Use of CO2 balances to determine ventilation rates in a fattening rabbit house

  • F. Estelles Institute of Animal Science and Technology. Universitat Politècnica de València. Camino de Vera, 14. 46022 Valencia. Spain
  • N. Fernandez Institute of Animal Science and Technology. Universitat Politècnica de València. Camino de Vera, 14. 46022 Valencia. Spain
  • A. G. Torres Institute of Animal Science and Technology. Universitat Politècnica de València. Camino de Vera, 14. 46022 Valencia. Spain
  • S. Calvet Institute of Animal Science and Technology. Universitat Politècnica de València. Camino de Vera, 14. 46022 Valencia. Spain
Keywords: accuracy, animal welfare, climatization, emissions


Determining accurately the ventilation rates from rabbits houses, using non-expensive and non-invasive methods, is needed. The main aim of this work was to test the carbon dioxide balance as a method to determine the ventilation rate in fattening rabbit farms. In addition, the CO2 release rate from rabbit manure was measured, and the effect of CO2 concentration gradient between the inlet and outlet air of the building, on the method accuracy was characterized. To these aims, a fattening rabbit farm was evaluated during two periods and CO2 concentrations were simultaneously monitored in the inlet and outlet air by using a photoacoustic monitor. Ventilation rates were also directly determined by calibration of the exhaust fans and monitoring their operation times. CO2 emissions from manure were measured during two whole fattening periods, using a flux chamber and a photoacoustic monitor. The effect of CO2 concentrations gradient between the inlet and outlet of the farm on the accuracy of the balance was studied through statistical regressions. The CO2 emission from manure resulted in 13% of total CO2 emissions (considering both manure and animals). No statistically significant differences were found between measured and calculated ventilation rates. The effect of the CO2 gradient on the balance accuracy was statistically significant only in one of the trials. According to these results, the CO2 balance can be recommended for the determination of ventilation rates in fattening rabbit buildings.


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Ashrae, 2001. ASHRAE Fundamentals Handbook, printed edition. American Society of Heating, Refrigerating and Air-Conditioning Engineers Inc, Atlanta, GA, USA.

Blanes V., Pedersen S., 2005. Ventilation flow in pig houses measured and calculated by carbon dioxide, moisture and heat balance equations. Biosyst Eng 92(4), 483-493.

Calvet S., Cambra-López M., Blanes-Vidal V., Estellés F., Torres A.G., 2010. Ventilation rates in mechanically ventilated commercial poultry buildings in Southern Europe: measurement system development and uncertainty analysis. Biosyst Eng 106(4), 423-432.

Calvet S., Cambra-López M., Estellés F., Torres A.G., 2011. Characterisation of the indoor environment and gas emissions in rabbit farms. World Rabbit Sci 19(1), 49-61.

Chen Y., Barber E.M., Zhang Y., Besant R.W., Sokhansanj S., 1999. Methods to measure dust production and deposition rates in buildings. J Agr Eng Res 72(4), 329-340.

CIGR, 2002. Climatization of animal houses. Heat and moisture production at animal and house levels Pedersen S., Sälvik K., eds). Danish Inst of Agric Sci, Horsens, Denmark. pp. 1-46.

Estellés F., Calvet S., Blumetto O., Rodríguezlatorre A.R., Torres A.G., 2009. Technical Note: a flux chamber for measuring gas emissions from rabbits.World Rabbit Sci 17(3), 169-179.

Estellés F., Rodríguez-Latorre A.R., Calvet S., Villagrá A., Torres A.G., 2010. Daily carbon dioxide emission and activity of rabbits during the fattening period. Biosyst Eng 106(4), 338-343.

Hol J.M.G., Scheer A., Ogink N.W.M., 2004. Onderzoek naar de ammoniak- en geuremissie van stallen LX. Stal voor voedsters en vleeskonijnen. Report No. 219, Agrotechnology & Food Innovations BV Wageningen University, The Netherlands. 62 pp. [In Dutch].

Khalil M.H., Baselga M., 2002. Rabbit genetic resources in Mediterranean countries. CIHEAM Serie B:Études et recherches No. 38. Zaragoza, Spain.

Kiwull-Schöne H., Kalhoff H., Manz F., Diekmann L., Kiwull P., 2001. Minimal-invasive approach to study pulmonary, metabolic and renal responses to alimentary acid-base changes in conscious rabbits. Eur Jnutr 40(5), 255-259. PMid:11842951

Kiwull-Schöne H., Kalhoff H., Manz F., Kiwull P., 2005. Food mineral composition and acid-base balance in rabbits. Eur J Nutr 44(8), 499-508. PMid:15696401

Li H., Xin H., Liang Y., Gates R.S., Wheeler E.F.,Heber A.J., 2005. Comparison of direct vs. indirect ventilation rate determinations in layer barns using manure belts. T ASAE 48(1), 367-372.

Michl R., Hoy S., 1996. Results of continuous measuring of gases in rabbit keeping by using multigas-monitoring. Berliner und Munchener Tierarztliche Wochenschrift 109(9), 340-343. PMid:9054334

Ni J.Q., Heber A.J., 2008. Sampling and measurement of ammonia at animal facilities. Adv Agron 98, 201-69.

Pedersen S., Takai H., Johnsen J.O., Metz J.H.M., Koerkamp P.W.G.G., Uenk G.H., Phillips V.R., Holden M.R., Sneath R.W., Short J.L., White R.P., Hartung J., Seedorf J., Schroder M., Linkert K.H., Wathes C.M., 1998. A comparison of three balance methods for calculating ventilation rates in livestock buildings. J Agr Eng Res 70(1), 25-37.

Phillips V.R., Lee D.S., Scholtens R., Garland J.A., Sneath R.W., 2001. A review of methods for measuring emission rates of ammonia from livestock buildings and slurry on manure stores, Part 2: monitoring flux rates, concentrations and airflow rates. J Agr Eng Res 78(1), 1-14.

SAS, 2002. User's guide: statistics (Release 9.1.3). SAS®Institute Inc Cary, NC, USA.

Van Buggenhout S., Van Brecht A., Eren Özcan S., Vranken E., Van Malcot W., Berckmans D., 2009. Influence of sampling positions on accuracy of tracer gas measurements in ventilated spaces. Biosyst Eng 104(2), 216-223.

Van Ouwerkerk E.N.J., Pedersen S., 1994. Application of the carbon dioxide mass balance method to evaluate ventilation rates in livestock buildings. Proc XII World Congress on Agricultural Engineering. Milan, Italy, Aug 31-Sep 2. pp. 516-529.

Xin H., Li H., Burns R.T., Gates R.S., Overhults D.G., Earnest J.W., 2009. Use of CO2 concentration difference or CO2 balance to assess ventilation rate of broiler houses. T ASABE 52(4), 1353-1361.

How to Cite
EstellesF., FernandezN., TorresA. G., & CalvetS. (1). Use of CO2 balances to determine ventilation rates in a fattening rabbit house. Spanish Journal of Agricultural Research, 9(3), 713-720.
Agricultural engineering