Dose-dependent effect of a single GnRHa injection on the spawning of meagre (Argyrosomus regius) broodstock reared in captivity

  • Hipolito Fernandez-Palacios Universidad de Las Palmas de Gran Canaria (ULPGC). Grupo de Investigación en Acuicultura (GIA). Muelle de Taliarte S/N, 35214 Telde (Las Palmas)
  • Dominique Schuchardt Universidad de Las Palmas de Gran Canaria (ULPGC). Grupo de Investigación en Acuicultura (GIA). Muelle de Taliarte S/N, 35214 Telde (Las Palmas)
  • Javier Roo Universidad de Las Palmas de Gran Canaria (ULPGC). Grupo de Investigación en Acuicultura (GIA). Muelle de Taliarte S/N, 35214 Telde (Las Palmas)
  • Marisol Izquierdo Universidad de Las Palmas de Gran Canaria (ULPGC). Grupo de Investigación en Acuicultura (GIA). Muelle de Taliarte S/N, 35214 Telde (Las Palmas)
  • Carmen Hernandez-Cruz Universidad de Las Palmas de Gran Canaria (ULPGC). Grupo de Investigación en Acuicultura (GIA). Muelle de Taliarte S/N, 35214 Telde (Las Palmas)
  • Neil Duncan IRTA, Sant Carles de la Rápita, Ctra. Poble Nou km 5.5, 43540 Sant Carles de la Rapita (Tarragona)
Keywords: fish reproduction, hormonal induction, egg quality


The present study aimed to determine the spawning efficacy, egg quality and quantity of captive breed meagre induced with a single gonadotrophin‐releasing hormone agonist (GnRHa) injection of 0, 1, 5, 10, 15, 20, 25, 30, 40 or 50 µg kg-1 to determine a recommended optimum dose to induce spawning. The doses 10, 15 and 20 μg kg-1 gave eggs with the highest quality (measured as: percentage of viability, floating, fertilisation and hatch) and quantity (measured as: total number of eggs, number of viable eggs, number of floating eggs, number of hatched larvae and number of larvae that reabsorbed the yolk sac). All egg quantity parameters were described by Gaussian regression analysis with R2=0.89 or R2=0.88. The Gaussian regression analysis identified that the optimal dose used was 15 μg kg-1. The regression analysis highlighted that this comprehensive study examined doses that ranged from low doses insufficient to stimulate a high spawning response (significantly lower egg quantities, p<0.05) compared to 15 μg kg-1 through to high doses that stimulated the spawning of significantly lower egg quantities and eggs with significantly lower quality (egg viability). In addition, the latency period (time from hormone application to spawning) decreased with increasing doses to give a regression (R2=0.93) which suggests that higher doses accelerated oocyte development that in turn reduced egg quality and quantity. The identification of an optimal dose for the spawning of meagre, which has high aquaculture potential, represents an important advance for the Mediterranean aquaculture industry.


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Abou Shabana NM, Abd El Rahma SH, Al Absawy MA, Assem SS, 2012. Reproductive biology of Argyrosomus regius (Asso, 1801) inhabiting the south eastern Mediterranean Sea, Egypt. Egypt J Aquac Res 38: 147-156.

Barbaro A, Francescon A, Bozzato G, Merlin A, Belvedere P, Colombo L, 1997. Induction of spawning in gilthead seabream, Sparus aurata L., by a long-acting GnRH agonist and its effects on egg quality and daily timing of spawning. Aquaculture 197: 349-359.

Billard R, 1986. Spermatogenesis and spermatology of some teleost fish. Reprod Nutr Dev 26: 877-920.

Billard R, 1989. Endocrinology and fish culture. Fish Physiol Biochem 7: 49-58.

Clearwater SJ, Pankhurst NW, 1997. The response to capture and confinement stress of plasma cortisol, plasma sex steroids and vitellogenic oocytes in the marine teleost, red gurnard. J Fish Biol 50: 429-441.

Cleary JJ, Pankhurst NW, Battaglene SC, 2000. The effect of capture and handling stress on plasma steroid levels and gonadal condition in wild and farmed snapper Pagrus auratus (Sparidae). J World Aquacult Soc 31: 558-569.

Duncan NJ, Rodriguez M, Alok D, Zohar Y, 2003. Effects of controlled delivery and acute injections of LHRHa on bullseye puffer fish (Sphoeroides annulatus) spawning. Aquaculture 218: 626-635.

