Genetic redundancy among durum wheat accessions as assessed by SSRs and endosperm proteins

  • M. Ruiz Centro de Recursos Fitogenéticos (CRF). INIA. Alcalá de Henares (Madrid)
  • E. Aguiriano Centro de Recursos Fitogenéticos (CRF). INIA. Alcalá de Henares (Madrid)
  • P. Giraldo ETSI Agrónomos. Universidad Politécnica de Madrid (UPM).
  • J. M. Carrillo ETSI Agrónomos. Universidad Politécnica de Madrid (UPM).
Keywords: cereals, duplicates, ex-situ collections, molecular data, prolamins


Reducing duplication in ex-situ collections is complicated and requires good quality genetic markers. This study was conducted to assess the value of endosperm proteins and SSRs for validation of potential duplicates and monitoring intra-accession variability. Fifty durum wheat (Triticum turgidum ssp. durum) accessions grouped in 23 potential duplicates, and previously characterised for 30 agro-morphological traits, were analysed for gliadin and high molecular weight glutenin (HMWG) subunit alleles, total protein, and 24 SSRs, covering a wide genome area. Similarity and dissimilarity matrices were generated based on protein and SSRs alleles. For heterogeneous accessions at gliadins the percent pattern homology (PH) between gliadin patterns and the Nei’s coefficient of genetic identity (I) were computed. Eighteen duplicates identical for proteins showed none or less than 3 unshared SSRs alleles. For heterogeneous accessions PH and I values lower than 80 identified clearly off-types with more than 3 SSRs unshared. Only those biotypes differing in no more than one protein-coding locus were confirmed with SSRs. A good concordance among proteins, morphological traits, and SSR were detected. However, the discrepancy in similarity detected in some cases showed that it is advisable to evaluate redundancy through distinct approaches. The analysis in proteins together with SSRs data are very useful to identify duplicates, biotypes, close related genotypes, and contaminations.


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How to Cite
RuizM., AguirianoE., GiraldoP., & CarrilloJ. M. (1). Genetic redundancy among durum wheat accessions as assessed by SSRs and endosperm proteins. Spanish Journal of Agricultural Research, 9(1), 156-165.
Plant breeding, genetics and genetic resources