Phenolic profile of grapevine cv. Tempranillo skins is affected by timing and severity of early defoliation

  • Daniel Moreno Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX). Instituto Tecnológico Agroalimentario de Extremadura (INTAEX). Avda. Adolfo Suárez, s/n. 06071 Badajoz
  • Diego S. Intrigliolo IVIA. Centro para el Desarrollo de la Agricultura Sostenible. Ctra. CV-315 km 10.7, 46113-Moncada, Valencia IVIA. Centro para el Desarrollo de la Agricultura Sostenible. Ctra. CV-315 km 10.7, 46113-Moncada, Valencia
  • Mar Vilanova CSIC-ICVV. Instituto de Ciencias de la Vid y del Vino. Finca "La Grajera", Ctra. 16 Burgos km 6, 26080 Logroño, La Rioja
  • Juan R. Castel IVIA. Centro para el Desarrollo de la Agricultura Sostenible. Ctra. CV-315 km 10.7, 46113-Moncada, Valencia
  • Esther Gamero Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX). Instituto Tecnológico Agroalimentario de Extremadura (INTAEX). Avda. Adolfo Suárez, s/n. 06071 Badajoz
  • Esperanza Valdés Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX). Instituto Tecnológico Agroalimentario de Extremadura (INTAEX). Avda. Adolfo Suárez, s/n. 06071 Badajoz
Keywords: anthocyanins, flavanols, flavonols, hydroxycinnamic acids

Abstract

Aim of study: To investigate the effects of three early leaf removal treatments on the phenolic compounds of cv. ‘Tempranillo’ (Vitis vinifera L.) grape skins.

Area of study: The experiment was conducted in a vineyard located in Requena, Valencia (South-eastern Spain) over two consecutive seasons.

Materials and methods: Four treatments were investigated over two seasons in drip-irrigated vines: Control (C), non-defoliated and three defoliation treatment, applied at different phenological stages and intensities where all leaves from the first 6 nodes were eliminated just before flowering (ED) and at fruitset (LD). The fourth defoliation treatment was performed at the same time of ED but only the leaves facing east of the eight first nodes were removed (EED). At harvest, thirty-eight phenolic compounds were quantified by HPLC in the grape skins, including anthocyanins, flavanols, flavonols, hydroxycinnamic acids and their tartaric derivatives.

Main results: A general increase of the skin phenolic compounds concentration was found in response to the defoliation treatments. The largest and more significant effects were observed for LD in 2009 with relative increases with respect to the un-defoliated vines of 14.8, 86.0, 119.0, and 75.9% for anthocyanins, flavanols, flavonols and hydroxycinnamates, respectively. On the other hand, EED did not clearly modify any polyphenolic compound. In addition, the response of phenolic families analyzed to defoliation treatments was different. Malvidine derivatives were not altered by any of the treatments, while the contents of quercetin and kaempferol derivatives and ferulic and coumaric acids, increased in both years when LD was applied.

Research highlights: The defoliation effects on specific phenolic substances were dependent on timing, severity, and the season. Skin phenolic compounds increase in response to defoliation treatments and flavonols and hydroxycinnamates were the most affected families.

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References

Acimovic D, Tozzini L, Green A, Sivilotti P, Sabbatini P, 2016. Identification of a defoliation severity threshold for changing fruitset, bunch morphology and fruit composition in Pinot Noir. Aust J Grape Wine Res 22 (3): 399-408. https://doi.org/10.1111/ajgw.12235

Baggiolini M, 1952. Les stades repérés dans le développement annuel de la vigne et leur utilisation pratique. Rev Rom Agric Vitic Arboric 8: 4-6.

Bergqvist J, Dokoozlian N, Ebisuda N, 2001. Sunlight exposure and temperature effects on berry growth and composition of Cabernet Sauvignon and Grenache in the Central San Joaquin Valley of California. Am J Enol Vitic 52 (1): 3-9.

Bogicevic M, Maras V, Mugosa M, Kodzulovic V, Raicevic J, Sucur S, Failla O, 2015. The effects of early leaf removal and cluster thinning treatments on berry growth and grape composition in cultivars Vranac and Cabernet Sauvignon. Chem Biol Technol Agric 2:13. https://doi.org/10.1186/s40538-015-0037-1

Boulton R, 2001. The copigmentation of anthocyanins and its role in the color of red wine: A critical review. Am J Enol Vitic 52 (2): 67-87.

Chorti E, Guidoni S, Ferrandino A, Novello V, 2010, Effect of different cluster sunlight exposure levels on ripening and anthocyanin accumulation in Nebbiolo grapes. Am J Enol Viticult 61 (1): 23-30.