Duncan N, Estevez A, Padros F, Aguilera C, Montero FE, Norambuena F, Carazo I, Carbo R, Mylonas CC, 2008. Acclimation to captivity and GnRHa-induced spawning of meagre (Argyrosomus regius). Cybium 32: 332-333.

Duncan N, Estevez A, Porta J, Carazo I, Norambuena F, Aguilera C, Gairin I, Bucci F, Valles R, Mylonas CC, 2012. Reproductive development, GnRHa-induced spawning and egg quality of wild meagre (Argyrosomus regius) acclimatised to captivity. Fish Physiol Biochem 38: 1273-1286.

Duncan NJ, Estevez A, Fernández-Palacios H, Gairin I, Hernández-Cruz CM, Roo J, Schuchardt D, Vallés R, 2013. Aquaculture production of meagre (Argyrosomus regius): hatchery techniques, ongrowing and market. In: Advances in aquaculture hatchery technology, vol 242 (Allan G & Burnell G, eds). Woodhead Publishing Limited, Cambridge UK, pp: 519-541.

EATIP, 2012. The future of European Aquaculture: a strategic agenda for research and innovation. Available in [18 June 2013].

Fernández-Palacios H, Izquierdo MS, Robaina L, Valencia A. Salhi M, Vergara JM, 1995. Effect of n-3 HUFA level in broodstock diets on egg quality of gilthead sea bream (Sparus aurata L.). Aquaculture 132: 325-337.

Fornies MA, Mañanos E, Carrillo M, Rocha A, Laureau S, Mylonas CC, Zohar Y, Zanuy S, 2001. Spawning induction of individualized European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems. Aquaculture 202: 221-234.

García LMB, 1989. Dose-dependent spawning response of mature female sea bass, Lates calcarifer (Bloch), to pelleted luteinizing hormone-releasing hormone analogue (LHRHa). Aquaculture 77: 85-96.

Gil MM, Grau A, Basilone G, Ferreri R, Palmer M, 2013. Reproductive strategy and fecundity of meagre Argyrosomus regius Asso, 1801 (Pisces: Sciaenidae): implications for restocking programs. Sci Mar 77: 105-118.

Ibarra-Castro L, Duncan NJ, 2007. GnRHa-induced spawning of wild-caught spotted rose snapper Lutjanus guttatus. Aquaculture 272: 737-746.

Mañanos E, Carrillo M, Sorber LA, Mylonas CC, Asturiano JF, Bayarri MJ, Zohar Y, Zanuy S, 2002. Luteinizing hormone and sexual steroid plasma levels after treatment of European sea bass with sustained-release delivery systems for gonadotropin-releasing hormone analogue. J Fish Biol 60: 328-339.

Mañanos E, Duncan NJ, Mylonas CC, 2008. Reproduction and control of ovulation, spermiation and spawning in cultured fish. In: Methods in reproductive aquaculture (Cabrita E, Robles V, Herraez P, eds). CRC Press, Boca Raton, FL, USA, pp: 1-83.

Monfort MC, 2010. Present market situation and prospects of meagre (Argyrosomus regius), as an emerging species in Mediterranean aquaculture, studies and reviews. General Fisheries Commission for the Mediterranean No. 89, FAO, Roma, pp: 1-28.

Morehead DT, 1998. Effect of capture, confinement and repeated sampling on plasma steroid concentrations and oocyte size in female striped trumpeter Latris lineata (Latrididae). Mar Freshwater Res 49: 373-377.

Mugnier C, Guennoc M, Lebegue E, Fostier A, Breton B, 2000. Induction and synchronisation of spawning in cultivated turbot (Scophthalmus maximus L.) broodstock by implantation of a sustained-release GnRH-a pellet. Aquaculture 18: 241-255.

Mylonas CC, Zohar Y, 2001. Use of GnRHa-delivery systems for the control of reproduction in fish. Rev Fish Biol Fisher 10: 463–491.

Mylonas CC, Hinshaw JM, Sullivan CV, 1992. GnRHa-induced ovulation of brown trout (Salmo salar) and its effects on egg quality. Aquaculture 106: 379–392.

Mylonas CC, Sigelaki I, Divanach P, Mañanos E, Zanuy S, Afonso-Polyviou A, 2003. Multiple spawning of individual European sea bass (Dicentrarchus labrax) after repeated injections of GnRHa, and evaluation of egg quality. Aquaculture 221: 605-620.

Mylonas CC, Kyriakou Y, Sigelaki I, Georgiou G, Stephanou D, Divanach P, 2004. Reproductive biology of the shi drum (Umbrina cirrosa) in captivity and induction of spawning using GnRHa. Isr J Aquacult-Bamid 56: 75-92.

