Inhibitory action of essential oils against proteases activity of Paenibacillus larvae, the etiological agent of American Foulbrood disease

  • María C. Pellegrini 1 Universidad Nacional de Mar del Plata (UNMdP), Facultad de Ciencias Exactas y Naturales, Centro de Investigación en Abejas Sociales (CIAS), Microbiología Aplicada. 7600 Mar del Plata 2 Comisión de Investigaciones Científicas (CIC). 1900 La Plata
  • Lucía Zalazar 3 UNMdP, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas (IIB) CONICET, Biología de Procariotas y Gametas. 7600 Mar del Plata 4 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). 1425 CABA
  • Sandra R. Fuselli 1 Universidad Nacional de Mar del Plata (UNMdP), Facultad de Ciencias Exactas y Naturales, Centro de Investigación en Abejas Sociales (CIAS), Microbiología Aplicada. 7600 Mar del Plata 2 Comisión de Investigaciones Científicas (CIC). 1900 La Plata
  • Alejandra G. Ponce 4 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). 1425 CABA 5 UNMdP, Facultad de Ingeniería. Ingeniería en Alimentos. 7600 Mar del Plata
Keywords: virulence factor, proteases, spent medium, gram positive, bee infection


American foulbrood (AFB) is a disease affecting the larva of Apis mellifera. The etiological agent is Paenibacillus larvae, which releases metalloproteases involved in the degradation of larval tissues. Through quorum sensing (QS) mechanism, bacteria are able to activate specific genes such as virulence factors. The exoproteases regulation of P. larvae could be associated with QS. A promising mechanism of AFB control is to block QS mechanism with essential oils (EO). The aim of this study was to investigate the potential presence of QS signals in the regulation of P. larvae proteases and the effect of seven EOs on the exoproteases activity of P. larvae. From growth curves and evaluation of the presence of proteases by milk agar plates assay, it was observed protease activity during the late exponential phase of growth. Early production of protease activity (15 hours earlier than control) was observed when a low density culture was incubated with late exponential spent medium (SM) suggesting the presence of factor(s) inducing this activity. SM was obtained by the ultrafiltration of P. larvae cultures on late growth phase and was free of proteases. Proteolytic activity was quantified on P. larvae cultures in presence of sublethal concentration of EO by azocasein method. The EOs, except S. chilensis EO, reduced significantly protease activity (more than 50%). We report for the first time evidence on the possible role of QS on P. larvae and the antiproteolytic activity of EOs (except for S. chilensis) on exoproteases, an interesting therapeutic strategy to control AFB.


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How to Cite
PellegriniM. C., ZalazarL., FuselliS. R., & PonceA. G. (2018). Inhibitory action of essential oils against proteases activity of Paenibacillus larvae, the etiological agent of American Foulbrood disease. Spanish Journal of Agricultural Research, 15(4), e0504.
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