Effect of different levels of raisin waste on performance, nutrients digestibility and protozoal population of Mehraban growing lambs

  • Vahid Saremi Dept. Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
  • Daryoush Alipour Dept. Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
  • Arash Azarfar Dept. of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad
  • Reza Sedighi Dept. Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
Keywords: rumen fermentation, ciliates, sheep, live weight gain, agro-industrial by-products


The aim of this study was to assess the effects of different inclusion levels of raisin waste (RW) in the diet on the animal performance and ruminal fermentation parameters of growing lambs. Four levels of RW inclusion (i.e., R0, R1, R2 and R3 for 0, 100, 200 and 300 g RW kg-1 dry matter of diet, respectively) were tested. The experimental diets were fed to 24 male lambs (six months old) and six animals were allocated to each treatment. In the first experiment, effects of different levels of RW on the animals’ performance, some rumen parameters and protozoa populations were studied. In the second experiment, the apparent total tract digestibility of diets and nitrogen balance were measured. The highest final body weights were observed for the R2 and R3 diets. The R3 diet had the lowest dry matter intake (1156 vs. 1303 g day-1 for R3 and R0, respectively) and feed conversion rate (6.4 vs. 8.7 for R3 and R0, respectively). Total number of protozoa increased with the addition of RW, but Epidinium spp. completely disappeared with the R3diet. Inclusion of RW at levels higher than 200 g RW kg-1 DM of diet significantly reduced crude protein (p=0.042) and neutral detergent fiber digestibility (p=0.049). Our findings showed that RW could be included in the diets of growing lambs up to 200 g kg-1 DM without compromising their production performance.


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Alipour D, Rouzbehan Y, 2007. Effects of ensiling grape pomace and addition of polyethyleneglycol on in vitro gas production and microbial biomass yield. Anim Feed Sci Technol 137: 138-149. http://dx.doi.org/10.1016/j.anifeedsci.2006.09.020

AOAC, 1995. Official methods of analysis, AOAC International, 2 vols. 16th edition. Arlington, VA, USA.

Bahrami Y, Foroozandeh AD, Zamani F, Modarresi M, Eghbal-Saeid S, Chekani-Azar S, 2010. Effect of diet with varying levels of dried grape pomace on dry matter digestibility and growth performance of male lambs. J Anim Plant Sci 6: 605-610.

Besharati M, Taghizadeh A, 2011. Effects of tannin binding agents (polyethylene glycol and polyvinylpyrrolidone) supplementation on in vitro gas production kinetics of grape yield byproducts. ISRN Vet Sci 1-8. http://dx.doi.org/10.5402/2011/780540

Broderick GA, Kang JH, 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 63: 64-75. http://dx.doi.org/10.3168/jds.S0022-0302(80)82888-8

Carulla JE, Kreuzer M, Machmller A, Hess HD, 2005. Supplementation of Acacia mearnsii tannins decreases methanogenesis and urinary nitrogen in forage-fed sheep. Aust J Agr Res 56: 961-970. http://dx.doi.org/10.1071/AR05022

Chiquette J, Cheng KJ, Rode LM, Milligan LP, 1989. Effect of tannin content in two isosynthetic strains of birdsfoot treefoil (Lotus corniculatus) on feed digestibility and rumen fluid composition in sheep. Can J Anim Sci 69: 1031-1039. http://dx.doi.org/10.4141/cjas89-117

Dabiri N, Thonney ML, 2004. Source and level of supplemental protein for growing lambs. J Anim Sci 82: 3237-3244.

Dawson LER, McCoy MA, Edgar HWJ, Carson AF, 2010. Effect of concentrate supplementation of condensed tannins (quebracho) in the concentrates on lamb performance and faecal egg and worm counts. Livest Sci 135: 205-214. http://dx.doi.org/10.1016/j.livsci.2010.07.006

Dehority BA, 1993.Laboratory manual for classification and morphology of rumen ciliate protozoa.CRC press, Boca Raton, FL, USA.

Dehority BA, 2003. Rumen microbiology. Nottingham Univ Press, Nottingham, UK.

France J, Dijkstra J, Dhanoa MS, Lopez S, Bannink A, 2000. Estimating the extent of degradation of ruminant feeds from a description of their gas production profile observed in vitro: derivation of models and other mathematical considerations. Br J Nutr 83: 143-150. http://dx.doi.org/10.1017/S0007114500000180

Hervas G, Perez V, Giraldez FJ, Mantecon AR, Almar MM, Frutos P, 2003. Intoxication of sheep with quebracho tannin extract. J Comp Pathol 129: 44-45. http://dx.doi.org/10.1016/S0021-9975(02)00168-8

Jones WT, Mangan LM, 1977.Complexes of the condensed tannins of sainfoin (Onobrychis viciifolia Scop.) with fraction 1 leaf protein and with submaxillary mucoprotein, and their reversal by polyethylene glycol and pH. J Sci Food Agr 28: 126-136. http://dx.doi.org/10.1002/jsfa.2740280204

Makkar HPS, 2003a. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effect of feeding tannin-rich feeds. Small Rumin Res 49: 241-256. http://dx.doi.org/10.1016/S0921-4488(03)00142-1

Makkar HPS, 2003b. Quantification of tannins in tree and shrub foliage: a laboratory manual. Joint FAO/IAEA, Division of Nuclear Techniques in Food and Agriculture. Kluwer Acad Publ, Dordrecht, The Netherlands.

