In vitro fermentation pattern and acidification potential of different sources of carbohydrates for ruminants given high concentrate diets

  • Zahia Amanzougarene Universidad de Zaragoza-CITA, Instituto Agroalimentario de Aragón (IA2), Dept. Producción Animal y Ciencia de los Alimentos. M. Servet 177, 50013 Zaragoza
  • Susana Yuste Universidad de Zaragoza-CITA, Instituto Agroalimentario de Aragón (IA2), Dept. Producción Animal y Ciencia de los Alimentos. M. Servet 177, 50013 Zaragoza
  • Antonio de Vega Universidad de Zaragoza-CITA, Instituto Agroalimentario de Aragón (IA2), Dept. Producción Animal y Ciencia de los Alimentos. M. Servet 177, 50013 Zaragoza
  • Manuel Fondevila Universidad de Zaragoza-CITA, Instituto Agroalimentario de Aragón (IA2), Dept. Producción Animal y Ciencia de los Alimentos. M. Servet 177, 50013 Zaragoza
Keywords: incubation pH, gas production, sugarbeet pulp, sucrose, citrus pulp, wheat bran


The in vitro fermentation pattern of five sources of carbohydrates of differing nature (maize grain, MZ; sucrose, SU; wheat bran, WB; sugarbeet pulp, BP; and citrus pulp, CT) under conditions of high concentrate diets for ruminants was studied. A first 8 h incubation trial was performed under optimal pH using inoculum from ewes given a fibrous diet, to compare fermentative characteristics of substrates. As planned, incubation pH ranged within 6.3 to 6.6. The gas produced from CT was higher than MZ, SU and BP from 4 and 6 h onwards, and at 8 h, respectively (p<0.05). There were no differences (p>0.05) on total volatile fatty acid (VFA) concentration, nor on acetate or propionate proportions, but butyrate was lowest (p<0.05) with CT and BP. The second incubation trial was performed in a poorly-buffered medium, with inoculum from ewes given a concentrate diet. All substrates showed a gradual drop of pH, being lowest with SU after 4 h (p<0.05). Throughout the incubation, gas production was highest with CT and lowest with MZ and BP (p<0.05). Total 8 h VFA concentration was higher with CT than BP, SU and MZ (p<0.05). Acetate proportion was higher, and that of propionate lower, with BP than WB (p<0.05), butyrate proportion being higher with MZ and WB than with BP and CT (p<0.05). Lactic acid concentration was higher (p<0.05) with SU than WB and BP. Fermentation characteristics and acidification potential of feeds depend on the nature of their carbohydrate fraction, and must be considered for practical applications.


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How to Cite
AmanzougareneZ., YusteS., de VegaA., & FondevilaM. (2017). In vitro fermentation pattern and acidification potential of different sources of carbohydrates for ruminants given high concentrate diets. Spanish Journal of Agricultural Research, 15(2), e0602.
Animal production