Degu A, Ayenew B, Cramer GR, Fait A, 2016. Polyphenolic responses of grapevine berries to light, temperature, oxidative stress, abscisic acid and jasmonic acid show specific developmental-dependent degrees of metabolic resilience to perturbation. Food Chem 212: 828-836. https://doi.org/10.1016/j.foodchem.2016.05.164

Diago MP, Vilanova M, Tardaguila J, 2010. Effects of timing of manual and mechanical early defoliation on the aroma of Vitis vinifera L. Tempranillo wine. Am J Enol Vitic 61 (3): 382-391.

Diago MP, Ayestarán B, Guadalupe Z, Garrido A, Tardáguila J, 2012. Phenolic composition of Tempranillo wines following early defoliation of the vines. J Sci Food Agric 92 (4): 925-34. https://doi.org/10.1002/jsfa.4671

Dokoozlian NK, Kliewer WM, 1996. Influence of light on grape berry growth and composition varies during fruit development. J Am Soc Hortic Sci 121 (5): 869-874. https://doi.org/10.21273/JASHS.121.5.869

Downey MO, Dokoozlian NK, Krstic MP, 2006. Cultural practice and environmental impacts on the flavonoid composition of grapes and wine: A review of recent research. Am J Enol Vitic 57 (3): 257-268.

Falginella L, Castellarin SD, Testolin R, Gambetta, GA, Morgante M, Di Gaspero, G, 2010. Expansion and subfunctionalisation of flavonoid 3′,5′-hydroxylases in the grapevine lineage. BMC Genomics 11 (1): 562. https://doi.org/10.1186/1471-2164-11-562

Gamero E, Espinosa F, Moreno D, Uriarte D, Prieto MH, Garrido I, Valdés E, 2018. Convenience of applying of viticulture technique as a function of the water status of the vine-stock. Grapes and wines In: Grapes and wines: advances in production, processing, analysis and valorization. Jordao AM, Cosme F (eds). IntechOpen. pp: 91-116. https://doi.org/10.5772/intechopen.72799

Gao L, Girard B, Mazza G, Reynolds AG, 1997. Changes in anthocyanins and color characteristics of Pinot Noir wines during different vinification processes. J Agric Food Chem 45 (6): 2003-2008. https://doi.org/10.1021/jf960836e

García-Estévez I, Andrés-García P, Alcalde-Eon C, Giacosa S, Rolle L, Rivas-Gonzalo JC, et al., 2015. Relationship between agronomic parameters, phenolic composition of grape skin, and texture properties of Vitis vinifera L. cv. Tempranillo. J Agric Food Chem 63 (35): 7663-7669. https://doi.org/10.1021/acs.jafc.5b00275

Gatti M, Bernizzoni F, Civardi S, Poni S, 2012. Effects of cluster thinning and preflowering leaf removal on growth and grape composition in cv. Sangiovese. Am J Enol Vitic 63 (3): 325-332. https://doi.org/10.5344/ajev.2012.11118

Giovanelli G, Brenna OV, 2007. Evolution of some phenolic components, carotenoids and chlorophylls during ripening of three Italian grape varieties. Eur Food Res Technol 225 (1): 145-150. https://doi.org/10.1007/s00217-006-0436-4

González-Manzano S, Santos-Buelga C, Dueñas M, Rivas-Gonzalo JC, Escribano-Bailón T, 2008. Colour implications of self-association processes of wine anthocyanins. Eur Food Res Technol 226 (3): 483-490. https://doi.org/10.1007/s00217-007-0560-9

Gouot J, Smith J, Holzapfel B, Barril C, 2020. A summary of the effect of high temperature on red grape flavonoid composition and biosynthesis. Wine Vitic J 35 (1): 35-36, 38-41.

Guidoni S, Ferrandino A, Novello V, 2008. Effects of seasonal and agronomical practices on skin anthocyanin profile of Nebbiolo grapes. Am J Enol Vitic 59 (1): 22-29.

He F, Liang NN, ML, Pan QH, Wang J, Reeves MJ, Duan CQ, 2012. Anthocyanins and their variation in red wines I. Monomeric anthocyanins and their color expression. Mol 17 (2): 1571-601. https://doi.org/10.3390/molecules17021571

Hed B, Ngugi HK, Travis JW, 2015. Short- and long-term effects of leaf removal and gibberellin on Chardonnay grapes in the Lake Erie region of Pennsylvania. Am J Enol Vitic 66 (1): 22-29. https://doi.org/10.5344/ajev.2014.14034

Huglin P, 1978. Nouveau mode d'évaluation des possibilités héliothermiques d'un milieu viticole. CR Acad Agric 64: 1117-1126.

