Nitric oxide and nitrous oxide emissions from cattle-slurry and mineral fertiliser treated with nitrification inhibitor to an agricultural soil: A laboratory approach

  • José Pereira (1) CI&DETS, Agrarian School of Viseu, Polytechnic Institute of Viseu, Quinta da Alagoa, 3500-606 Viseu (2) Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB, University of Trás-os-Montes and Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real
  • João Coutinho Chemistry Centre, University of Trás-os-Montes and Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real
  • David Fangueiro LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa
  • Henrique Trindade Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB, University of Trás-os-Montes and Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real
Keywords: carbon dioxide, DMPP, N emissions, N mineralisation, solid-liquid separation


The application of organic and mineral fertilisers to soil can result in increased gaseous emissions to the atmosphere such as nitric oxide (NO) and nitrous oxide (N2O) gases. The aim of this study was to evaluate under laboratory conditions the effects on mineral N dynamics and NO and N2O emissions of application to soil of cattle slurry derived liquid fraction (LF) obtained by screw press and mineral fertiliser (MF), both treated with or without the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP). An aerobic laboratory incubation was performed over 93 days with a Dystric Cambisol amended with mechanically separated LF or mineral fertiliser ammonium sulphate only or combined with DMPP. Two additional treatments were included: soil only and soil amended with DMPP. Nitrogen immobilisation was the dominant process with MF amendment, whereas N mineralisation has been observed with LF. The application of LF reduced significantly NO emissions by 80% relative to mineral but no differences were observed with N2O emissions. The addition of DMPP to MF induced a decrease of 18 and 29% in NO and N2O emissions whereas DMPP combined with LF reduced (numerically but not statistically) these emissions in 20 and 10%, respectively. Results obtained in our study suggest that N (NO + N2O) losses can be mitigated by adding DMPP to mineral fertilisers or replacing mineral fertiliser by LF.


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How to Cite
PereiraJ., CoutinhoJ., FangueiroD., & TrindadeH. (2015). Nitric oxide and nitrous oxide emissions from cattle-slurry and mineral fertiliser treated with nitrification inhibitor to an agricultural soil: A laboratory approach. Spanish Journal of Agricultural Research, 13(4), e0305.
Agricultural environment and ecology