Biotechnological approaches to develop nitrogen-fixing cereals: A review

  • Asma Boujenna Université Abdel Malek Essâadi, Faculté des Sciences, Dept. de Biologie, Av. Sebta, Tétouan 93002,
  • Luis F. Garcia del Moral University of Granada, Faculty of Sciences, Institute of Biotechnology, Dept. of Plant Physiology, Avda. Fuentenueva s/n, 18075 Granada
Keywords: nitrogenase, nif genes, O2 tolerance, plastids, mitochondria, plant growth-promoting rhizobacteria, biological nitrogen fixation

Abstract

Agricultural yields are often limited by nitrogen (N) availability, especially in countries of the developing world, whereas in industrialized nations the application of chemical N fertilizers has reached unsustainable levels that have resulted in severe environmental consequences. Finding alternatives to inorganic fertilizers is critical for sustainable and secure food production. Although gaseous nitrogen (N2) is abundant in the atmosphere, it cannot be assimilated by most living organisms. Only a selected group of microorganisms termed diazotrophs, have evolved the ability to reduce N2 to generate NH3 in a process known as biological nitrogen fixation (BNF) catalysed by nitrogenase, an oxygen-sensitive enzyme complex. This ability presents an opportunity to improve the nutrition of crop plants, through the introduction into cereal crops of either the N fixing bacteria or the nitrogenase enzyme responsible for N fixation. This review explores three potential approaches to obtain N-fixing cereals: (a) engineering the nitrogenase enzyme to function in plant cells; (b) engineering the legume symbiosis into cereals; and (c) engineering cereals with the capability to associate with N-fixing bacteria.

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Published
2021-10-27
How to Cite
BoujennaA., & Garcia del MoralL. F. (2021). Biotechnological approaches to develop nitrogen-fixing cereals: A review. Spanish Journal of Agricultural Research, 19(4), e08R01. https://doi.org/10.5424/sjar/2021194-18346
Section
Plant physiology