Short communication: Aqueous-acetone extraction improves the drawbacks of using dimethylsulfoxide as solvent for photometric pigment quantification in Quercus ilex leaves

  • Rosario Gonzalez-Cascon INIA, Dept. Environment. Ctra. Coruña km 7.5, 28040 Madrid http://orcid.org/0000-0003-3468-0967
  • Leticia Jiménez-Fenoy INIA, Dept. Environment. Ctra. Coruña km 7.5, 28040 Madrid
  • Irene Verdú-Fillola INIA, Dept. Environment. Ctra. Coruña km 7.5, 28040 Madrid
  • M. Pilar Martín CSIC, Center for Human and Social Sciences, Environmental Remote Sensing and Spectroscopy Laboratory. Albasanz 26-28, 28037 Madrid
Keywords: chlorophyll, carotenoids, DMSO, SPAD-502, phenolic compounds

Abstract

Aim of study: We evaluated the use of dimethylsulfoxide (DMSO) for the photometric determination of chlorophyll (Chla and Chlb) and carotenoids in Quercus ilex L. leaves by comparative analysis using aqueous-acetone extraction.

Area of study: a Q. ilex dehesa in Las Majadas del Tietar, Cáceres, Spain

Material and methods: Q. ilex leaves were sampled during two vegetative periods. Field SPAD-502 Chlorophyll measurements and photometric chlorophyll determinations were performed.  Two procedures were used: extraction of intact foliar discs at 65ºC with DMSO and fine foliar powder with cold aqueous-acetone.

Main results: DMSO produced Chlb overestimation and different fitting performance for SPAD vs pigment calibrations (R2=0.64, RMSE=0.20, p<0.0001 for Chla (µg/cm2); R2=0.33, RMSE=0.23, p<0.0001 for Chlb (µg/cm2) and R2=0.50, RMSE=0.23, p<0.0001 for carotenoids (µg/cm2)). Aqueous-acetone provided more accurate predictions (R2=0.90, RMSE=0.16, p<0.0001 for Chla and R2=0.91, RMSE=0.16, p<0.0001 for Chlb, R2=0.90, RMSE=0.02, p<0.0001 for carotenoids) and mean ratio Chla/Chlb=3.6 inside the range for sun exposed leaves.

Research highlights: Oxidizing conditions and polyphenol concentrations in Q. ilex leaves generated brown colorations in the DMSO extraction procedure, interfering with the photometric measurements in the red-orange region. Aqueous-acetone extraction was free from interference. DMSO should be avoided for pigment determination in Q. ilex leaves or when comparing different tree species.

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Author Biography

Rosario Gonzalez-Cascon, INIA, Dept. Environment. Ctra. Coruña km 7.5, 28040 Madrid

 

 

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Published
2017-10-20
How to Cite
Gonzalez-CasconR., Jiménez-FenoyL., Verdú-FillolaI., & MartínM. P. (2017). Short communication: Aqueous-acetone extraction improves the drawbacks of using dimethylsulfoxide as solvent for photometric pigment quantification in Quercus ilex leaves. Forest Systems, 26(2), eSC04. https://doi.org/10.5424/fs/2017262-11099
Section
Short communications