Mylonas CC, Fostier A, Zanuy S, 2010. Broodstock management and hormonal manipulations of fish reproduction. Gen Comp Endocr 165: 516–534.

Mylonas CC, Mitrizakis N, Sigelaki I, Papadaki M, 2011. Spawning kinetics of individual female meagre (Argyrosomus regius) after treatment with GnRHa implants. Proc 9th Int Symp on Reproductive Physiology of Fish, Cochin, India. 9–14 August 2011.

Mylonas CC, Mitrizakis N, Castaldo CA, Cerviño CP, Papadaki M, Sigelaki I, 2013a. Reproduction of hatchery-produced meagre Argyrosomus regius in captivity II. Hormonal induction of spawning and monitoring of spawning kinetics, egg production and egg quality. Aquaculture 414-415: 318-327.

Mylonas CC, Mitrizakis N, Papadaki M, Sigelaki I, 2013b. Reproduction of hatchery-produced meagre Argyrosomus regius in captivity I. Description of the annual reproductive cycle. Aquaculture 414-415: 309-317.

Peter RE, Lin HR, Van Der Kraak G, Little M, 1993. Releasing hormones, dopamine antagonists and induced spawning. Recent Adv Aquacult 4: 25-30.

Podhorec P, Socha M, Sokolowska-Mikolajczyk ME, Drozd B, Policar T, Stejskal V, Kouril J, 2011. Effective dose of mGnRHa for induction of ovulation in tench (Tinca tinca L.). Aquaculture 319: 184–187.

Podhorec P, Socha M, Sokolowska-Mikolajczyk ME, Policar T, Svinger VW, Drozd B, Kouril J, 2012. Dopamine control of LH release in the tench (Tinca tinca). Gen Comp Endocrinol 175: 34-38.

Schiavone R, Zilli L, Storelli C, Vilella S, 2012. Changes in hormonal profile, gonads and sperm quality of Argyrosomus regius (Pisces, Scianidae) during the first sexual differentiation and maturation. Theriogenology 77: 888–898.

Shearer KD, 2000. Experimental design, statistical analysis and modelling of dietary nutrient requirement studies for fish: a critical review. Aquacult Nutr 6: 91–102.

Sokal RR, Rolf J, 1996. Biometría: Principios y métodos estadísticos en la investigación biológica. Editorial Blume, Madrid, 832 pp.

Taranger GL, Stefansson SO, Hansen T, 1992. Advancement and synchronization of ovulation in atlantic salmon (Salmo salar L) following injections of LHRH analog. Aquaculture 102: 169-175.

Wang Y, Hu M, Wang W, Li X, Cheung SG, Shin PKS, Song L, 2009. Effects of GnRHa (D-Ala6, Pro9-NEt) combined with domperidone on ovulation induction in wild loach Misgurnus anguillicaudatus. Aquaculture 291: 136-139.

Yang Z, Chen Y, 2004. Induced ovulation in obscure puffer Takifugu obscurus by injections of LHRHa. Aquac Int 12: 215–223.

Zohar Y, 1988. Gonadotropin releasing hormone in spawning induction in teleosts: basic and applied considerations. In: Reproduction in fish: basic and applied aspects in endocrinology and genetics (Zohar Y & Breton B, eds). INRA Press, París, pp: 47-62.

Zohar Y, 1989a. Fish reproduction: its physiology and artificial manipulation. In: Fish culture in warm water systems: problems and trends (Shilo M & Saríg S, eds). CRC Press, Boca Raton, pp: 65-119.

Zohar Y, 1989b. Endocrinology and fish farming: aspects in reproduction growth, and smoltification. Fish Physiol Biochem 7: 395-405.

Zohar Y, Mylonas CC, 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197: 99-136.

Zohar Y, Harel M, Hassin S, Tandler A, 1995. Gilt-head sea bream (Sparus aurata). In: Broodstock management and egg and larval quality (Bromage NR & Roberts RJ, eds). Blackwell, Oxford, UK, pp: 94–117.

How to Cite
Fernandez-PalaciosH., SchuchardtD., RooJ., IzquierdoM., Hernandez-CruzC., & DuncanN. (2014). Dose-dependent effect of a single GnRHa injection on the spawning of meagre (Argyrosomus regius) broodstock reared in captivity. Spanish Journal of Agricultural Research, 12(4), 1038-1048.
Animal breeding, genetics and reproduction