Intrigliolo DS, Llacer E, Revert J, Esteve MD, Climent MD, Palau D, Gómez I, 2014. Early defoliation reduces cluster compactness and improves grape composition in Mandó, an autochthonous cultivar of Vitis vinifera from southeastern Spain. Sci Hortic 167: 71-75. https://doi.org/10.1016/j.scienta.2013.12.036

Kliewer W, Antcliff A, 1970. Influence of defoliation, leaf darkening, and cluster shading on the growth and composition of sultana grapes. Am J Enol Vitic 21: 26-36.

Kliewer WM, Smart RE, 1989. Canopy manipulation for optimizing vine microclimate, crop yield, and composition of grapes. In: Manipulation of fruiting; Wright CJ (ed.). pp: 275-291. Butterworth, London. https://doi.org/10.1016/B978-0-408-02608-6.50023-0

Kotseridis Y, Georgiadou, A, Tikos P, Kallithraka S, Koundouras S, 2012. Effects of severity of post-flowering leaf removal on berry growth and composition of three red Vitis vinifera L. cultivars grown under semiarid conditions. J Agric Food Chem 60 (23): 6000-6010. https://doi.org/10.1021/jf300605j

Lemut MS, Trost K, Sivilotti P, Vrhovsek U, 2011. Pinot Noir grape colour related phenolics as affected by leaf removal treatments in the Vipava Valley. J Food Compos Anal 24 (6): 777-784. https://doi.org/10.1016/j.jfca.2011.03.003

Lemut MS, Sivilotti P, Franceschi P, Wehrens R, Vrhovsek U, 2013a. Use of metabolic profiling to study grape skin polyphenol behavior as a result of canopy microclimate manipulatio J Agric Food Chem 61 (37): 8976-8986. https://doi.org/10.1021/jf4030757

Lemut MS, Trost K, Sivilotti P, Arapitsas P, Vrhovsek U, 2013b. Early versus late leaf removal strategies for Pinot Noir (Vitis vinifera L.): effect on colour-related phenolics in young wines following alcoholic fermentation. J Sci Food Agric 93 (15): 3670-3681. https://doi.org/10.1002/jsfa.6193

Mabrouk H, Carbonneau A, 1996. A simple method for determination of grapevine Vitis vinifera L. leaf area. Prog Agric Vitic 113 (18): 392-398.

Markovic D, 2000. Spectrophotometric study of copigmentation of malvin with caffeic and ferulic acid. J Agr Food Chem 48 (11): 5530-5536. https://doi.org/10.1021/jf000038v

Moreno D, Vilanova M, Gamero E, Intrigliolo DS, Talaverano MI, Uriarte D, Valdés E, 2015. Effects of preflowering leaf removal on phenolic composition of cv. Tempranillo in semi-arid terroir of Western Spain. Am J Enol Vitic 66 (2): 204-211. https://doi.org/10.5344/ajev.2014.14087

Moreno D, Valdés E, Uriarte D, Gamero E, Talaverano I, Vilanova M, 2017. Early leaf removal applied in warm climatic conditions: Impact on Tempranillo wine volatiles. Food Res Int 98: 50-58. https://doi.org/10.1016/j.foodres.2016.09.017

OIV, 1990. Recueil des méthodes internationales d'analyse des vins et des mouts. Office International de la Vigne et du Vin, Paris.

Palliotti A, Gatti M, Poni S, 2011. Early leaf removal to improve vineyard efficiency: gas exchange, source-to-sink balance, and reserve storage responses. Am J Enol Vitic 62: 219-228. https://doi.org/10.5344/ajev.2011.10094

Peña-Neira A, Cáceres A, Pastenes C, 2007. Low molecular weight phenolic and anthocyanin composition of grape skins from cv. Syrah (Vitis vinifera L.) in the Maipo Valley (Chile): Effect of clusters thinning and vineyard yield. Food Sci Technol Int 13 (2): 153-158. https://doi.org/10.1177/1082013207077920

Poni S, Casalini L, Bernizzoni F, Civardi S, Intrieri C, 2006. Effects of early defoliation on shoot photosynthesis, yield components, and grape composition. Am J Enol Vitic 57 (4) 397-407.

