Short communication. Response of bacterial community composition to long-term applications of different composts in agricultural soils

  • M. Ros Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC). Espinardo (Murcia)
  • B. A. Knapp University of Innsbruck. Institut für Mikrobiologie. Innsbruck
  • U. Peintner University of Innsbruck. Institut für Mikrobiologie. Innsbruck
  • H. Insam University of Innsbruck. Institut für Mikrobiologie. Innsbruck
Keywords: 16S rRNA clone libraries, fertilizer, isolated bacteria, organic amendments


Differences in the bacterial community composition of agricultural soils caused by a long-term (12 year) application of different composts were identified by cultivation-dependent and -independent methods (PCR-DGGE and 16S rRNA clone libraries). The number of colony forming units indicated that the successive incorporation of organic amendments increased the bacterial abundance (6.41-5.66 log10 cfu g–1dry soil) compared to control and mineral soils (5.54-3.74 log10 cfu g–1 dry soil). Isolated bacteria were dominated by Actinobacteria, whereby compost-amended soils and green compost-amended soils showed, respectively, higher number of members of Actinobacteria (100% and 64%) than control and mineral soils (50% and 40%). The 16S rRNA clone libraries were dominated by Proteobacteria (43%), Acidobacteria (21%) and Actinobacteria (13%). Proteobacteria and Actinobacteria were most abundant in compost amended soils while Acidobacteria were more frequently found in mineral fertilizer and control soils. Partial 16S rRNA gene clone libraries revealed a higher bacterial diversity than cultivation. In conclusion, we found differences of bacterial community composition with a cultivation approach and clone libraries between compost amended soils and control and mineral soil.


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Author Biography

M. Ros, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC). Espinardo (Murcia)

Department of Soil and Water Conservation and Organic Waste Management


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How to Cite
RosM., KnappB. A., PeintnerU., & InsamH. (1). Short communication. Response of bacterial community composition to long-term applications of different composts in agricultural soils. Spanish Journal of Agricultural Research, 9(1), 338-343.
Soil science