Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

  • María F. Pomponio Instituto de Recursos Biológicos (IRB), CIRN, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
  • Cintia Acuña Instituto de Biotecnología (IB), CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
  • Vivien Petreath 3Universidad Nacional de Patagonia San Juan Bosco, Comodoro Rivadavia-Chubut.
  • Diego L. Lauenstein Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), Instituto Nacional de Tecnología Agropecuaria (INTA), Córdoba.
  • Susana M. Poltri Instituto de Biotecnología (IB), CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
  • Susana Torales Instituto de Recursos Biológicos (IRB), CIRN, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).

Abstract

Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus.

Area of the study: samples were obtained from natural populations of Argentina.

Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids.

Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci.

Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.

Keywords: genetic variation; functional markers; microsatellites; prosopis.

Abbreviations: PIC: Polymorphic Information Content; PCR: Polymerase Chain Reaction; SSR: Simple Sequence Repeat.

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Author Biographies

María F. Pomponio, Instituto de Recursos Biológicos (IRB), CIRN, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
Instituto de Recursos Biológicos (IRB)
Cintia Acuña, Instituto de Biotecnología (IB), CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
Instituto de Biotecnología (IB), CICVyA
Diego L. Lauenstein, Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), Instituto Nacional de Tecnología Agropecuaria (INTA), Córdoba.
Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV)
Susana M. Poltri, Instituto de Biotecnología (IB), CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
Instituto de Biotecnología (IB), CICVyA
Susana Torales, Instituto de Recursos Biológicos (IRB), CIRN, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar).
Instituto de Recursos Biológicos (IRB)

References

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Published
2015-07-27
How to Cite
PomponioM. F., AcuñaC., PetreathV., L. LauensteinD., M. PoltriS., & ToralesS. (2015). Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species. Forest Systems, 24(2), eRC04. https://doi.org/10.5424/fs/2015242-07188
Section
Resource communications