Poni S, Bernizzoni F, Civardi S, Libelli N, 2009. Effects of pre-bloom leaf removal on growth of berry tissues and must composition in two red Vitis vinifera L. cultivars. Aust J Grape Wine Res 15 (2): 185-193. https://doi.org/10.1111/j.1755-0238.2008.00044.x

Risco D, Pérez D, Yeves A, Castel JR, Intrigliolo DS, 2014. Early defoliation in a temperate warm and semi-arid Tempranillo vineyard: vine performance and grape composition. Aust J Grape Wine Res 20 (1): 111-122. https://doi.org/10.1111/ajgw.12049

Roby G, Harbertson JF, Adams D, Matthews M, 2004. Berry size and vine water deficits as factors in winegrape composition: Anthocyanins and tannins. Aust J Grape Wine Res 10 (2): 100-107. https://doi.org/10.1111/j.1755-0238.2004.tb00012.x

Santesteban LG, Miranda C, Royo JB, 2011. Regulated deficit irrigation effects on growth, yield, grape quality and individual anthocyanin composition in Vitis vinifera L. cv. 'Tempranillo'. Agric Water Manag 98: 1171-1179. https://doi.org/10.1016/j.agwat.2011.02.011

Schwarz M, Wabnitz TC, Winterhalter P, 2003. Pathway leading to the formation of anthocyanin-vinylphenol adducts and related pigments in red wines. J Agric Food Chem 51 (12): 3682-3687. https://doi.org/10.1021/jf0340963

Silvestroni O, Lanari V, Lattanzi T,. Palliotti A, VanderWeide J, Sabbatin P, 2018. Canopy management strategies to control yield and grape composition of Montepulciano grapevines. Aust J Grape Wine Res 25 (1): 30-42. https://doi.org/10.1111/ajgw.12367

Smart R, Robinson M, 1991. Sunlight into wine: a handbook for winegrape canopy management. In: Sunlight into wine a Handb winegrape canopy management; 88 pp. Winetitles, Adelaide.

Spayd SE, Tarara JM, Mee DL, Ferguson JC, 2002. Separation of sunlight and temperature effects on the composition of Vitis vinifera cv. Merlot berries. Am J Enol Vitic 53 (3): 171-182.

Tardaguila J, Diago MP, Martinez de Toda F, Poni S, Vilanova M, 2008. Effects of timing of leaf removal on yield, berry maturity, wine composition and sensory properties of cv. Grenache grown under non irrigated conditions. J Int Sci Vigne Vin 42 (4): 221-229. https://doi.org/10.20870/oeno-one.2008.42.4.810

Tardaguila J, Martinez de Toda F, Poni S, Diago MP, 2010. Impact of early leaf removal on yield and fruit and wine composition of Vitis vinifera L. Graciano and Carignan. Am J Enol Vitic 61 (3): 372-381.

Tonietto J, Carbonneau A, 2004. A multicriteria climatic classification system for grape-growing regions worldwide. Agr Forest Meteorol 124 (1-2): 81-97. https://doi.org/10.1016/j.agrformet.2003.06.001

Torres N, Hilbert, G, Luquin, J, Goicoechea N, Antolín MC, 2017. Flavonoid and amino acid profiling on Vitis vinifera L.cv Tempranillo subjected to deficit irrigation under elevated temperatures. J Food Comp Anal 62: 51-62. https://doi.org/10.1016/j.jfca.2017.05.001

Uriarte D, Picón J, Mancha LA, Blanco J, Prieto MH, Moreno D, et al., 2012. Early defoliation of 'Tempranillo' grapevines in semi-arid terroirs of Spain. Acta Hortic 931: 299-306. https://doi.org/10.17660/ActaHortic.2012.931.33

VanderWeide, J, Medina-Meza, IG, Frioni, T, Sivilotti P, Falchi R, Sabbatini P, 2018. Enhancement of fruit technological maturity and alteration of the flavonoid metabolomic profile in Merlot (Vitis vinifera L.) by early mechanical leaf removal. J Agric Food Chem 66 (37): 9839-9849. https://doi.org/10.1021/acs.jafc.8b02709

Verdenal T, Zufferey V, Dienes-Nagy A, Gindro K, Belcher S, Lorenzini F, et al., 2017. Pre-flowering defoliation affects berry structure and enhances wine sensory parameters. OENO One 51 (3): 263-275. https://doi.org/10.20870/oeno-one.2017.51.2.1808

Vilanova M, Diago MP, Genisheva Z, Oliveira JM, Tardaguila J, 2012. Early leaf removal impact on volatile composition of Tempranillo wines. J Sci Food Agric 92 (4): 935-942. https://doi.org/10.1002/jsfa.4673

Yue XF, Ju YL, Tang ZZ, Zhao YM, Jiao XL, Zhang ZW, 2019. Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines. J Integr Agric 18 (9): 2052-2062. https://doi.org/10.1016/S2095-3119(19)62666-3

Published
2021-08-12
How to Cite
MorenoD., IntriglioloD. S., VilanovaM., CastelJ. R., GameroE., & ValdésE. (2021). Phenolic profile of grapevine cv. Tempranillo skins is affected by timing and severity of early defoliation. Spanish Journal of Agricultural Research, 19(3), e0905. https://doi.org/10.5424/sjar/2021193-17089
Section
Plant production (Field and horticultural crops)