Influence of low temperature and frost duration on Phytophthora alni subsp. alni viability
AbstractLimits on the survival of P. alni subsp. alni (PAA) due to low temperature can be expected based on previously published laboratory and field studies. This study presents a laboratory experiment to test the influence of low temperature and frost duration on PAA viability. Ten PAA isolates were incubated at different temperatures (–0.1, –2.5, –5.0, –7.5, and –10.0 °C) and frost durations (0 – 7, 14, 21, and 28 days). A regression analysis confirmed the significant influence of both factors (low temperature and frost duration, and their interaction) on the survival of the pathogen under laboratory conditions. The survival and failure time analysis showed that the survival of the pathogen differs significantly after mild frost (all the isolates tested survived temperatures between –0.1 and –5.0 °C during the entire testing period) and heavy frost (the pathogen died after 21 days of incubation at –7.5 °C and after 2 days at –10.0 °C). Moreover, the viability of the pathogen decreased significantly if the temperature of –5.0 °C was maintained for at least 1 week and the temperature of –7.5 °C persisted in laboratory conditions for at least 4 days. The results of the study proved the pathogen to be very sensitive to heavy frost. The low-temperature limits for PAA occur regularly in Central Europe in January. It is probable that these temperatures can reduce PAA populations in diseased black alder stems. The climate change characterised by increases in the lowest minimum winter temperatures in Central Europe (as hypothesised by IPCC) may pose a significant risk for affected alder population in the area.
Bergot M., Cloppet E., Pérarnaud V., Déqué M., Marçais B., Desprez-Loustau M.-L., 2004. Simulation of potential range expansion of oak disease caused by Phytophthora cinnamomi under climate change. Global Change Biol 10, 1539-1552.
Bonate P.L., 2000. Analysis of pretest-posttest designs. Chapman & Hall/CRC, Boca Raton, USA. 205 pp.
Brasier C.M., Kirk S.A., Delcan J., Cooke D.E.L., Jung T., Man in’t Veld W.A., 2004. Phytophthora alni sp. nov. and its variants: designation of emerging heteroploid hybrid pathogen spreading on Alnus trees. Mycol Res 108, 1172-1184.
Brasier C.M., Rose J., Gibbs J.N., 1995. An unusual Phytophthora associated with widespread alder mortality in Britain. Plant Pathol 44, 999-1007.
černý k., strnadová V., 2010. Phytophthora alder decline: disease symptoms, causal agent and its distribution in the Czech Republic. Plant Protect Sci 46, 12-18.
Černý K., Strnadová V., accepted. Winter survival of Phytophthora alni subsp. alni in aerial tissues of black alder. J For Sci.
Coakley S.M., Scherm H., 1996. Plant disease in a changing global environment. Asp Appl Biol 45, 227-238.
Delcán J., Brasier C.M., 2001. Oospore viability and variation in zoospore and hyphal tip derivates of the hybrid alder Phytophthoras. For Path 31, 65-83.
Downing M.C., Jung T., Thomas V.L., Blaschke M., Tuffly M.F., Reich R.M., 2008. Estimating the susceptibility to Phytophthora alni globally using both statistical analyses and expert knowledge [online]. Available in http://www.forestencyclopedia.net/p/p5/p3389/p3328. [1 May 2011].
Erwin D.C., Ribeiro O.K., 1996. Phytophthora Diseases Worldwide. APS Press. St. Paul. USA. 562 pp.
Gibbs J.N., Lipscombe M.A., Peace A.J., 1999. The impact of Phytophthora disease on riparian populations of common alder (Alnus glutinosa) in southern Britain. Eur J For Pathol 29, 39-50.
Ioos R., Andrieux A., Marcais B, Frey P., 2006. Genetic characterization of the natural hybrid species Phytophthora alni as inferred from nuclear and mitochondrial DNA analyses. Fung Genet Biol 43, 511-529.
Intergovernmental Panel on Climate Change, (2007) Climate change 2007: the physical science basis. In Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, UK and New York, USA. 996 pp.
Jung T., Blaschke M., 2004. Phytophthora root and collar rot of alders in Bavaria: distribution, modes of spread and possible management strategies. Plant Pathol 53, 197-208.
Jung T., Blaschke M., 2006. Management strategies for the Phytophthora root and collar rot epidemic of alders in Bavaria. Proc III IUFRO Int Conf on “Progress in Research on Phytophthora Diseases of Forest Trees”, Freising (Germany), September 11-18. pp. 61-66.
Munda A., Žerjav M., Jakša J., 2006. Occurrence and characterization of alder Phytophthora, Phytophthora alni, in Slovenia. Proc III IUFRO Int Conf on “Progress in Research on Phytophthora Diseases of Forest Trees”, Freising (Germany), September 11-18. CD ROM.
Oszako T., Orlikowski L., 2005. Phytophthora alni as the main cause of the alder decline in Poland. Prog Plant Prot 45, 343-350.
Schumacher J., 2006. Spread of alder Phytophthoras in the Spreewald biosphere reserve and a survey of alder nurseries in Brandenburg. Proc III IUFRO Int Conf on “Progress in Research on Phytophthora Diseases of Forest Trees”, Freising (Germany), September 11-18. pp. 55-60.
Schumacher J., Leonhard S., Grundmann B.M., Roloff A., 2006. New alder disease in Spreewald biosphere reserve – causes and incidental factors of an epidemic. Nachrichtenbl Deut Pflanzenschutzd 58, 141-147.
Streito J.-C., Legrand P., Tabary F., Jarnouen de Villartay G., 2002. Phytophthora disease of alder (Alnus glutinosa) in France: investigations between 1995 and 1999. For Path 32, 179-191.
Tolasz R., Brázdil R., Bulíř O., Dobrovolný P., Dubrovský M., Hájková M, et al., 2007. Climate Atlas of Czechia. CHMI, Praha, UPOL, Olomouc, Czech Rep. 255 pp.
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