Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores

Keywords: Nanoparticles, rhizoid, prothallus, chloroplast, fern.


Aim of study: The effect of cerium oxide engineered nanoparticles on the spore germination of the fern. Asplenium adiantum-nigrum.

Area of study: France, Britanny Region, Finistére Department, Plougonvelin, in rocks near the sea.

Material and methods: Asplenium spores were cultured in vitro on agar medium with Nano-CeO2 (less than 25 nm particle size) and bulk-CeO2. The addition of each nano- and bulk particles ranged from 0 to 3000 mg L-1. Observations on rhizoidal and prothallial cells during first stages of gametophyte development were made. The No-Observed-Adverse-Effect concentration (NOAEC) and Lowest-Observed-Adverse-Effect-Concentration (LOEC) values for spore germination rate data were analyzed.

 Main results: Germination was speeded up by 100 to 2000 mg L-1 nanoceria, while bulk cerium oxide had the same effect for 500 to 200 mg L-1 concentrations. Present results showed cellular damage in the protonema while rhizoid cells seemed not to be affected, as growth and membrane integrity remained.

Research highlights: Both nanosized and bulk cerium oxide are toxic for the fern Asplenium adiantum-nigrum, although diverse toxicity patterns were shown for both materials. Diverse toxic effects have been observed: chloroplast membrane damage and lysis, cell wall and membrane disruption which leads to cell lysis; and alterations in morphology and development.

Keywords: Nanoparticles; rhizoid; prothallus; chloroplast; fern.


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Author Biographies

Aranzazu Gomez-Garay, Facultad de Biología. Universidad Complutense de Madrid (UCM). Madrid.
Departamento de Biología Vegetal I: Botánica y Fisiología Vegetal.
Beatriz Pintos, Facultad de Biología. Universidad Complutense de Madrid (UCM). Madrid.
Departamento de Biología Vegetal I: Botánica y Fisiología Vegetal.
José Antonio Manzanera, College of Forestry and Natural Environment, Technical University of Madrid (UPM), CEI Moncloa, Madrid.
Research Group for Sustainable Management SILVANET
Carmen Prada, Facultad de Biología. Universidad Complutense de Madrid (UCM). Madrid.
Departamento de Biología Vegetal I: Botánica y Fisiología Vegetal.
Luisa Martin, Facultad de Biología. Universidad Complutense de Madrid (UCM). Madrid.
Departamento de Biología Vegetal I: Botánica y Fisiología Vegetal.
José María Gabriel y Galan, Facultad de Biología. Universidad Complutense de Madrid (UCM). Madrid.
Departamento de Biología Vegetal I: Botánica y Fisiología Vegetal.



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How to Cite
Gomez-GarayA., PintosB., ManzaneraJ. A., PradaC., MartinL., & Gabriel y GalanJ. M. (2016). Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores. Forest Systems, 25(3), e067.
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