzielewska A, Gruszecka D, Leśniowska-Nowak J, Paczos-Grzęda E, 2012. Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig. and determination of genetic similarity with ISSRs. Genet Mol Res 11(3): 2147-2155. http://dx.doi.org/10.4238/2012.July.10.1

Guedes-Pinto H, Lima-Britto J, Ribeiri-Carvalho C, Gustafson JP, 2001. Genetic control of crossability of triticale and rye. Plant Breeding 120: 27-31. http://dx.doi.org/10.1046/j.1439-0523.2001.00545.x

Han F, Fedak G, Guo W, Bao Liu B, 2005. Rapid and repeatable elimination of a parental genome-specific DNA repeat (pGc1R-1a) in newly synthesized wheat allopolyploids. Genetics 170: 1239-1245. http://dx.doi.org/10.1534/genetics.104.039263

Hills MJ, Hall LM, Messenger DF, Graf RJ, Beres BL, Eudes F, 2007. Evaluation of crossability between triticale (× Triticosecale Wittmack) and common wheat, durum wheat and rye. Environ Biosafety Res 6(4): 249-257. http://dx.doi.org/10.1051/ebr:2007046

Ladizinsky G, 2000. A synthetic hexaploid (2n = 42) oat from the cross of Avena strigosa (2n = 14) and domesticated A. magna (2n = 28). Euphytica 116: 231-235. http://dx.doi.org/10.1023/A:1004056315278

Liu LW, Wang Y, Gong YQ, Zhao YM, Liu G, Li XY, Yu FM, 2007. Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrids using molecular markers. Sci Hortic 115: 7-12. http://dx.doi.org/10.1016/j.scienta.2007.07.013

Longin CFH, Mühleisen J, Maurer HP, Zhang H, Gowda M, Reif JCh, 2012. Hybrid breeding in autogamous cereals. Theor Appl Genet 125: 1087-1096. http://dx.doi.org/10.1007/s00122-012-1967-7

Nagaoka T, Ogihara Y, 1997. Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94: 597-602. http://dx.doi.org/10.1007/s001220050456

Nalepa S, 1990. Hybrid triticale: present and future. Proc 2nd Int Triticale Symp Passo Fundo, Rio Grande do Sul, Brasil, 1-5 October, CIMMYT, Mexico, pp: 402-407.

Nalepa S, 2003. Perspektywy hodowli pszenżyta w Resource Seeds Inc. w USA. Bull IHAR 230: 143-146.

Naresh V, Yamini KN, Rajendrakumar P, Dinesh Kumar V, 2009. EST-SSR marker based assay for the genetic purity assessment of safflower hybrids. Euphytica 170: 347-353. http://dx.doi.org/10.1007/s10681-009-9995-3

Stepansky A, Kovalski I, Perl-Treves R, 1999. Intraspecific classification of melons (Cucumis melo L.) in view of their phenotypic and molecular variation. Plant Syst Evol 217: 313-333. http://dx.doi.org/10.1007/BF00984373

Tarkowski CZ, Otłowska D, 1968. Badania nad heksaploidalnym triticale i jego mieszańcami z żytem i pszenicą [Studies on hexaploid triticale and its hybrids with rye and wheat]. Hodowla Roślin [Plant Breeding] 5: 577-593.

Warzecha R, Salak-Warzecha K, Staszewski Z, 1998. Development and use of triticale CMS system in hybrid breeding. Proc 4th Int Triticale Symp Red Deer, Alberta, Canada, July 26-31, pp: 79-85.

Published
2014-11-04
How to Cite
Warzecha, T., Sutkowska, A., & Góral, H. (2014). Male sterility of triticale lines generated through recombination of triticale and rye maintainers. Spanish Journal of Agricultural Research, 12(4), 1124-1130. https://doi.org/10.5424/sjar/2014124-6144
Section
Plant breeding, genetics and genetic resources