Short communication. First report of Eggplant mottled dwarf virus in China rose in southern Spain

G. Parrella; A. De Stradis; B. Greco; F. Villanueva; I. M. Fortes; J. Navas-Castillo

doi:10.5424/sjar/2013111-3461

G. Parrella Istituto per la Protezione delle Piante del CNR, UOS di Portici, Via Università 133, 80055 Portici (NA)
A. De Stradis Istituto per la Virologia Vegetale del CNR, UOS di Bari, Via Amendola 165/a, 70126 Bari
B. Greco Istituto per la Protezione delle Piante del CNR, UOS di Portici, Via Università 133, 80055 Portici (NA)
F. Villanueva Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga
I. M. Fortes Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga
J. Navas-Castillo Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga

DOI: https://doi.org/10.5424/sjar/2013111-3461

Keywords: Hibiscus rosa-sinensis, EMDV, Nucleorhabdovirus, molecular probe, ELISA

Abstract

Eggplant mottled dwarf virus (EMDV, genus Nucleorhabdovirus, family Rhabdoviridae) is transmitted in nature by leafhoppers and its natural host range includes vegetable crops (eggplant, tomato, potato, pepper), ornamentals (pittosporum, honeysuckle, pelargonium) and wild plants (caper, Solanum nigrum). The prevalence of infections is generally very low. EMDV has been demonstrated to be the causal agent of a vein yellowing disease of China rose (Hibiscus rosa-sinensis) in southern Italy. In this work, four locations from Málaga and Granada provinces (southern Spain) were surveyed in 2011 to study the prevalence of EMDV infections in China rose by serological and molecular methods. Overall, EMDV was detected in 77.3% of the samples (33 out of 45 samples tested). Mechanical transmission tests and immunoelectron microscopy confirmed the presence of EMDV. The possible causes of such a high and unexpected prevalence are discussed. The use of molecular hibridization with an EMDV specific riboprobe is proposed for early screening of vegetative propagated China rose plants to avoid dissemination of infected material.

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Author Biography

G. Parrella, Istituto per la Protezione delle Piante del CNR, UOS di Portici, Via Università 133, 80055 Portici (NA)

Dipartimento Agroalimentare

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