Short communication. Genetic diversity within Scorpiurus species from the Iberian Peninsula estimated using ITS DNA sequences

  • T. Visnevschi-Necrasov CIBIO-ICETA. Vairão
  • D. J. Harris CIBIO-ICETA. Vairão
  • M. A. Faria REQUIMTE. Laboratório de Bromatologia e Hidrologia. Faculty of Pharmacy. University of Porto
  • G. Pereira INRB/INIA. Elvas.
  • E. Nunes CIBIO-ICETA. Vairão
Keywords: forage, prickly caterpillar, Scorpiurus muricatus, Scorpiurus sulcatus, Scorpiurus vermiculatus


Scorpiurus muricatus and Scorpiurus vermiculatus have been proposed as possible subjects for breeding programs to improve their potential as forage crops. However, prior to this genetic diversity between these species and within distinct populations should be assessed. We estimated diversity between populations from the Iberian Peninsula using ITS 1 and ITS 2 nuclear DNA sequences. Twelve haplotypes from 25 individuals were determined, and a network of relationships produced. Our results indicate considerable haplotypic diversity, but no deep subdivisions either within S. muricatus or between the two species. This means that no potential barriers to the proposed breeding programs were identified.


Download data is not yet available.


Abbate V., Maugeri G., Cristaudo A., Gresta F., 2010. Scorpiurus muricatus L. subsp. Subvillosus (L.) Thell., a potential forage legume species for a Mediterranean environment: a review. Grass Forage Sci 65, 2-10.

Allan G.J., Porter J.M., 2000. Tribal delimitation and phylogenetic relationships of Loteae and Coronilleae (Faboideae: Fabaceae) with special reference to Lotus: evidence from nuclear ribosomal ITS sequences. Am J Bot 87, 1871-1881. PMid:11118424

Chen S.L., Yao H., Han J.P., Liu C., Song J.Y., Shi L.C., Zhu Y.J., Ma X.Y., Gao T., Pang X.H., Luo K., Li Y., Li X.W., Jia X.C., Lin Y.L., Leon C., 2010. Validation of the ITS 2 region as a novel DNA barcode for identifying medicinal plant species. PLOS One 5, e8613. PMid:20062805 PMCid:2799520

Clement M., Posada D., Crandall K.A., 2000. TCS: a computer program to estimate gene genealogies. Mol Ecol 9, 1657-1660. PMid:11050560

Díez M., Ferguson I. K., 1996. Studies of the pollen morphology and taxonomy of the tribes Loteae and Coronilleae (Papilionoideae; Leguminoseae). 3, Coronilla L. and related genera and systematic conclusions. Rev Palaeobot Palyno 94, 239-257.

Domínguez E., Galiano E.F., 1974. Revisión del género Scorpiurus L. II. Parte sistematica. Lagascalia 4, 259-280. [In Spanish].

Hall T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Series 41, 95-98.

Heyn C.C., Raviv V., 1966. Experimental taxonomic studies in the genus Scorpiurus (Papilionaceae). Bulletin of the Torrey Botanical Club 93, 259-267.

Linnaeus C., 1753. Species plantarum, Vol II. Stockholm, Sweden.

Wang X.D., Wang Z.P., Zou Y.P., 1996. An improved procedure for the isolation of nuclear DNA from leaves of wild grapevine dried with silica gel. Plant Mol Biol Rep 14, 369-373.

White T.J., Bruns T.D., Lee S.B., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR – protocols and applications – a laboratory manual. Academic Press, New York.

Zieliński J., 1991. Scorpiurus vermiculatus (Fabaceae) rediscovered in Greece. Willdenowia 20, 39-41.

How to Cite
Visnevschi-NecrasovT., HarrisD. J., FariaM. A., PereiraG., & NunesE. (2011). Short communication. Genetic diversity within Scorpiurus species from the Iberian Peninsula estimated using ITS DNA sequences. Spanish Journal of Agricultural Research, 9(1), 198-201.
Plant breeding, genetics and genetic resources