Short communication: A potential role of tannins in the control of American foulbrood

  • Pablo Gimenez-Martinez Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM). CONICET-UNMdP. Centro de Asociación Simple CIC PBA, Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos aires
  • Matias Maggi Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM). CONICET-UNMdP. Centro de Asociación Simple CIC PBA, Mar del Plata
  • Sandra Fuselli Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM). CONICET-UNMdP. Centro de Asociación Simple CIC PBA, Mar del Plata Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos aires
Keywords: Paenibacillus larvae, antimicrobial activity, honeybee

Abstract

Aim of study: To evaluate the use of tannins extracts in the control of the American foulbrood pathology and to investigate if these extracts present levels of toxicity on Apis mellifera.

Area of study: Paenibacillus larvae strains C1 and C2 were from Balcarce, province of Buenos Aires, strain C6 from Rio Cuarto, in Cordoba province and strain C9 from Concordia in Entre Rios province. Bees larvae used for toxicological assays were collected in Santa Paula experimental apiary, Mar del Plata (belonging to the Centro de Investigación en Abejas Sociales (CIAS-IIPROSAM) from UNMdP.

Material and methods: The minimal inhibitory concentration (MIC) of five different tannin extracts were obtained by agar diffusion method on four P. larvae strains; using the MIC value, the toxicity test on A. mellifera larvae was performed afterwards.

Main results: The MIC value was in the range of 6.9 to 898.6 µg/mL. Three tannin extracts did not show toxicity against bee larvae; however, those that were fed with the latter showed a significant increase in weight.

Research highlights: Three tanninS extracts showed a good antimicrobial activity against P. larvae and they did not show toxicity against bee larvae.

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Published
2021-08-12
How to Cite
Gimenez-MartinezP., MaggiM., & FuselliS. (2021). Short communication: A potential role of tannins in the control of American foulbrood. Spanish Journal of Agricultural Research, 19(3), e05SC01. https://doi.org/10.5424/sjar/2021193-17628
Section
Animal health and welfare