Makkar HPS, Becker K, Younan M, 1997. Nutritional implications of bound proanthocyanidins. Proc XVIII Int Grassland Cong, June 8-12, Winnipeg, Canada.

Markham R, 1942. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem J 36: 790-790.

Mass RA, Lardy GP, Grant RJ, Klopfenstein TJ, 1999. In situ neutral detergent insoluble nitrogen as a method for measuring forage protein degradability. J Anim Sci 77: 1565-1571.

McNabb WC, Waghorn GC, Peters JS, Barry TN, 1996. The effect of condensed tannins in Lotus pedunculatus upon the solubilization and degradation of ribulose-1,5-bisphosphate carboxylase protein in the rumen and on sites of digestion. Br J Nutr 76: 535-549. http://dx.doi.org/10.1079/BJN19960061

Menke KH, Steingass H, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Develop 28: 7-55.

Michalowski T, Rybicka K, Wereszka K, Kasperowicz A, 2001. Ability of the rumen ciliate Epidinium ecaudatum to digest and use crystalline cellulose and xylan for in vitro growth. Acta Protozoologica 40: 203-210.

Min BR, Barry TN, Attwood GT, McNabb WC, 2003. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Anim Feed Sci Technol 106: 3-19. http://dx.doi.org/10.1016/S0377-8401(03)00041-5

Miron J, Ben-Ghedalia D, Morrison M, 2001. Adhesion mechanisms of rumen cellulolytic bacteria. J Diary Sci. 84: 1294-1309. http://dx.doi.org/10.3168/jds.S0022-0302(01)70159-2

NRC, 1985. Nutrient requirements of sheep, 6th ed. Nat Acad Press, Washington DC, USA.

Patra AK, Saxena J, 2009. Dietary phytochemicals as rumen modifiers: a review of the effects on microbial populations. Antonie van Leeuwenhoek 96: 363-375. http://dx.doi.org/10.1007/s10482-009-9364-1

Priolo A, Waghorn GC, Lanza M, Biondi L, Pennisi P, 2000. Polyethylene glycol as a means for reducing the impact of condensed tannins in carob pulp: effects on lamb growth, performance and meat quality. J Anim Sci 78: 810-816.

Oldham JD, 1984. Protein-energy interrelationships in dairy cows. J Dairy Sci 67: 1090-1114. http://dx.doi.org/10.3168/jds.S0022-0302(84)81410-1

Reed JD, 1995. Nutritional toxicology of tannins and related polyphenols in forage legumes. J Anim Sci 73: 1516-1528.

Roffler RE, Wray JE, Satter LD, 1986. Production responses in early lactation to additions of soybean meal to diets containing predominantly corn silage. J Dairy Sci 69: 1055-1062. http://dx.doi.org/10.3168/jds.S0022-0302(86)80501-X

Śliwinśki BJ, Kreuzer M, Sutter F, Machmüller A, Wettstein HR, 2004. Performance, body nitrogen conversion and nitrogen emission from manure of dairy cows fed diets supplemented with different plant extracts. J Anim Feed Sci 13: 75-94.

Takenaka A, Tajima K, Mitsumori M, Kajikawa H, 2004.Fiber digestion by rumen ciliate protozoa. Microbes Environ 19: 203-210. http://dx.doi.org/10.1264/jsme2.19.203

Van Soest P, 1994. Nutritional ecology of the ruminant. Cornell Univ Press, Ithaca, NY, USA.

Van Soest PJ, Robertson J, Lewis BA, 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 74: 3583-3597. http://dx.doi.org/10.3168/jds.S0022-0302(91)78551-2

Waghorn GC, Jones WT, Shelton ID, McNabb WC, 1990. Condensed tannins and the nutritive value of herbage. Proc NZ Grass Assoc 51: 171-176.

Wang Y, Waghorn GC, McNabb WC, Barry TN, Hedley M, Shelton I, 1996. Effect of condensed tannins in Lotus corniculatus upon the digestion of methionine and cystine in the small intestine of sheep. J Agr Sci 127: 413-421. http://dx.doi.org/10.1017/S0021859600078576

How to Cite
SaremiV., AlipourD., AzarfarA., & SedighiR. (2014). Effect of different levels of raisin waste on performance, nutrients digestibility and protozoal population of Mehraban growing lambs. Spanish Journal of Agricultural Research, 12(1), 159-166. https://doi.org/10.5424/sjar/2014121-4613
Animal production